Review
Biochemistry & Molecular Biology
Hongyong Shi, Xiaopeng Li, Minghui Lv, Jia Li
Summary: The BES1/BZR1 family is a plant-specific group of transcription factors that regulate the expression of brassinosteroid response genes. Recent studies have shown that they also play a role in plant development through pathways independent of brassinosteroids. This review summarizes our current understanding of their mechanisms, DNA binding motifs, and target genes, as well as their biological functions via both brassinosteroid-dependent and independent pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jing Cao, Yuxia Liang, Tingting Yan, Xuncheng Wang, Hua Zhou, Chen Chen, Yingli Zhang, Beihong Zhang, Shuhao Zhang, Juncheng Liao, Shujing Cheng, Jinfang Chu, Xi Huang, Dongqing Xu, Jia Li, Xing Wang Deng, Fang Lin
Summary: The photomorphogenic repressors BBX28 and BBX29 integrate light and brassinosteroid signaling by promoting BR-ENHANCED EXPRESSION transcriptional activity to precisely regulate seedling development. BBX28 and BBX29 physically interact with BEE1, BEE2, and BEE3 to enhance their binding to and activation of target genes, thus participating in the coordination of light and BR signaling.
Article
Biochemistry & Molecular Biology
Yui Otani, Mika Kawanishi, Miyu Kamimura, Azusa Sasaki, Yasushi Nakamura, Takako Nakamura, Shigehisa Okamoto
Summary: The study found that BEH2 was highly expressed in the roots and leaf margins, which was significantly different from another member BEH4. BRs downregulated the expression of BEH2 through a module containing GSK3-like kinases and BES1 transcription factors, with BES1 playing a more important role in this process than BZR1. BEH2 consistently existed in the nucleus and its subcellular localization was not controlled by BR-dependent nuclear-cytoplasmic shuttling. Furthermore, gene ontology analysis suggested that BEH2 may be involved in stress response and photosynthesis.
PLANT SIGNALING & BEHAVIOR
(2022)
Article
Cell Biology
Dawei Zhang, Wenrong Tan, Feng Yang, Qing Han, Xingguang Deng, Hongqing Guo, Baohui Liu, Yanhai Yin, Honghui Lin
Summary: GSK3-like kinases in Arabidopsis play roles in plant development, including chloroplast development, and are regulated by BIN2 through phosphorylation. The interaction between BIN2 and GLK1/2 affects chloroplast development and responses to BRs/darkness, modulating photomorphogenesis.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Wenbin Zhang, Yang Tang, Yilong Hu, Yuhua Yang, Jiajia Cai, Hailun Liu, Chunyu Zhang, Xu Liu, Xingliang Hou
Summary: NF-YC proteins act as essential repressors of the BR pathway during light-controlled hypocotyl growth in Arabidopsis, inhibiting BR biosynthesis and signaling to ensure photomorphogenic growth.
Article
Plant Sciences
Nevedha Ravindran, Harshil Ramachandran, Nikhil Job, Arpita Yadav, K. P. Vaishak, Sourav Datta
Summary: BBX32 negatively regulates light signaling and promotes BR signaling to inhibit cotyledon opening in Arabidopsis. It integrates light and BR signals to regulate cotyledon opening during de-etiolation, interacting with both BZR1 and PIF3 to modulate the expression of common target genes.
Article
Plant Sciences
Guohua Chai, Guang Qi, Dian Wang, Yamei Zhuang, Hua Xu, Zetao Bai, Ming-Yi Bai, Ruibo Hu, Zeng-Yu Wang, Gongke Zhou, Yingzhen Kong
Summary: This study reveals the negative regulatory mechanism of the Arabidopsis CCCH protein C3H15 in cell elongation through inhibiting brassinosteroid signaling. C3H15 functions downstream of the BRI1 receptor as a negative regulator in the BR pathway. It is phosphorylated by the kinase BIN2 and its dephosphorylation leads to its accumulation in the nucleus, where it regulates transcription of G-rich elements. C3H15 and the transcriptional regulators BZR1 and BES1 antagonize each other and share common BR-responsive target genes.
Article
Plant Sciences
Su-Kui Jin, Ming-Qiu Zhang, Yu-Jia Leng, Li-Na Xu, Shu-Wen Jia, Shui-Lian Wang, Tao Song, Ruo-An Wang, Qing-Qing Yang, Tao Tao, Xiu-Ling Cai, Ji-Ping Gao
Summary: OsNAC129 is a gene associated with seed development and plant growth. Loss-of-function of OsNAC129 increases grain length, grain weight, and apparent amylose content, while overexpression of OsNAC129 has the opposite effect. This gene negatively regulates grain size and starch biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jian-Ping An, Zhi-Ying Liu, Xiao-Wei Zhang, Da-Ru Wang, Fanchang Zeng, Chun-Xiang You, Yuepeng Han
Summary: In apple, a key component of BR signaling, MdBIM1, increases cold tolerance by activating the expression of CBF genes and forming complexes with MdCBF2. The repressors of JA signaling, MdJAZ1 and MdJAZ2, interact with MdBIM1 to integrate BR and JA signaling under cold stress. The E3 ubiquitin ligase MdATL73 targets MdBIM1 for degradation, reducing cold tolerance. This study reveals the crosstalk between BR and JA signaling and provides insights into the posttranslational regulation of BR signaling.
Article
Plant Sciences
Shuli Chai, Junhua Chen, Xiaolan Yue, Chenlin Li, Qiang Zhang, Victor Resco de Dios, Yinan Yao, Wenrong Tan
Summary: Brassinosteriod (BR) plays important roles in the regulation of plant growth, development, and environmental responses. The study reveals that BES1 and LBD37 antagonistically regulate the root foraging response under low nitrogen conditions. BES1 positively regulates key genes required for nitrate uptake and signaling, while negatively regulating repressors of the nitrate signaling pathway.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Cho-Rong Park, Van Tinh Nguyen, Ji-Hee Min, Hyunkyu Sang, Gah-Hyun Lim, Cheol Soo Kim
Summary: This study identified GmBEH3L1 in soybean as a negative regulator in ABA and dehydration responses through BR signaling.
Article
Biochemistry & Molecular Biology
Wenqi Feng, Yuan Liu, Yang Cao, Yiran Zhao, Hongwanjun Zhang, Fuai Sun, Qingqing Yang, Wanchen Li, Yanli Lu, Xuecai Zhang, Fengling Fu, Haoqiang Yu
Summary: This study reveals that ZmBES1/BZR1-3 and ZmBES1/BZR1-9 negatively regulate drought tolerance through different pathways in transgenic Arabidopsis, providing insights into the function of BES1/BZR1s in crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qunwei Bai, Lihaitian Wang, Shengdi Huang, Khawar Ali, Guishuang Li, Hongyan Ren, Bowen Zheng
Summary: Plants have receptor kinase proteins that mediate communication between cells and the environment, playing crucial roles in growth, development, and stress resistance. The receptor kinases EMS1 and BRI1 regulate different biological processes but interact in the downstream signaling pathways. This study found that EMS1 and BRI1 cooperatively regulate filament elongation through their transcription factors BES1/BZR1.
Article
Cell Biology
Liping Zhu, Huiqin Wang, Jiaojie Zhu, Xiaosi Wang, Bin Jiang, Liyong Hou, Guanghui Xiao
Summary: Continuous plant growth relies on cell division and elongation, which is regulated by brassinosteroids (BR). In this study, the researchers used cotton fiber to investigate cell-specific BR signaling and found that the transcriptional activator GhBES1 promotes fiber elongation by activating GhCERP, a protein related to cell elongation. GhCERP then transmits the BR signal to its target gene GhEXPA3-1, which ultimately promotes fiber cell elongation. Furthermore, the BR-mediated BES1-CERP-EXPA3 signaling cascade also promotes root and hypocotyl growth in Arabidopsis, indicating its broad significance in regulating BR-promoted cell elongation.
Article
Plant Sciences
Xiaofang Liu, Caide Zhao, Yuqi Gao, Yao Xu, Shujin Wang, Chaoshuo Li, Yinpeng Xie, Pengxiang Chen, Peizhi Yang, Li Yuan, Xiaofeng Wang, Lili Huang, Fengwang Ma, Hao Feng, Qingmei Guan
Summary: Our study reveals the multifaceted regulation of MdBES1 on MdMYB88 in BR biosynthesis and stress tolerance.
Article
Plant Sciences
HaoQiang Yu, Muhammad Hayder Bin Khalid, FengZhong Lu, FuAi Sun, JingTao Qu, BingLiang Liu, WanChen Li, FengLing Fu
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2019)
Article
Biochemistry & Molecular Biology
Muhammad Hayder Bin Khalid, Muhammad Ali Raza, Hao Qiang Yu, Imran Khan, Fu Ai Sun, Ling Yang Feng, Jing Tao Qu, Feng Ling Fu, Wan Chen Li
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Fuai Sun, Haoqiang Yu, Jingtao Qu, Yang Cao, Lei Ding, Wenqi Feng, Muhammad Hayder Bin Khalid, Wanchen Li, Fengling Fu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Fuai Sun, Lei Ding, Wenqi Feng, Yang Cao, Fengzhong Lu, Qingqing Yang, Wanchen Li, Yanli Lu, Nitzan Shabek, Fengling Fu, Haoqiang Yu
Summary: The study reveals that ZmBES1/BZR1-5 positively regulates kernel size in maize by interacting with specific genes and binding to their promoters. Multiple experiments provide insights into the molecular mechanisms of kernel development in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Yueming Tang, Fengzhong Lu, Wenqi Feng, Yuan Liu, Yang Cao, Wanchen Li, Fengling Fu, Haoqiang Yu
Summary: Sucrose non-fermenting-1 (SNF1)-related protein kinase 2 (SnRK2s) are plant-specific serine/threonine protein kinases that play crucial roles in the abscisic acid signaling pathway and abiotic stress response. The study identified 11 AnSnRK2 genes in the genome of Ammopiptanthus nanus and analyzed their expression profiles under osmotic stresses, showing that most AnSnRK2s were induced by NaCl and PEG-6000 treatments, indicating their key roles in stress tolerance. This research provides valuable insights into the function of AnSnRK2s.
Article
Biochemistry & Molecular Biology
Wenqi Feng, Yuan Liu, Yang Cao, Yiran Zhao, Hongwanjun Zhang, Fuai Sun, Qingqing Yang, Wanchen Li, Yanli Lu, Xuecai Zhang, Fengling Fu, Haoqiang Yu
Summary: This study reveals that ZmBES1/BZR1-3 and ZmBES1/BZR1-9 negatively regulate drought tolerance through different pathways in transgenic Arabidopsis, providing insights into the function of BES1/BZR1s in crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Bingliang Liu, Haoqiang Yu, Qinyu Yang, Lei Ding, Fuai Sun, Jingtao Qu, Wenqi Feng, Qingqing Yang, Wanchen Li, Fengling Fu
Summary: The study identified ZmLAZ1-4 as a novel zinc transporter on plasma and vacuolar membrane, which transports zinc under negative regulation of the ZmBES1/BZR1-11 transcription factor, providing insights into further understanding of the mechanism of ZmLAZ1-4 regulating zinc homeostasis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Fengzhong Lu, Wanchen Li, Yalin Peng, Yang Cao, Jingtao Qu, Fuai Sun, Qingqing Yang, Yanli Lu, Xuehai Zhang, Lanjie Zheng, Fengling Fu, Haoqiang Yu
Summary: This study characterized a maize PP2C family member, ZmPP2C26, which negatively regulated drought tolerance in maize by dephosphorylating ZmMAPK3 and ZmMAPK7. Alternative splicing generated ZmPP2C26L and ZmPP2C26S isoforms, with ZmPP2C26S showing higher phosphatase activity. The expression of ZmPP2C26L and ZmPP2C26 was inhibited by drought stress, and the zmpp2c26 mutant exhibited enhanced drought tolerance. However, overexpression of ZmPP2C26L and ZmPP2C26S decreased drought tolerance in Arabidopsis and rice. Phosphoproteomic analysis revealed that ZmPP2C26 also altered phosphorylation levels of proteins involved in photosynthesis. This study provides insights into the mechanism of PP2C in response to abiotic stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Fuai Sun, Malathy Palayam, Nitzan Shabek
Summary: Plant beta-amylase proteins play crucial roles in plant growth, development, stress response, and hormone regulation. This study reveals the noncatalytic adaptation of BZR1-type beta-amylases and provides insights into their regulatory functions as putative metabolic sensors in plants.
JOURNAL OF STRUCTURAL BIOLOGY
(2022)
Article
Agronomy
Yuanyuan Zhang, Yang Cao, Hongying Zheng, Wenqi Feng, Jingtao Qu, Fengling Fu, Wanchen Li, Haoqiang Yu
Summary: The study demonstrated the potential of the antifreeze protein from the super-xerophyte Ammopiptanthus nanus to improve chilling tolerance in maize. By successfully transferring the AnAFP gene into a parent line of commercial maize hybrids, transgenic lines with significantly improved phenotype were produced, showing the importance of this research for maize production.
Article
Ecology
M. H. B. Khalid, M. A. Raza, H. Q. Yu, F. A. Sun, Y. Y. Zhang, F. Z. Lu, L. Si, N. Iqbal, I Khan, F. L. Fu, W. C. Li
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
(2019)
