Article
Agronomy
Zhenzhen Zhao, Lan Zhang, Yanning Zhang, Liangang Mao, Lizhen Zhu, Xingang Liu, Hongyun Jiang
Summary: The study investigated the enzymatic response of cytochrome P450 monooxygenase (CYP) and glutathione S-transferase (GST) genes after CPT treatment in the fat body cells of Spodoptera exigua. The effects of CPT on the growth and development of S. exigua larvae were also examined. The relative expression levels of selected CYP and GST genes changed significantly during the CPT treatment period, suggesting their involvement in CPT metabolism.
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: The study identified and characterized a novel Spodoptera litura P450 gene, CYP321B1, which plays a crucial role in tannin detoxification and metabolism in insects. By conducting RNA interference and metabolome analysis, it was confirmed that silencing CYP321B1 significantly affects weight gain under tannin induction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Multidisciplinary
Bi-Yun Zhang, Feng-Qi Li, Cheng Qu, Youssef Dewer, Yue-Jun Fu, Chen Luo
Summary: In this study, researchers identified genes related to sex pheromone biosynthesis in the Noctuid moths Spodoptera litura and Spodoptera exigua. They found that only the genes SlitDes5 and SexiDes5 were significantly overexpressed in the sex pheromone glands of these moths. Functional analysis of the SlitDes5 gene showed that its expression level could be reduced by 49.42% through RNA interference. These findings contribute to a better understanding of the key genes involved in sex pheromone biosynthesis and provide potential gene targets for pest control strategies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: In this study, a P450 gene CYP6AB12 was identified and characterized from Spodoptera litura, an insect species. The gene was found to play an important role in the detoxification and metabolism of gossypol, a plant allelochemical. Silencing the gene resulted in lowered expression levels and increased sensitivity to gossypol. These findings confirm the broad detoxification and metabolism abilities of P450s and provide a molecular basis for further research on detoxification metabolism and pest control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Shuhua Liu, Xinge Yao, Xing Xiang, Qunfang Yang, Xuegui Wang, Tian Xin, Siyuan Yu
Summary: The study found that chlorantraniliprole resistance in Spodoptera exigua carries fitness costs, but these costs will disappear with the recovery of sensitivity when the insecticide is no longer used, supporting the idea that resistance can be managed by rotating with alternate insecticides.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Wen-Tao Hou, Christian Staehelin, Mohammed Esmail Abdalla Elzaki, Muhammad Hafeez, Yu-Sen Luo, Rui-Long Wang
Summary: Spodoptera litura is a widely distributed, highly polyphagous pest that has developed resistance to various insecticides. This study found that elevated cytochrome P450 monooxygenase enzyme activity, particularly the overexpression of the novel gene CYP6AE68, in populations with resistance to indoxacarb may play a significant role in detoxification processes.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Agronomy
Qi-Mei Tan, Wei-wei Chen, Hong-hong Li, Shu-cheng Liao, Guo-qiang Yi, Yong Mei, Jie Luo, Hui-hua Tan, Xue-Sheng Li
Summary: The adipokinetic hormone (AKH) plays a crucial role in enhancing chlorantraniliprole resistance in the fall armyworm (FAW). AKH positively influences the expression and content of P450 genes, thereby increasing FAW's survival against chlorantraniliprole. These findings provide valuable insights into the regulation of insecticide resistance and the development of control strategies for FAW.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Li Shi, Wenlin Li, Yating Dong, Yao Shi, Yuliang Zhou, Xiaolan Liao
Summary: This study identified two transcripts of Spodoptera litura CPR (SlCPR-X1 and SlCPR-X2) and found that SlCPR-X1 plays a major role in indoxacarb resistance in S. litura. The study highlights the importance of insect CPR in xenobiotics detoxification and resistance development, suggesting CPR as a potential target for insecticide resistance management through RNAi or CRISPR/Cas.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kang Wang, Junning Zhao, Zhaojun Han, Maohua Chen
Summary: This study conducted genome-wide transcriptional analysis on the lambda-cyhalothrin susceptible (SS) and resistant (LC-RR) strains of the bird-cherry-oat aphid, Rhopalosiphum padi. The results showed that the overexpression of two P450 genes (CYP6DC1 and CYP380C47) contributed to the lambda-cyhalothrin resistance in the pest. This study provides knowledge for further analyzing the molecular mechanism of resistance to pyrethroids in R. padi.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Genetics & Heredity
Qun Yang, Wanjun Yang, Feng Shang, Biyue Ding, Jinzhi Niu, Jinjun Wang
Summary: This study sequenced the transcriptome and small RNA of the invasive Asian tramp snail collected from citrus orchards in southern China. Genes associated with xenobiotic metabolism and core RNAi machinery were identified, providing a foundation for further molecular research on this species and other land snails with agricultural importance.
FRONTIERS IN GENETICS
(2021)
Article
Physiology
Hui-Lin Chen, Ali Hasnain, Qing-Hua Cheng, Li-Juan Xia, Yu-Hao Cai, Rong Hu, Chang-Wei Gong, Xue-Mei Liu, Jian Pu, Lei Zhang, Xue-Gui Wang
Summary: This study conducted resistance monitoring of 11 field-collected populations of the fall armyworm in Sichuan Province, China. The results showed variations in resistance levels to different insecticides among the populations. Overexpression of certain genes and resistance to multiple classes of insecticides may contribute to the development of resistance to chlorantraniliprole in the fall armyworm.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wenting Dai, Bin Zhu, Marcel van Tuinen, Tao Zhu, Dongliang Shang, Pedro Almeida, Pei Liang, Hidayat Ullah, Liping Ban
Summary: This study analyzed the genetic mechanisms underlying pesticide resistance in diamondback moths, and identified the CYP6B6-like gene as being involved in the resistance. The study also demonstrated that differential gene expression contributes to the development of resistance. The findings have important implications for the development of effective pest control measures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Tianfei Peng, Xuemei Liu, Fayi Tian, Hongfei Xu, Fengting Yang, Xuewei Chen, Xiwu Gao, Yuntong Lv, Jianyi Li, Yiou Pan, Qingli Shang
Summary: The study revealed that the upregulation of certain cytochrome P450 genes is associated with spirotetramat resistance in A. gossypii, and some lncRNAs may regulate the expression of these P450 genes to affect resistance. These findings provide a molecular basis for further investigating cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Han Wu, Dan Zhao, Xiao-Chang Guo, Zhao-Rui Liu, Rui-Jun Li, Xiu-Jun Lu, Wei Guo
Summary: Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects. Putative Group V CDAs were identified and characterized from beet armyworm larvae. The expression of these CDAs was found to be regulated by 20-hydroxyecdysone and juvenile hormone analog. Silencing of Group V CDAs resulted in compacted and evenly distributed intestinal wall cell layer, fragmented or disappeared vesicles, scarce PM structure, and loose and chaotic chitin microfilament structure. These results indicate the essential role of Group V CDAs in the growth and structuring of the midgut in beet armyworm larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Zhi Cheng, Da Wang, Shipeng Han, Cheng Zuo, Yunzhuan He
Summary: This study identified differentially expressed genes associated with thiamethoxam resistance in the seven-spot ladybird beetle through transcriptome sequencing. Up-regulation of certain metabolic enzymes and transport proteins, as well as down-regulation of nicotinic acetylcholine receptor gene, were observed in the resistant strain.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)