Article
Chemistry, Multidisciplinary
Yiyin Xia, Janet To, Ning-Yu Chan, Side Hu, Heng Tai Liew, Seetharamsing Balamkundu, Xiaohong Zhang, Julien Lescar, Surajit Bhattacharjya, James P. Tam, Chuan-Fa Liu
Summary: This study found that Asn(OH) is an unnatural substrate for PALs, and successfully synthesized a new MMP2 inhibitor cyclic peptide using Asn(OH)-mediated cyclization, with the hydroxamic acid moiety as the key pharmacophore. Several bioactive Asp-containing cyclic peptides were also synthesized using this approach, demonstrating the effectiveness of substrate engineering in expanding the application of PAL ligation in the synthesis of therapeutic cyclic peptides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Fisheries
Marjana dos Santos Cardoso, Antonio Cesar Godoy, Jarred Hugh Oxford, Romulo Rodrigues, Matheus dos Santos Cardoso, Fabio Bittencourt, Altevir Signor, Wilson Rogerio Boscolo, Aldi Feiden
Summary: The study evaluated the nutritional value and digestibility of protein hydrolysates from poultry and swine meat by-products for juvenile Nile tilapia. Significant differences were found between the various protein hydrolysates in almost all nutritional fractions analyzed, with high apparent digestibility rates observed.
Article
Biochemistry & Molecular Biology
Kaley M. Mientkiewicz, Leila Peraro, Joshua A. Kritzer
Summary: This study adapted the chloroalkane penetration assay to screen peptide libraries in a high-throughput, quantitative, and automation-ready manner, effectively exploring how the number, patterning, and stereochemistry of arginine residues affect the cytosolic penetration of a model 10-residue peptide.
ACS CHEMICAL BIOLOGY
(2021)
Article
Plant Sciences
Wenjuan Ding, Danmei Tian, Mei Chen, Zixuan Xia, Xiyang Tang, Sihao Zhang, Jihua Wei, Xunuo Li, Xinsheng Yao, Bin Wu, Jinshan Tang
Summary: LC-MS/MS-based molecular networking facilitated the discovery of seven new cyclopentapeptides, pseudoviridinutans A-F (1-7), from the marine-derived fungus Aspergillus pseudoviridinutans TW58-5. These compounds displayed a rare amino acid moiety, O,& beta;-dimethyltyrosine, which was observed for the first time from a marine-derived fungus. The planar structures of compounds 1-7 were elucidated through various spectroscopic analyses, and their absolute configurations were determined using Marfey's method and X-ray diffraction. Compound 6 exhibited significant anti-inflammatory activity by inhibiting the production of nitric oxide in LPS-induced murine macrophage RAW264.7 cells.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Chemistry, Analytical
Takayuki Kawai, Yasuhiro Mihara, Makiko Morita, Masahiko Ohkubo, Taiji Asami, Tomonobu M. Watanabe
Summary: Cyclic peptides (CPs) are considered next-generation drugs due to their cell-permeable potential and specific affinity, and single-cell cytoplasm mass spectrometry (SCC-MS) is a promising method for assessing their intracellular retention and transmembrane permeability.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Takayuki Katoh, Hiroaki Suga
Summary: Genetic code manipulation allows the synthesis of peptide libraries with diverse nonproteinogenic amino acids, facilitating the discovery of bioactive peptides. Recent advancements in using engineered tRNAs have significantly improved the efficiency of incorporating D-, ss-, and gamma-amino acids into nascent peptides.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2021)
Review
Pharmacology & Pharmacy
Maria Gallo, Sira Defaus, David Andreu
Summary: G protein-coupled receptors (GPCRs) are usually described as monomeric transmembrane receptors, but increasing evidence shows that many GPCRs actually form higher-order assemblies. The formation, structure, physiological roles, and potential therapeutic applications of these assemblies are actively being explored. This review provides a comprehensive overview of GPCR complexes, with a focus on disrupting GPCRs using peptides mimicking transmembrane domains involved in multimerization. Recent strategies for developing drug-like versions of these transmembrane peptide candidates for therapeutic applications are also highlighted.
Article
Chemistry, Multidisciplinary
Takahito Ito, Hidetomo Yokoo, Takuma Kato, Mitsunobu Doi, Yosuke Demizu
Summary: In this study, a new cyclic peptide with a different amino acid arrangement was designed, leading to a change in the molecular conformation. The study demonstrates the potential to obtain diverse conformations by manipulating the arrangement of amino acids, providing a direction for developing new applications.
Article
Chemistry, Multidisciplinary
Vincent Faugeras, Olivier Duclos, Didier Bazile, Abdou Rachid Thiam
Summary: This study focuses on the passive permeation of hydrophobic cyclic peptides inspired by somatostatin and investigates the impact of peptide cyclization and backbone methylation on translocation rates. The results show that cyclization improves peptide permeability, while methylation does not. Additionally, the study identifies membrane insertion and peptide intrinsic diffusion as two independent factors affecting permeability. Compared to other models, droplet interface bilayers (DIBs) provide intermediate membrane permeability values.
Article
Chemistry, Analytical
Qingfang Niu, Pengyue Jin, Yu Huang, Lifang Fan, Caihong Zhang, Cheng Yang, Chuan Dong, Wenting Liang, Shaomin Shuang
Summary: In this study, a novel nanocomposite 3D-rGO/Pd@Au/CM-beta-CD was developed for the electrochemical enantiorecognition of tyrosine. Under the optimized determining conditions, this approach accurately determines the relative content of l- or d-Tyr enantiomers in a racemic solution.
Article
Chemistry, Multidisciplinary
George J. Saunders, Andrei K. Yudin
Summary: The passive membrane permeability of macrocycles can be greatly improved by incorporating heterocyclic grafts. However, the permeability does not scale linearly with the number of heterocycles, highlighting the subtleties of the conformation-property relationship in this class of molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Zhidong Chen, Yongxiao Li, Xinpei Wang, Xiaohui Qiu, Chenglin Wang, Zhe Wang, Xu Chen, Junqing Wang
Summary: In this study, a high-throughput molecular dynamics screening (HTMDS) process was proposed for the design of stable cyclic peptide (CP) binders using a combinatorial CP library composed of canonical and non-canonical amino acids. The methods were applied to design CP inhibitors for the bromodomain (BrD) of ATAD2B, and the results showed that CP-1(st).43 was the best CP candidate with an estimated Delta Gbind of -28.48 kcal/mol compared to the standard inhibitor C-38 which exhibited Delta Gbind of -17.11 kcal/mol.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Organic
Kiran Bajaj, Karishma Pidiyara, Shahid Khan, Prabhat N. Jha, Rajeev Sakhuja, Dalip Kumar
Summary: This study focused on the synthesis and characterization of fluorescent labeled glutamine and asparagine derivatives with high fluorescence quantum yields and excellent photostabilities using traceless Staudinger ligation. The successful permeation of these fluorescent amino acids into cellular components demonstrated their potential as fluorescent probes for chemical biology.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Joongoo Lee, Kevin J. Schwarz, Hao Yu, Antje Kruger, Eric Anslyn, Andrew D. Ellington, Jeffrey S. Moore, Michael C. Jewett
Summary: This study reports a method for fluorescent labeling of amino acids catalyzed by flexizyme, and demonstrates the site-specific incorporation of these fluorescent amino acids into peptides by the ribosome in vitro through genetic code reprogramming.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Ignacio Perez-Victoria, Gloria Crespo, Fernando Reyes
Summary: The determination of amino acid chirality in natural peptides can be achieved through Marfey's analysis. However, for certain amino acids with a second chiral center, distinguishing between the two possible epimers can be challenging. This study presents a new approach using HPLC-SPE-NMR to overcome this challenge.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Adrian L. Pietkiewicz, Yuqi Zhang, Marwa N. Rahimi, Michael Stramandinoli, Matthew Teusner, Shelli R. McAlpine
ACS MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Chemistry, Medicinal
Samantha S. Zaiter, Yuantao Huo, Fong Y. Tiew, Jason E. Gestwicki, Shelli R. McAlpine
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Yuantao Huo, Laura K. Buckton, Jack L. Bennett, Eloise C. Smith, Frances L. Byrne, Kyle L. Hoehn, Marwa N. Rahimi, Shelli R. McAlpine
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Fatemeh Mirnajafizadeh, Deborah Ramsey, Shelli McAlpine, Fan Wang, John Arron Stride
Review
Chemistry, Multidisciplinary
Laura K. Buckton, Marw N. Rahimi, Shelli R. McAlpine
Summary: Developing macrocyclic peptides that can reach intracellular targets is a significant challenge, and current chemical strategies can improve cell permeability, but often at the expense of biological activity.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Medicinal
Jessica Kho, P. Chi Pham, Suhyeon Kwon, Alana Y. Huang, Joel P. Rivers, Huixin Wang, Heath Ecroyd, W. Alexander Donald, Shelli R. McAlpine
Summary: This study presents the development of small molecule peptides derived from the N-terminal sequence of Hsp27 protein, demonstrating the ability to directly regulate protein aggregation, offering a new approach for drug development in controlling protein aggregation.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Zifei Han, Shelli R. McAlpine, Robert Chapman
Summary: This study demonstrates the successful delivery of inhibitors to block the interaction between Hsp70 and HOP using polymer nanogels. The results show the effectiveness of these inhibitors in inhibiting Hsp70 and inducing cancer cell death.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jeanette R. McConnell, H. Jane Dyson, Shelli R. McAlpine
Summary: This study reveals the interaction between Hsp90 protein and various inhibitors and modulators, showing their binding sites within different functional domains of the protein.
RSC MEDICINAL CHEMISTRY
(2021)
Article
Polymer Science
Shegufta Farazi, Fan Chen, Henry Foster, Raelene Boquiren, Shelli R. McAlpine, Robert Chapman
Article
Chemistry, Multidisciplinary
Marwa N. Rahimi, Henry G. Foster, Shegufta N. Farazi, Robert Chapman, Shelli R. McAlpine
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Marwa N. Rahimi, Shelli R. McAlpine
CHEMICAL COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Leo L. H. Lee, Laura K. Buckton, Shelli R. McAlpine
Article
Chemistry, Multidisciplinary
Yao Wang, Yen Chin Koay, Shelli R. McAlpine
CHEMISTRY-A EUROPEAN JOURNAL
(2017)
Article
Chemistry, Medicinal
Yao Wang, Yen Chin Koay, Shelli R. McAlpine