Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Article
Chemistry, Multidisciplinary
Sun Young Park, Woo Chang Song, Beomjin Kim, Jin-Woo Oh, Geuntae Park
Summary: Nano-GO induces EMT in human RPE cells by regulating phospholipase D signaling, enhancing cell migration ability and collagen gel contraction, leading to intracellular ROS generation. This study provides evidence of adverse health effects of Nano-GO on RPE cells.
Article
Biochemistry & Molecular Biology
Geon-Ho Lee, Jayeon Cheon, Donghee Kim, Hee-Sook Jun
Summary: LPA promotes EMT and renal tubular fibrosis through LPAR1 signaling, downregulating E-cadherin and upregulating vimentin expression. This effect is mediated by regulation of KLF5 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Minghan Guan, Yifeng Huang, Xiaowen Lin
Summary: This study found that sufentanil can inhibit the proliferation, migration, invasion, and epithelial-mesenchymal transition of lung cancer cells by regulating the Wnt/beta-catenin signaling pathway.
Article
Biochemistry & Molecular Biology
An-nan Zhang, Nan Li, Zhuo-chang Chen, Ya-li Guo, Cui-jie Tian, Dong-jun Cheng, Xue-yi Tang, Xiao-yu Zhang
Summary: Objective of this study was to investigate the effect of amygdalin, an active ingredient in bitter almonds, on TGF-beta-induced EMT in bronchial epithelial cells and elucidate the underlying mechanisms. The results showed that amygdalin treatment relieved airway wall remodeling and decreased the expressions of EMT markers. Furthermore, amygdalin inhibited TGF-beta-induced EMT through regulating the expression of HPSE.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Environmental Sciences
Tianzhu Sun, Dengyang Zhang, Zehao Wang, Bingyu Zhao, Yaping Li, Xiuli Sun, Jia Liu, Xuanjun Wang, Jun Sheng
Summary: Studies have shown that cervical cancer cells develop an EMT phenotype partly through activation of the Notch1 signaling pathway, leading to resistance to paclitaxel. Targeting the Notch1 signaling pathway may be a strategy to reverse the EMT phenotype and increase sensitivity of cervical cancer cells to paclitaxel treatment.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Zhiwen Xie, Yongqing Zhang, Wenlan Sun, Shan Hua, Bangmin Han, Juntao Jiang, Yingjian Zhu, Yifeng Jing
Summary: Maternal exposure to dibutyl phthalate (DBP) induces renal fibrosis in offspring. The study investigated the role of connexin 43 (Cx43) in DBP-induced renal fibrosis. The results showed that DBP increased the expression of Cx43 in renal tubular epithelial cells (RTECs), and silencing Cx43 alleviated DBP-induced epithelial-mesenchymal transition (EMT) in HK-2 cells. Bioinformatics analysis revealed that DBP upregulated Cx43 expression through the Ang II/AMPK alpha 2 signaling pathway. These findings suggest that targeting Cx43 and the Ang II/AMPK alpha 2 pathway may be a potential treatment for renal fibrosis.
Review
Oncology
Xiaobo Zheng, Fuzhen Dai, Lei Feng, Hong Zou, Li Feng, Mingqing Xu
Summary: The interplay between epithelial-mesenchymal plasticity and cancer stem cells plays a crucial role in cancer progression, affecting the aggressiveness of tumor cells and their response to treatment. Understanding the dynamic nature of EMT as a hybrid state rather than a binary model is important for unraveling the complexities of cancer biology and developing targeted therapeutic interventions.
FRONTIERS IN ONCOLOGY
(2021)
Article
Pharmacology & Pharmacy
Lu Yu, Hyun Ji Kim, Mi Kyung Park, Hyun Jung Byun, Eun Ji Kim, Boram Kim, Minh Tuan Nguyen, Ji Hyun Kim, Gyeoung Jin Kang, Ho Lee, Soo Youl Kim, Seung Bae Rho, Chang Hoon Lee
Summary: This study revealed that Ethacrynic acid (ECA) inhibits the EMT induced by SPC or TGF-β1 in lung cancer cells, potentially through downregulating NDP expression and inhibiting WNT activation. ECA could be a new drug candidate for lung cancer treatment.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Caihong Liu, Junjuan Lu, Ting Yuan, Lihua Xie, Li Zhang
Summary: This study aimed to investigate whether exosomes can influence the ferroptosis of bronchial epithelial cells (BECs) in chronic obstructive pulmonary disease (COPD) and the mechanisms involved. The researchers extracted and identified endothelial progenitor cells (EPCs) and EPC-Exo from blood samples of normal subjects and COPD patients, and established a COPD animal model. They found that EPCs could alleviate cigarette smoke-induced ferroptosis in BECs by transporting exosomes. In addition, exosomes also alleviated cigarette smoke-induced ferroptosis and airway remodeling in mice. The study revealed that the PTGS2/PGE2 pathway and miR-26a-5p played important roles in the regulation of ferroptosis and epithelial-mesenchymal transition in BECs.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Joseph L. Mertz, Srinivasa R. Sripathi, Xue Yang, Lijun Chen, Noriko Esumi, Hui Zhang, Donald J. Zack
Summary: Studying kinase/phosphatase signaling cascades in induced EMT of RPE can provide insights into the pathways mediating this process, with liver hyperplasia and HGF-MET signaling showing the highest enrichment. HGF and epidermal growth factor signaling pathways, inhibited by EMT induction, play a role in regulating the RPE transcriptional profile.
Article
Medicine, General & Internal
Wei Liu, Gaobo Huang, Yang Yang, Ruixia Gao, Shaoqiang Zhang, Bo Kou
Summary: The study demonstrated that oridonin inhibits migration and invasion of nasopharyngeal carcinoma cells through negatively regulating the AKT/STAT3 signaling pathway, suggesting that AKT/STAT3 signaling may be a potential therapeutic target for oridonin against nasopharyngeal carcinoma.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Cell Biology
Lin Liao, Lianxiang Duan, Yue Guo, Baojuan Zhou, Qiming Xu, Chuanfu Zhang, Weiwei Liu, Wenrui Liu, Ziyang Liu, Jing Hu, Jie Chen, Jianrao Lu
Summary: Hypoxia stimulates the expression of TRIM46 in renal tubular cells, leading to the induction of epithelial-mesenchymal transition (EMT) and renal fibrosis. The upregulation of TRIM46 under hypoxia enhances the expression of mesenchymal markers and reduces the expression of epithelial markers, accompanied by an increase in nuclear beta-catenin. TRIM46 promotes the ubiquitination and degradation of Axin1, a negative regulator of Wnt/beta-catenin signaling. In a rat renal fibrosis model, increased TRIM46 and decreased Axin1 levels were observed. These findings suggest a novel mechanism for hypoxia-induced renal fibrosis through the EMT process mediated by TRIM46.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shuai Yang, Hui Li, Haipei Yao, Yao Zhang, Huiqian Bao, Liangjing Wu, Conghui Zhang, Min Li, Le Feng, Jingfa Zhang, Zhi Zheng, Guotong Xu, Fang Wang
Summary: The study revealed that lncRNA ERLR plays a role in TGF-beta 1-induced EMT in human RPE cells and is involved in the pathogenesis of PVR, providing new insights into the mechanism and treatment of the disease.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Min Cheng, Xiaolin Ye, Jiemin Dai, Feiji Sun
Summary: The study revealed that SOS1 may induce EMT in EOC cells with high metastatic potential, through activation of the NF-KB pathway and the transcriptional activity of Snail, ultimately regulating cell motility.
TRANSLATIONAL ONCOLOGY
(2021)
Editorial Material
Physiology
Isotta Chimenti, Susanne Sattler, Gonzalo del Monte-Nieto, Elvira Forte
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Isotta Chimenti, Vittorio Picchio, Francesca Pagano, Leonardo Schirone, Sonia Schiavon, Luca D'Ambrosio, Valentina Valenti, Maurizio Forte, Flavio di Nonno, Speranza Rubattu, Mariangela Peruzzi, Francesco Versaci, Ernesto Greco, Antonella Calogero, Elena De Falco, Giacomo Frati, Sebastiano Sciarretta
Summary: This study evaluated the impact of autophagy modulation on the viability and phenotype of murine cardiac stromal cells (CSCs) under different stress conditions. The results showed that autophagy induction preserved CSC viability and function, suggesting its potential in cardiac repair mechanisms.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Antonella Bordin, Maila Chirivi, Francesca Pagano, Marika Milan, Marco Iuliano, Eleonora Scaccia, Orazio Fortunato, Giorgio Mangino, Xhulio Dhori, Elisabetta De Marinis, Alessandra D'Amico, Selenia Miglietta, Vittorio Picchio, Roberto Rizzi, Giovanna Romeo, Fabio Pulcinelli, Isotta Chimenti, Giacomo Frati, Elena De Falco
Summary: Platelet lysate (PL) contains a subset of small vesicles with angiogenic effects, enriched with miR-126. These extracellular vesicles (EVs) play a key role in promoting angiogenic tubular structures in endothelial cells, representing a potential target for developing a product solely made of EVs for biological manipulation.
CELL PROLIFERATION
(2022)
Article
Cardiac & Cardiovascular Systems
Gloria Garoffolo, Manuel Casaburo, Francesco Amadeo, Massimo Salvi, Giacomo Bernava, Luca Piacentini, Isotta Chimenti, Germana Zaccagnini, Gesmi Milcovich, Estella Zuccolo, Marco Agrifoglio, Sara Ragazzini, Otgon Baasansuren, Claudia Cozzolino, Mattia Chiesa, Silvia Ferrari, Dario Carbonaro, Rosaria Santoro, Martina Manzoni, Loredana Casalis, Angela Raucci, Filippo Molinari, Lorenzo Menicanti, Francesca Pagano, Toshiro Ohashi, Fabio Martelli, Diana Massai, Gualtiero I. Colombo, Elisa Messina, Umberto Morbiducci, Maurizio Pesce
Summary: This study suggests that preventing molecular translation of mechanical cues in cardiac stromal cells can reduce the impact of cardiac maladaptive remodeling and have a positive effect on fibrosis.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Veronica De Paolis, Fabio Maiullari, Maila Chirivi, Marika Milan, Chiara Cordiglieri, Francesca Pagano, Alessandra Rita La Manna, Elena De Falco, Claudia Bearzi, Roberto Rizzi, Chiara Parisi
Summary: The cellular heterogeneity in the tumor environment of breast cancer is complex, involving various cell types with different functions. The physical properties of the tumor extracellular matrix play a crucial role in protecting the tumor niche. TAMs with an immunosuppressive phenotype are important for establishing a tumor phenotype that evades the immune system. NF-kappa B transcription factors have a connection between inflammation and cancer. In TAMs, an unusual association of NF-kappa B components affects the stiffness of the tumor extracellular matrix.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Francesca Pagano, Vittorio Picchio, Antonella Bordin, Elena Cavarretta, Cristina Nocella, Claudia Cozzolino, Erica Floris, Francesco Angelini, Alessia Sordano, Mariangela Peruzzi, Fabio Miraldi, Giuseppe Biondi-Zoccai, Elena De Falco, Roberto Carnevale, Sebastiano Sciarretta, Giacomo Frati, Isotta Chimenti
Summary: Cardiac stromal cells (CSCs) play a major role in fibrosis, with dysmetabolic conditions having a strong impact on cardiac fibrosis. This study aimed to characterize CSCs from dysmetabolic patients and explore the modulation of oxidative state and autophagy to mitigate fibrotic commitment. The results showed that appropriate modulation of autophagy and oxidative state in CSCs can restore their features, providing a potential novel strategy for treating cardiac fibrosis in dysmetabolic patients.
JOURNAL OF PATHOLOGY
(2022)
Editorial Material
Cell & Tissue Engineering
Claudia Cozzolino, Vittorio Picchio, Erica Floris, Francesca Pagano, Wael Saade, Mariangela Peruzzi, Giacomo Frati, Isotta Chimenti
Summary: Smoking habits have a significant impact on cardiovascular health by damaging differentiated and functional cells, as well as impairing reparative mechanisms. The introduction of modified tobacco risk products (MRPs) as alternative smoking devices has raised concerns regarding their effects on cardiovascular repair and regenerative cells. This review critically evaluates the limited available data on the molecular and cellular effects of MRPs on cardiovascular repair and regeneration.
CURRENT STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Leonardo Schirone, Lorenzo Loffredo, Roberto Carnevale, Simona Battaglia, Roberta Marti, Stefano Pizzolo, Simona Bartimoccia, Cristina Nocella, Vittoria Cammisotto, Wael Saade, Alessandra Tanzilli, Sebastiano Sciarretta, Isotta Chimenti, Elena De Falco, Elena Cavarretta, Vittorio Picchio, Mariangela Peruzzi, Antonino Marullo, Fabio Miraldi, Francesco Violi, Andrea Morelli, Giuseppe Biondi-Zoccai, Giacomo Frati
Summary: Although heat-not-burn cigarettes (HNBCs) are promoted as healthier alternatives to traditional combustion cigarettes, this study found that HNBCs have similar detrimental effects on cardiovascular health in both men and women, causing oxidative stress and platelet activation.
Review
Biochemistry & Molecular Biology
Vittorio Picchio, Erica Floris, Yuriy Derevyanchuk, Claudia Cozzolino, Elisa Messina, Francesca Pagano, Isotta Chimenti, Roberto Gaetani
Summary: Ex vivo modelling systems are important in cardiovascular research for reducing lab animal use and advancing personalized medicine. Integrating multiple cell types in complex setups simulates the intercellular communication of the microenvironment. 3D culture systems enhance the physiological significance of these models. Various multicellular 3D models, such as microtissues, bioprinted constructs, engineered tissues, and organs-on-chip, have been used to study cardiac fibrosis, leading to important discoveries and potential therapeutic molecules. Future developments will further enhance the translational impact of these models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maria Vincenza Chiantore, Marco Iuliano, Roberta Maria Mongiovi, Fabiola Luzi, Giorgio Mangino, Lorenzo Grimaldi, Luisa Accardi, Gianna Fiorucci, Giovanna Romeo, Paola Di Bonito
Summary: Actinic keratosis (AK) is a precursor to cutaneous squamous cell carcinoma (cSCC) and is highly prevalent in the elderly population, particularly among those with sun-exposed skin. This study identified 43 dysregulated microRNAs (miRNAs) in AK samples and validated the expression of several key miRNAs. Pathway analysis revealed that these miRNAs targeted pathways involved in tumorigenesis, highlighting the importance of miRNA regulation in keratinocyte cancer.
Article
Immunology
Lorena Capriotti, Marco Iuliano, Roberto Lande, Loredana Frasca, Mario Falchi, Paolo Rosa, Giorgio Mangino, Giovanna Romeo
Summary: This study investigates the role of extracellular vesicles (EVs) in psoriasis. It found that psoriasis-related cytokines can affect the release and cargo of EVs. Interestingly, EVs derived from cytokine-treated cells can induce Netosis in neutrophils and upregulate the expression of certain genes in recipient cells. These findings highlight the involvement of EVs in the development and spread of psoriasis.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Review
Oncology
Vittorio Picchio, Antonella Bordin, Erica Floris, Claudia Cozzolino, Xhulio Dhori, Mariangela Peruzzi, Giacomo Frati, Elena De Falco, Francesca Pagano, Isotta Chimenti
Summary: Cardiac stromal cells play important roles in the homeostasis and repair of the heart. Recent studies have shown that their transcriptional profiles are dynamically regulated and associated with the cardiac response to injury. This review outlines the phenotypical and molecular markers, as well as the approaches for identifying and classifying cardiac stromal cells.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Article
Biology
Vittorio Picchio, Francesca Pagano, Isotta Chimenti
Summary: This article discusses the evolution of definitions of cardiac stromal cells and their significant role in cardiac repair and regeneration.