Article
Plant Sciences
Xing Ma, Paul Stodghill, Miao Gao, Keith L. Perry, Bryan Swingle
Summary: This study identified and characterized pathogenic SRP bacteria from potato samples collected in New York State in 2017. The findings revealed a variety of SRP species causing blackleg disease, including the first report of P. versatile causing this disease in New York State. The phylogenetic relationships between the SRP strains were determined using genomic sequence comparison.
Article
Microbiology
Tongling Ge, Fatemeh Ekbataniamiri, Steven B. Johnson, Robert P. Larkin, Jianjun Hao
Summary: This study examined the presence of Dickeya dianthicola and P. parmentieri in potato samples collected in Northeastern USA, and conducted field trials to investigate their interaction and disease severity. The results showed that D. dianthicola was more virulent than P. parmentieri, but co-inoculation of both species led to increased disease severity compared to single-species inoculation.
Article
Microbiology
Rebecca D. Curland, Amanda Mainello, Keith L. Perry, Jianjun Hao, Amy O. Charkowski, Carolee T. Bull, Ryan R. McNally, Steven B. Johnson, Noah Rosenzweig, Gary A. Secor, Robert P. Larkin, Beth K. Gugino, Carol A. Ishimaru
Summary: An outbreak of bacterial soft rot and blackleg of potato has been occurring in the northeastern region of the United States since 2014. A phylogenetic study was conducted to identify the pathogens involved in the epidemic, revealing a variety of species clustering with certain type strains. The study also found low genetic diversity in D. dianthicola strains and greater diversity in P. parmentieri strains among those isolated from symptomatic potato samples in 2015-2016.
Article
Microbiology
Patrice de Werra, Christophe Debonneville, Isabelle Kellenberger, Brice Dupuis
Summary: Pectobacterium and Dickeya species are causal agents of blackleg and soft rot diseases in potatoes. P. brasiliense has shown a drastic increase in abundance in most European countries in the past decade, despite not necessarily being the most virulent. Pathogenicity alone does not explain the prevalence of certain species in Swiss potato fields.
Article
Microbiology
Dorota Soltys-Kalina, Anna Grupa-Urbanska, Renata Lebecka, Maud Tallant, Isabelle Kellenberger, Brice Dupuis
Summary: This article explores the possibility of using glycoalkaloids (GAs) produced by potato tubers after the greening process as a control method for blackleg disease caused by Dickeya and Pectobacterium species. The study found that GAs extracted from four potato cultivars exhibited bacteriostatic and bactericidal effects against both bacteria strains in growth media. Field experiments showed that plants grown from green seed tubers had lower incidence of blackleg, except for one-year trial. However, the control effect was marginal, possibly due to low production of GAs by cv. Agria tubers after greening.
Article
Horticulture
Eva Sanchez-Hernandez, Vicente Gonzalez-Garcia, Jesus Martin-Gil, Belen Lorenzo-Vidal, Ana Palacio-Bielsa, Pablo Martin-Ramos
Summary: The yew tree is known for its longevity and regeneration, and despite being poisonous, it has a long history of medicinal use. This study used GC-MS chemical profiling to identify bioactive compounds in the aqueous ammonia/hydromethanolic extracts of various parts of the yew tree. The extracts showed antimicrobial activity against bacterial pathogens responsible for potato diseases, and could potentially be used in integrated pest management.
Article
Microbiology
Marta Loc, Dragana Milosevic, Zarko Ivanovic, Maja Ignjatov, Dragana Budakov, Jovana Grahovac, Mila Grahovac
Summary: This study confirms the presence of Pectobacterium brasiliense, Pectobacterium punjabense, and Pectobacterium carotovorum species in samples collected from the Province of Vojvodina in Serbia. It also confirms the distinctiveness of the newly described P. punjabense in Serbia.
Article
Plant Sciences
Wanxin Han, Jinhui Wang, Minna Pirhonen, Yang Pan, Jingxin Qin, Shangqing Zhang, Jiehua Zhu, Zhihui Yang
Summary: Blackleg and aerial stem rot of potato caused by soft rot enterobacteria have increased in Hebei Province, China. In this study, bacterial pathogens were identified and characterized in potato plants showing blackleg or aerial stem rot. Pectobacterium atrosepticum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Pectobacterium parmentieri were identified, along with a new species of Pectobacterium polonicum. Isolate BY21311 of P. polonicum showed reduced ability to macerate potato tubers compared to other Pectobacterium species.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Yeshitila Degefu
Summary: Recent methodological developments have revealed the etiological diversity of potato blackleg and soft rot Pectobacteriaceae, with at least five bacterial species confirmed to cause blackleg in potatoes in Finland. Molecular detection data collected over 14 years in Finland showed that single species infection is more common than multiple colonization, and simultaneous occurrences of different Pectobacterium strains are more frequent than between Dickeya and Pectobacterium species. The absence of co-occurrence of Dickeya solani and Pectobacterium atrosepticum is intriguing.
AGRICULTURAL AND FOOD SCIENCE
(2021)
Article
Microbiology
Zhenhe Su, Xiaomeng Liu, Qinggang Guo, Lifeng Xuan, Xiuyun Lu, Lihong Dong, Xiaoyun Zhang, Peipei Wang, Weisong Zhao, Yuanhang Qu, Shezeng Li, Ping Ma
Summary: In this study, the pathogenicity of two pectinolytic strains of Pectobacterium spp. (P. brasiliense and P. carotovorum) was investigated. The results showed that P. carotovorum had higher cell wall-degrading enzyme activities and caused more severe disease symptoms in potato tubers compared to P. brasiliense. The pathogenicity of both strains increased with temperature. Furthermore, coinoculation of P. carotovorum with P. brasiliense resulted in more severe pathogenicity, especially when P. carotovorum had a bacterial number advantage at the beginning. These findings provide new insights into the pathogenicity caused by mixed infections of different Pectobacterium spp.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Jacques Pedron, Santiago Schaerer, Isabelle Kellenberger, Frederique Van Gijsegem
Summary: Potato blackleg and soft rot caused by Pectobacterium and Dickeya are devastating bacterial diseases globally. In Europe, Pectobacterium spp. were historically the main causative agents, but in Switzerland, there was a higher occurrence of Dickeya species, particularly in the 1980s. The genomic comparison of early D. solani isolates from the 1990s with strains from the 2000s outbreak in Europe showed minimal differences, suggesting no major changes in virulence genes over time.
Article
Agronomy
Leah Tsror (Lahkim), Valerie Helias, Sara Mordechai-Lebiush, Orly Erlich, Marina Hazanovsky, Laura Chalupowicz, Michal Reuven, Orit Dror, Lea Valinsky, Angelique Laurent, Shulamit Manulis-Sasson
Summary: The study characterized the genetic diversity and virulence variability of 68 Pbr strains isolated from different sources in Israel, Europe, and France. Analysis revealed that the tested Pbr strains are not a homogeneous group and the main source of Pbr in Israel may be seed tubers imported from Europe. This study provides insights into the biodiversity and population structure of P. brasiliense under hot climate conditions.
Article
Multidisciplinary Sciences
Ryan Domingo, Cristian Perez, Diksha Klair, Huong Vu, Alika Candelario-Tochiki, Xupeng Wang, Amihan Camson, Jaclyn Nicole Uy, Mouauia Salameh, Dario Arizala, Shefali Dobhal, Gamze Boluk, Jon-Paul Bingham, Francisco Ochoa-Corona, Md Emran Ali, James P. Stack, Jacqueline Fletcher, Jenee Odani, Daniel Jenkins, Anne M. Alvarez, Mohammad Arif
Summary: A reliable, rapid, field-deployable loop-mediated isothermal amplification (LAMP) assay for specific detection of P. parmentieri has been developed, showing promising results in both laboratory and field tests.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Sanja Markovic, Slavisa Stankovic, Aleksandra Jelusic, Renata Ilicic, Andrea Kosovac, Dobrivoj Postic, Tatjana Popovic
Summary: Blackleg outbreaks were observed in three fields in Serbia for two consecutive years, leading to a decrease in plant yield. Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola were identified as causative agents, with genetic characterization revealing multiple genotypes. Multiple genetic profiles and a new D. dianthicola genotype were discovered through MLSA analysis in Serbia.
Review
Microbiology
Yvann Bourigault, Andrea Chane, Corinne Barbey, Sylwia Jafra, Robert Czajkowski, Xavier Latour
Summary: Promoter-probe vectors carrying fluorescent protein-reporter genes are powerful tools for studying microbial ecology, epidemiology, and etiology. Studies using these vectors in potato plants have provided insights into bacterial activities leading to tuber softening and disease symptoms. The use of dual-colored reporters has allowed for simultaneous visualization of bacterial spread and activity, shedding light on the chronology of events related to tuber maceration and quorum-sensing communication disrupted by biocontrol agents.
Article
Plant Sciences
Felicia J. Setiono, Debotri Chatterjee, Marc Fuchs, Keith L. Perry, Jeremy R. Thompson
Article
Plant Sciences
Elizabeth Cieniewicz, Jeremy R. Thompson, Heather McLane, Keith L. Perry, Gerald S. Dangl, Quinlan Corbett, Timothy Martinson, Alice Wise, Anna Wallis, James O'Connell, Rick Dunst, Kerik Cox, Marc Fuchs
Article
Plant Sciences
Xing Ma, Paul Stodghill, Miao Gao, Keith L. Perry, Bryan Swingle
Summary: This study identified and characterized pathogenic SRP bacteria from potato samples collected in New York State in 2017. The findings revealed a variety of SRP species causing blackleg disease, including the first report of P. versatile causing this disease in New York State. The phylogenetic relationships between the SRP strains were determined using genomic sequence comparison.
Article
Plant Sciences
Rongbin Hu, Nayma P. Dias, Nourolah Soltani, Jose Vargas-Asencio, Darrell D. Hensley, Keith L. Perry, Leslie L. Domier, M. R. Hajimorad
Summary: The study conducted a survey of viruses and viroids in both cultivated and wild grapevines, detecting multiple viruses and viroids in both populations. Differences in viral sequences between cultivated and wild grapevines suggest limited virus movement between the two populations.
Article
Plant Sciences
Jeremy R. Thompson, Annika L. Gomez, Aisha Younas, Juliana Gonzalez-Tobon, Alex Cha, Keith L. Perry
Summary: This study surveyed virus populations in free-living grapevines in the Finger Lakes region of New York State, showing a high incidence of grapevine asteroid mosaic-associated virus (GAMaV) infection. Commercially grown Vitis vinifera plants in the same region were also found to be infected with different viruses, highlighting the potential exchange of microbiota between cultivated and uncultivated grapevines.
Editorial Material
Plant Sciences
Keith L. Perry
Editorial Material
Plant Sciences
Xing Ma, Jessie Brazil, Hannah Rivedal, Keith L. Perry, Ken Frost, Bryan Swingle
Article
Plant Sciences
Xing Ma, Keith L. L. Perry, Bryan Swingle
Summary: In this study, a recent strain of Clavibacter sepedonicus, K496, was isolated from potato tubers with vascular cylinder or pith tissue discolorations. The genome sequence of K496 was de novo assembled using Nanopore reads and polished with Illumina reads. The resulting K496 genome consists of a circular chromosome of 3,266,016 bp and a linear plasmid of 135,489 bp. Bioinformatics analysis predicted a total of 3,301 genes in the K496 genome, including protein-coding genes, pseudogenes, rRNA-coding genes, tRNAs, and noncoding RNAs.
Article
Virology
Keith L. Perry, Heather McLane, Jeremy R. Thompson, Marc Fuchs
ARCHIVES OF VIROLOGY
(2018)