Journal
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 20, Issue 13, Pages 8881-8886Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cp08478a
Keywords
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Funding
- National Basic Research Program of China [2015CB932004]
- National Natural Science Foundation of China [31671012]
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A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I-1574/I-1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.
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