Journal
OPTICS LETTERS
Volume 43, Issue 13, Pages 3152-3155Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.43.003152
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Funding
- Russian Science Foundation (RSF) [14-25-00129]
- Russian Science Foundation [17-25-00015] Funding Source: Russian Science Foundation
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While laser scanning fluorescence lifetime imaging (FLIM) is a powerful approach for cell biology, its small field of view (typically less than 1 mm) makes it impractical for the imaging of large biological samples that is often required for biomedical applications. Here we present a system that allows performing FLIM on macroscopic samples as large as 18 mm with a lateral resolution of 15 mu m. The performance of the system is verified with FLIM of endogenous metabolic cofactor reduced nicotinamide adenine dinucleotide (phosphate), NAD(P)H, and genetically encoded fluorescent protein mKate2 in a mouse tumor in vivo. (C) 2018 Optical Society of America
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