Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability

(2016) Hana Antonicka et al.   EMBO REPORTS

Tobacco Etch Virus protease: A shortcut across biotechnologies

(2016) Francesca Cesaratto et al.   JOURNAL OF BIOTECHNOLOGY

A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

(2016) Jun Chu et al.   NATURE BIOTECHNOLOGY

Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown

(2016) Mihaela Pertea et al.   Nature Protocols

A scalable strategy for high-throughput GFP tagging of endogenous human proteins

(2016) Manuel D. Leonetti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

(2016) Bryce T. Bajar et al.   Scientific Reports

HEK293 in cell biology and cancer research: phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolution

(2015) A.A. Stepanenko et al.   GENE

DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts

(2015) Teresa Szczepińska et al.   GENOME RESEARCH

Effects of Transcription Elongation Rate and Xrn2 Exonuclease Activity on RNA Polymerase II Termination Suggest Widespread Kinetic Competition

(2015) Nova Fong et al.   MOLECULAR CELL

Rapid, optimized interactomic screening

(2015) Zhanna Hakhverdyan et al.   NATURE METHODS

hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0

(2015) Rafal Tomecki et al.   RNA Biology

The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs

(2015) Benedikt M. Beckmann et al.   Nature Communications

High variability of genomic instability and gene expression profiling in different HeLa clones

(2015) Annalisa Frattini et al.   Scientific Reports

Label-free quantitative proteomic analysis of the YAP/TAZ interactome

(2014) Priyanka Kohli et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

THOC5, a member of the mRNA export complex: a novel link between mRNA export machinery and signal transduction pathways in cell proliferation and differentiation

(2014) Doan D H Tran et al.   Cell Communication and Signaling

Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein

(2014) Jun Chu et al.   NATURE METHODS

Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate

(2014) Nela Durisic et al.   NATURE METHODS

Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling

(2014) C. C. Williams et al.   SCIENCE

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

(2014) Yao-Cheng Lin et al.   Nature Communications

Challenges and opportunities in the purification of recombinant tagged proteins

(2013) Ana Sofia Pina et al.   BIOTECHNOLOGY ADVANCES

Mapping the Human miRNA Interactome by CLASH Reveals Frequent Noncanonical Binding

(2013) Aleksandra Helwak et al.   CELL

Single cell heterogeneity

(2013) Batoul Y Abdallah et al.   CELL CYCLE

Interaction of PACAP with Sonic hedgehog reveals complex regulation of the Hedgehog pathway by PKA

(2013) Pawel Niewiadomski et al.   CELLULAR SIGNALLING

The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line

(2013) Andrew Adey et al.   NATURE

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target

(2013) R. Tomecki et al.   NUCLEIC ACIDS RESEARCH

A Practical Comparison of Ligation-Independent Cloning Techniques

(2013) Julian Stevenson et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Live-Cell Visualization of Pre-mRNA Splicing with Single-Molecule Sensitivity

(2013) Robert M. Martin et al.   Cell Reports

The Genomic and Transcriptomic Landscape of a HeLa Cell Line

(2013) Jonathan J. M. Landry et al.   G3-Genes Genomes Genetics

Structure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206K

(2012) David von Stetten et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

(2012) Jae-Yeon Jeong et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A simple strand-specific RNA-Seq library preparation protocol combining the Illumina TruSeq RNA and the dUTP methods

(2012) Marc Sultan et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

STAR: ultrafast universal RNA-seq aligner

(2012) Alexander Dobin et al.   BIOINFORMATICS

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications

(2012) Carissa L. Young et al.   Biotechnology Journal

Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

(2012) Alfredo Castello et al.   CELL

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

(2012) Kyle J. Roux et al.   JOURNAL OF CELL BIOLOGY

Alternative translation initiation augments the human mitochondrial proteome

(2012) Lawrence Kazak et al.   NUCLEIC ACIDS RESEARCH

Counting single photoactivatable fluorescent molecules by photoactivated localization microscopy (PALM)

(2012) S.-H. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Using Cell-to-Cell Variability--A New Era in Molecular Biology

(2012) L. Pelkmans   SCIENCE

Quantitative Proteomics Reveals the Basis for the Biochemical Specificity of the Cell-Cycle Machinery

(2011) Felicia Walton Pagliuca et al.   MOLECULAR CELL

Deep proteome and transcriptome mapping of a human cancer cell line

(2011) N. Nagaraj et al.   Molecular Systems Biology

PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria

(2011) Joanna Rorbach et al.   NUCLEIC ACIDS RESEARCH

A Rapid Flp-In System for Expression of Secreted H5N1 Influenza Hemagglutinin Vaccine Immunogen in Mammalian Cells

(2011) Hanxin Lu et al.   PLoS One

Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40

(2011) Anton Kuzmenko et al.   Scientific Reports

An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio

(2010) Yutaka Kodama* et al.   BIOTECHNIQUES

Crosstalk between mRNA 3′ End Processing and Transcription Initiation

(2010) Christophe K. Mapendano et al.   MOLECULAR CELL

Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition

(2010) Mateusz Wydro et al.   NUCLEIC ACIDS RESEARCH

Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter

(2010) Jane Yuxia Qin et al.   PLoS One

The nucleolus: structure/function relationship in RNA metabolism

(2010) Danièle Hernandez-Verdun et al.   Wiley Interdisciplinary Reviews-RNA

In vivo gene regulation using tetracycline-regulatable systems

(2009) Knut Stieger et al.   ADVANCED DRUG DELIVERY REVIEWS

The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine

(2009) Ashley J. Pratt et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

APE1/Ref-1 Interacts with NPM1 within Nucleoli and Plays a Role in the rRNA Quality Control Process

(2009) C. Vascotto et al.   MOLECULAR AND CELLULAR BIOLOGY

The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome

(2009) Patrizia Fabrizio et al.   MOLECULAR CELL

Structure and function of the 5′→3′ exoribonuclease Rat1 and its activating partner Rai1

(2009) Song Xiang et al.   NATURE

Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components

(2009) Zbigniew Warkocki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Overlapping promoter targeting by Elk-1 and other divergent ETS-domain transcription factor family members

(2009) Joanna Boros et al.   NUCLEIC ACIDS RESEARCH

Inducible expression of coding and inhibitory RNAs from retargetable genomic loci

(2009) Ina Weidenfeld et al.   NUCLEIC ACIDS RESEARCH

The Cajal body

(2008) Glenn E. Morris   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Conversion of Red Fluorescent Protein into a Bright Blue Probe

(2008) Oksana M. Subach et al.   CHEMISTRY & BIOLOGY

Visualization of ternary complexes in living cells by using a BiFC-based FRET assay

(2008) Y John Shyu et al.   Nature Protocols

Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus

(2008) Edward Emmott et al.   VIROLOGY

Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts

(2007) Heath E. Klock et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS