Journal
PATHOLOGY RESEARCH AND PRACTICE
Volume 214, Issue 5, Pages 776-783Publisher
ELSEVIER GMBH
DOI: 10.1016/j.prp.2018.02.002
Keywords
Non-small cell lung cancer; miR-1260b; PTPRK; Migration; Invasion
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Funding
- Natural Science Foundation of Zhejiang Province [LY16H160002]
- Public Technology Applied Research Program [2015GZ15]
- Public Applied Research Program [2016GY22, 2016GY30]
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Objective: Non-small cell lung cancer (NSCLC) accounts for 80-85% of lung cancer cases which cause most of cancer-related deaths globally. As our previous study discovered miR-1260b can be regarded as a specific signature for metastasis in NSCLC patients. However, the molecular mechanisms of miR-1260b underlying NSCLC progression and metastasis remain dismal. Methods: The expression of miR-1260b in NSCLC tissues and cell lines were examined by real-time PCR, the effects of miR-1260b on cell migration, invasion and proliferation were evaluated in vitro. Furthermore, luciferase reporter assay was performed to identify the targets of miR-1260b, and the association between miR1260b and its target gene was determined by real-time PCR and western blot assay. Results: The results showed that miR-1260b was significantly upregulated in NSCLC cell lines. The inhibition of miR-1260b expression decreased the migratory and invasive rates in A549 cells while miR-1260b overexpression had the opposite effect. Furthermore, PTPRK was identified as a direct target of miR-1260b, and PTPRK expression was inversely correlated with miR-1260b in NSCLC cell lines and clinical tissues. Conclusions: These results suggested that miR-1260b may play an important role in NSCLC metastasis progression and could serve as a putative target for diagnosis and treatment of NSCLC.
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