Article
Biochemistry & Molecular Biology
Anton Golovnin, Larisa Melnikova, Valentin Babosha, Galina V. Pokholkova, Ivan Slovohotov, Anastasia Umnova, Oksana Maksimenko, Igor F. Zhimulev, Pavel Georgiev
Summary: The N-terminal region of CP190 plays an essential role in recruiting various DNA-binding architectural proteins and transcription complexes, and is crucial for the recruitment to regulatory regions associated with architectural proteins and achieving chromatin opening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chikara Takeuchi, Moe Yokoshi, Shu Kondo, Aoi Shibuya, Kuniaki Saito, Takashi Fukaya, Haruhiko Siomi, Yuka W. Iwasaki
Summary: In Drosophila, specific splice variants of the insulator protein Mod(mdg4)-N repress HeT-A expression by blocking subtelomeric enhancers and associating with the subtelomeric sequences. Furthermore, Mod(mdg4)-N recruits RNA polymerase II to the subtelomeres, reinforcing its enhancer-blocking function.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Anjali Kaushal, Julien Dorier, Bihan Wang, Giriram Mohana, Michael Taschner, Pascal Cousin, Patrice Waridel, Christian Iseli, Anastasiia Semenova, Simon Restrepo, Nicolas Guex, Erez Lieberman Aiden, Maria Cristina Gambetta
Summary: Boundaries in animal genomes play a crucial role in limiting regulatory cross-talk and guiding enhancers to target promoters. This study reveals that the formation and function of boundaries in flies are different from mammals. Unlike mammalian boundaries, most Drosophila boundaries form independently of CTCF and are recruited by Cp190. These boundaries are essential for early development and prevent regulatory cross-talk between specific gene loci, but are dispensable for long-range enhancer-promoter communication.
Article
Genetics & Heredity
Indira Bag, Yang Chen, Karole D'Orazio, Prisma Lopez, Sabine Wenzel, Yuichiro Takagi, Elissa P. Lei
Summary: Chromatin insulators are DNA-protein complexes that establish independent transcriptional domains in the genome. This study reveals a novel RNA-binding protein, Isha, that interacts with the su(Hw) mRNA and associates with the gypsy chromatin insulator complex. Isha interacts with RNA Polymerase II and is involved in the RNA-dependent chromatin association at the 5' end of genes. Depletion of Isha disrupts the enhancer-blocking and barrier activities of the gypsy insulator, as well as the nuclear localization of insulator bodies.
G3-GENES GENOMES GENETICS
(2022)
Article
Cell Biology
Marta Marzullo, Giulia Romano, Claudia Pellacani, Federico Riccardi, Laura Ciapponi, Fabian Feiguin
Summary: Aging modifies the physiological balance of the organism, increasing susceptibility to neurodegenerative diseases. This is related to epigenetic chromatin remodeling events that affect the expression levels of disease-causing genes and neuronal survival.
CELL DEATH DISCOVERY
(2023)
Article
Genetics & Heredity
M. M. Erokhin, F. V. Gorbenko, D. V. Lomaev, D. A. Chetverina
Summary: Polycomb and Trithorax group proteins are responsible for transcriptional repression and activation in fruit flies. They are recruited to DNA elements called Polycomb response elements. The binding sites of architectural proteins can induce the repressive activity of specific DNA elements and recruit Polycomb/Trithorax group proteins, regardless of the presence of other DNA sequences.
RUSSIAN JOURNAL OF GENETICS
(2023)
Article
Biochemistry & Molecular Biology
D. A. Chetverina, F. Gorbenko, D. Lomaev, P. G. Georgiev, M. M. Erokhin
Summary: Polycomb group (PcG) and Trithorax group (TrxG) proteins interact with Polycomb response elements (PRE) to regulate gene expression in Drosophila. The presence of architectural protein-binding sites induces the repressive activity of inactive PRE. This study shows that the recruitment of GAF and Psq to bxdPRE is dependent on the presence of adjacent binding sites for architectural proteins.
DOKLADY BIOCHEMISTRY AND BIOPHYSICS
(2022)
