Journal
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
Volume 470, Issue 9, Pages 1325-1333Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00424-018-2154-8
Keywords
ATP-sensitive K+ channels; Umbilical artery; Gestational diabetes mellitus; Protein kinase A
Categories
Funding
- National Research Foundation of Korea (NRF) - Korea government (Ministry of Education) [2016-R1D1A3B03930169, 2017-R1D1A1B03028467)]
- National Research Foundation of Korea (NRF) - Korea government (Ministry of Science, ICT and Future Planning) [2015-R1A4A1038666]
Ask authors/readers for more resources
We investigated the alterations of ATP-sensitive K+ (K-ATP) channels in human umbilical arterial smooth muscle cells during gestational diabetes mellitus (GDM). The amplitude of the K-ATP current induced by application of the K-ATP channel opener pinacidil (10 mu M) was reduced in the GDM group than in the control group. Pinacidil-induced vasorelaxation was also predominant in the normal group compared with the GDM group. Reverse transcription polymerase chain reaction and Western blot analysis suggested that the expression of K-ATP channel subunits such as Kir6.1, Kir6.2, and SUR2B were decreased in the GDM group relative to the normal group. The application of forskolin and adenosine, which activates protein kinase A (PKA) and thereby K-ATP channels, elicited K-ATP current in both the normal and GDM groups. However, the current amplitudes were not different between the normal and GDM groups. In addition, the expression levels of PKA subunits were not altered between the two groups. These results suggest that the reduction of K-ATP current and K-ATP channel-induced vasorelaxation are due to the decreased expression of K-ATP channels, not to the impairment of K-ATP-related signaling pathways.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available