Article
Engineering, Environmental
Zexin Tao, Xiaojuan Lin, Yao Liu, Feng Ji, Suting Wang, Ping Xiong, Li Zhang, Qing Xu, Aiqiang Xu, Ning Cui
Summary: This study collected sewage samples from Jinan, China from 2018 to 2019, and analyzed classic and novel HAstVs using real-time quantitative PCR and next generation sequencing. The results showed that sewage contains a high diversity of astroviruses, and there are multiple co-circulating lineages within each local HAstV genotype.
Article
Public, Environmental & Occupational Health
Katelin V. Gali, Rachael M. M. St Jacques, Cheyanne I. D. Daniels, Allison O'Rourke, Lauren Turner
Summary: The genomic data generated from next-generation sequencing (NGS) provides nucleotide-level resolution of bacterial genomes which is critical for disease surveillance and the implementation of prevention strategies to interrupt the spread of antimicrobial resistance (AMR) bacteria. Through phylogenetic tools that assess the single nucleotide polymorphisms (SNPs) within a pathogen genome dataset, public health scientists can estimate the genetic identity between isolates. This information is used as an epidemiologic proxy of a putative outbreak.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Entomology
James Devillers, Jean-Philippe David, Benoit Barres, Haoues Alout, Bruno Lapied, Sebastien Chouin, Isabelle Dusfour, Catherine Billault, Farida Mekki, Isabelle Attig, Vincent Corbel
Summary: The present work proposes an integrated plan of resistance surveillance in France and in the French Overseas territories to provide appropriate responses to the situation. The plan includes periodic monitoring of insecticide resistance at the population level using various approaches and a stratification of resistance risk at the territory level to adjust surveillance and vector control actions.
Article
Veterinary Sciences
Adriana Higuera, Giovanny Herrera, Paula Jimenez, Diego Garcia-Corredor, Martin Pulido-Medellin, Diana M. Bulla-Castaneda, Juan Carlos Pinilla, Darwin A. Moreno-Perez, Jenny G. Maloney, Monica Santin, Juan David Ramirez
Summary: This study in Colombia molecularly characterized Blastocystis in fecal samples of domestic animals, identifying eleven subtypes including a novel subtype, ST32. The findings highlight the presence of Blastocystis subtypes with zoonotic potential in farm animals, suggesting a potential role in transmission to humans.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Biochemical Research Methods
Sergey Knyazev, Lauren Hughes, Pavel Skums, Alexander Zelikovsky
Summary: Advancements in next-generation sequencing have enabled detailed assessment of viral population complexity within hosts, extracting crucial epidemiological and biomedical information. However, the complex analysis of NGS data is required to handle the rapidly mutating viral populations.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Pharmacology & Pharmacy
Daniele Carvalho Abrantes, Carolina Barbara Rogerio, Jhones L. de Oliveira, Estefania V. R. Campos, Daniele Ribeiro de Araujo, Laurindo Cesar Pampana, Marcelo Jose Duarte, Georgio Freesz Valadares, Leonardo Fernandes Fraceto
Summary: Arboviral diseases pose a threat to global public health, with a significant percentage of the world population at risk. Mosquito repellents are an effective strategy but may not be accessible to lower-income populations. The development of nanostructured lipid carriers loaded with repellents shows promise in providing effective, stable, and safe protection against arthropod-borne viruses.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
Muddassar Hameed, Sawar Khan, Jinpeng Xu, Junjie Zhang, Xin Wang, Di Di, Zheng Chen, Muhammad Naveed Anwar, Abdul Wahaab, Xiaochun Ma, Mohsin Nawaz, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Jianchao Wei, Zhiyong Ma
Summary: Mosquito samples collected from animal farms near highly populated cities in Xinjiang showed detection of Japanese encephalitis virus, with Bayesian analysis suggesting a likely origin from China, emphasizing the need for further surveillance.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Nnomzie C. Atama, Irina Chestakova, Erwin de Bruin, Tijs J. van den Berg, Emmanuelle Munger, Chantal Reusken, Bas B. Oude Munnink, Henk van der Jeugd, Judith M. A. van den Brand, Marion P. G. Koopmans, Reina S. Sikkema
Summary: Wild birds, especially feathers, are suitable sample types for monitoring arboviruses in bird surveillance. Involvement of citizens in collecting samples from wild birds is feasible. However, optimization is needed for DBS-based antibody detections.
Review
Cell Biology
Tamer Butto, Kanak Mungikar, Peter Baumann, Jennifer Winter, Beat Lutz, Susanne Gerber
Summary: In the last decade, there has been a significant increase in nuclei-based studies, especially in combination with next-generation sequencing. These studies aim to understand the molecular states in heterogeneous cell populations using more affordable sequencing approaches and optimized nuclei isolation methods. While these powerful new methods offer unprecedented insights, it is important to consider the associated challenges critically. This article provides a comprehensive overview of the rise of nuclei-based studies and discusses their advantages and disadvantages, with a specific focus on their utility for transcriptomic sequencing analyses. Improving experimental designs and using various strategies appropriately will lead to biologically accurate and meaningful information.
Article
Parasitology
Andre B. B. Wilke, Chalmers Vasquez, Augusto Carvajal, Maday Moreno, William D. Petrie, John C. Beier
Summary: This study compared the relative abundance, species richness, and community composition of mosquitoes collected in natural and rural areas using BG-Sentinel and CDC light traps. The results showed that both traps had similar sampling power, indicating their suitability for mosquito surveillance in rural and natural areas.
PARASITES & VECTORS
(2022)
Article
Microbiology
Doniddemberel Altantogtokh, Abigail A. Lilak, Ratree Takhampunya, Jira Sakolvaree, Nitima Chanarat, Graham Matulis, Betty Katherine Poole-Smith, Bazartseren Boldbaatar, Silas Davidson, Jeffrey Hertz, Buyandelger Bolorchimeg, Nyamdorj Tsogbadrakh, Jodi M. Fiorenzano, Erica J. Lindroth, Michael E. von Fricken
Summary: Tick-borne diseases are a major public health concern in Mongolia, especially for nomadic pastoralists. This study identified several tick-borne pathogens in Dermacentor ticks, with Rickettsia having the highest infection rate. Livestock play a significant role in disease maintenance. The detection of Anaplasma, Bartonella, and Rickettsia highlights the increased risk for infection throughout Mongolia. Further research is needed to characterize tick-borne pathogens in other endemic tick species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Bassel Nazha, Tony Z. Zhuang, Hiba Dada, Leylah M. Drusbosky, Jacqueline T. Brown, Deepak Ravindranathan, Bradley C. Carthon, Omer Kucuk, Jamie Goldman, Viraj A. Master, Mehmet Asim Bilen
Summary: This study evaluated the genomic landscape of adrenocortical carcinoma (ACC) using blood-based DNA testing. The findings suggest that a significant proportion of ACC patients have actionable mutations, which could inform personalized treatment options or clinical trials for this aggressive malignancy.
Article
Virology
Manasi Majumdar, Cristina Celma, Elaine Pegg, Krunal Polra, Jake Dunning, Javier Martin
Summary: The study focused on a detection method for enteroviruses using NGS analysis of clinical samples, showing high concordance with conventional Sanger sequencing and good sensitivity and specificity for different EV types. The NGS method identified previously untyped EVs and potential co-infections, demonstrating its potential for comprehensive EV detection and genetic analysis.
Review
Clinical Neurology
Christian Mawrin, Ralf Koch, Natalie Waldt, I. Erol Sandalcioglu, Werner E. K. Braunsdorf, Jan-Peter Warnke, Felix Goehre, Hans-Juergen Meisel, Christian Ewald, Sina Neyazii, Ulrich Schueller, Elmar Kirches
Summary: This study utilizes a custom-made next generation sequencing meningioma panel to detect mutations in 11 different genes in a cohort of 109 meningioma patients. The most frequent mutations were found in NF2, AKT1(E17K), and TRAF7. Combinations of different gene mutations were common, and different mutations were associated with different meningioma subtypes and pathological grades.
Article
Biochemistry & Molecular Biology
Hye Lim Choi, Ha Rim Yang, Ha Gyeong Shin, Kyusang Hwang, Ji Woong Kim, Ji Hyun Lee, Taehoon Ryu, Yushin Jung, Sukmook Lee
Summary: Antibody phage display is a crucial technology for discovering and developing target-specific monoclonal antibodies. This study constructed a large human combinatorial single-chain variable fragment library with high diversity, which can be used for the rapid development of recombinant human monoclonal antibodies for therapeutic and diagnostic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Daniel R. Timmins, Kyran M. Staunton, Dagmar B. Meyer, Michael Townsend, Christopher J. Paton, Ana L. Ramirez, Scott A. Ritchie
JOURNAL OF MEDICAL ENTOMOLOGY
(2018)
Article
Infectious Diseases
Agathe M. G. Colmant, Sonja Hall-Mendelin, Scott A. Ritchie, Helle Bielefeldt-Ohmann, Jessica J. Harrison, Natalee D. Newton, Caitlin A. O'Brien, Chris Cazier, Cheryl A. Johansen, Jody Hobson-Peters, Roy A. Hall, Andrew F. van den Hurk
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Entomology
Ana L. Ramirez, Sonja Hall-Mendelin, Glen R. Hewitson, Jamie L. McMahon, Kyran M. Staunton, Scott A. Ritchie, Andrew F. van den Hurk
JOURNAL OF MEDICAL ENTOMOLOGY
(2019)
Article
Entomology
Dagmar B. Meyer, Ana L. Ramirez, Andrew F. van den Hurk, Nina Kurucz, Scott A. Ritchie
JOURNAL OF MEDICAL ENTOMOLOGY
(2019)
Article
Parasitology
Ana L. Ramirez, Andrew F. van den Hurk, Ian M. Mackay, Annie S. P. Yang, Glen R. Hewitson, Jamie L. McMahon, Justin A. Boddey, Scott A. Ritchie, Sara M. Erickson
PARASITES & VECTORS
(2019)
Article
Virology
Danilo Lemos, Jackson B. Stuart, William Louie, Anil Singapuri, Ana L. Ramirez, Jennifer Watanabe, Jodie Usachenko, Rebekah Keesler, Claudia Sanchez-San Martin, Tony Li, Calla Martyn, Glenn Oliveira, Sharada Saraf, Nathan D. Grubaugh, Kristian G. Andersen, James Thissen, Jonathan Allen, Monica Borucki, Konstantin A. Tsetsarkin, Alexander G. Pletnev, Charles Y. Chiu, Koen K. A. Van Rompay, Lark L. Coffey
JOURNAL OF VIROLOGY
(2020)
Article
Virology
Natalee D. Newton, Agathe M. G. Colmant, Caitlin A. O'Brien, Emma Ledger, Devina Paramitha, Helle Bielefeldt-Ohmann, Daniel Watterson, Breeanna J. McLean, Sonja Hall-Mendelin, David Warrilow, Andrew F. van den Hurk, Wenjun Liu, Christina Hoare, Joanne R. Kizu, Penelope J. Gauci, John Haniotis, Stephen L. Doggett, Babak Shaban, Cheryl A. Johansen, Roy A. Hall, Jody Hobson-Peters
Article
Microbiology
Ana L. Ramirez, Agathe M. G. Colmant, David Warrilow, Bixing Huang, Alyssa T. Pyke, Jamie L. McMahon, Dagmar B. Meyer, Rikki M. A. Graham, Amy Jennison, Scott A. Ritchie, Andrew F. van den Hurk
Article
Entomology
Sonja Hall-Mendelin, Alyssa T. Pyke, Ana L. Ramirez, Kyran M. Staunton, Peter Burtonclay, Jamie McMahon, Jean Barcelon, Andrew F. van den Hurk
Summary: This study examined the impact of mosquito population origin, virus strain, and virus titer on the infection and dissemination of dengue virus type 2 in Aedes aegypti populations from north Queensland. The results suggest that these factors play a significant role in the transmission dynamics of DENV in northern Australia, with implications for the fitness of the sylvatic virus strain in urban Ae. aegypti populations.
JOURNAL OF MEDICAL ENTOMOLOGY
(2021)
Article
Virology
Cassie C. Jansen, Jonathan M. Darbro, Frances A. Birrell, Martin A. Shivas, Andrew F. van den Hurk
Summary: COVID-19 mitigation measures in Queensland, Australia, led to more mosquito-borne diseases being acquired near residential areas due to limited human movement. A significant decrease in international passenger flights resulted in a reduction of imported dengue and malaria cases.
Article
Virology
Alyssa T. Pyke, Neelima Nair, Andrew F. van den Hurk, Peter Burtonclay, Son Nguyen, Jean Barcelon, Carol Kistler, Sanmarie Schlebusch, Jamie McMahon, Frederick Moore
Summary: The emergence of SARS-CoV-2 variants poses challenges to public health disease management, and the replication and evolutionary dynamics of the virus have important implications for the development of vaccines, antiviral therapies, and epidemiological control strategies for COVID-19.
Review
Virology
Andrew F. van den Hurk, Eloise Skinner, Scott A. Ritchie, John S. Mackenzie
Summary: In early 2022, Japanese encephalitis virus (JEV) was found to be the cause of stillborn and mummified piglets in pig farms in southeastern Australia. Human cases and infected piglets in other pig farms were subsequently identified across a wide area. The primary vectors of JEV in Australia should be considered as members of the Culex sitiens subgroup, particularly Culex annulirostris. Information on other mosquito species and additional factors that could impact JEV transmission are also discussed.
Review
Infectious Diseases
Andrew F. van den Hurk
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2018)
Article
Microbiology
John S. Mackenzie, Andrew F. van den Hurk
MICROBIOLOGY AUSTRALIA
(2018)