Article
Endocrinology & Metabolism
Hui Li, E. Cai, Hongyan Cheng, Xue Ye, Ruiqiong Ma, Honglan Zhu, Xiaohong Chang
Summary: This study demonstrates that FGA can enhance the interaction between endometrial stromal cells and HUVECs through the potential VEGA-VEGFR-FAK signaling axis, promoting angiogenesis in EM.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuechao Dong, Guixing Ma, Xiaoting Hou, Yingying Han, Zhen Ding, Wanze Tang, Litong Chen, Yangshan Chen, Bo Zhou, Feng Rao, Kaosheng Lv, Changzheng Du, Huiling Cao
Summary: Kindlin-2 plays critical roles in the development and homeostasis of multiple key organs, and our study reveals its importance in NOTCH-mediated physiological angiogenesis. We demonstrate that KINDLIN-2 enhances various functions of endothelial cells induced by VEGF-A and is crucial for maintaining the integrity of NOTCH1. The deficiency of KINDLIN-2 leads to impaired angiogenesis and avascular retinas, which can be rescued by the inhibitor DAPT, and we also find that KINDLIN-2 is involved in hyperactive angiogenesis stimulated by high glucose and could be a potential therapeutic target for diabetic retinopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Integrative & Complementary Medicine
Yulin He, Hiotong Kam, Xue Wu, Qian Chen, Simon Ming Yuen Lee
Summary: This study aimed to further investigate the anti-osteoporosis effect of aucubin in transgenic medaka and its pro-angiogenic effect and mechanism of action in vivo and in vitro. The results showed that aucubin can reduce bone resorption, improve vascular insufficiency, and promote proliferation, migration, and tube formation of endothelial cells by regulating signaling pathways.
Review
Biochemistry & Molecular Biology
Zay Yar Soe, Eun Jeong Park, Motomu Shimaoka
Summary: Integrins play a crucial role in cell adhesion, intercellular communication, and functions of tumor and immune cell exosomes, with potential implications in tumor microenvironment modulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Carlos Mateluna, Pedro Torres, Marcelo Rodriguez-Pena, Patricio Silva, Douglas J. Matthies, Alfredo Criollo, Floris J. Bikker, Jan G. M. Bolscher, Christian A. M. Wilson, Gerald Zapata-Torres, Vicente A. Torres
Summary: In this study, it was found that VEGFR2 is the endothelial cell receptor of Histatin-1, revealing the mechanism by which Histatin-1 promotes endothelial cell migration and angiogenesis.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Oncology
YanJie Lu, HanZheng Zhao, Ying Liu, YanZhen Zuo, Qian Xu, Lei Liu, XiaoMin Li, HongBin Zhu, Ying Zhang, Shuling Zhang, XiangYang Zhao, YuHong Li
Summary: Chronic stress can exacerbate tumor angiogenesis, primarily through elevating stress-related hormone levels and causing behavioral changes in individuals. Utilizing isoprenaline as an agonist of beta 2-adrenergic receptor, the study demonstrated the molecular mechanisms of stress in tumor angiogenesis both in vivo and in vitro. Isoprenaline promotes VEGF autocrine in HUVECs, up-regulating plexinA1 and VEGFR2 levels, leading to activation of the JAK2-STAT3 signaling pathway essential for angiogenesis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
William R. Critchley, Gina A. Smith, Ian C. Zachary, Michael A. Harrison, Sreenivasan Ponnambalam
Summary: VEGFR2, encoded by KDR, plays a crucial role in endothelial function and angiogenesis. UBE2D1 and UBE2D2 are identified as key regulators of VEGFR2 ubiquitination and proteolysis, affecting VEGF-A signaling and endothelial tubulogenesis.
JOURNAL OF CELL SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Meng Zhang, Lijun Ding, Zhiqiang Zhou, Chang Liu, Cunkai Wang, Binlin Chen, Xin Chen, Yumei Zhang
Summary: VEGFR2/mTOR/S6K1 signaling plays a regulatory role in Roxarsone-mediated promotion of angiogenesis and enhanced tumor growth.
Review
Cell Biology
Veronica Morandi, Jim Petrik, Jack Lawler
Summary: Thrombospondins (TSPs) are a family of extracellular matrix proteins that regulate cellular behavior and influence biological processes such as angiogenesis and cancer progression. Thrombospondin 1 (TSP-1) interacts with cell surface receptors to have varying effects on cellular behavior depending on the context. Specific membrane microdomains containing receptors like VEGFR2 and Src family kinases are modulated by TSP-1, impacting endothelial cell signal transduction and angiogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Peripheral Vascular Disease
Sheela Nagarkoti, Young-Mee Kim, Dipankar Ash, Archita Das, Eric Vitriol, Tracy-Ann Read, Seock-Won Youn, Varadarajan Sudhahar, Malgorzata McMenamin, Yali Hou, Harriet Boatwright, Ruth Caldwell, David W. Essex, Jaehyung Cho, Tohru Fukai, Masuko Ushio-Fukai
Summary: PDIA1 senses the H2O2 signal induced by VEGF to promote VEGFR2 signaling and angiogenesis in endothelial cells, thereby enhancing postnatal angiogenesis.
Article
Biotechnology & Applied Microbiology
Brianna Guarino, Venkatesh Katari, Ravi Adapala, Neha Bhavnani, Julie Dougherty, Mahmood Khan, Sailaja Paruchuri, Charles Thodeti
Summary: This study demonstrates that t-EVs induce endothelial cell phenotypic transition and abnormal angiogenesis through post-translational downregulation of TRPV4 and activation of the Rho/Rho kinase/YAP/VEGFR2 pathways. Furthermore, t-EVs can induce abnormal vessel formation in vivo independently of tumors, suggesting their potential as novel targets for vascular normalization and cancer therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Yuqing Zhang, Huocong Huang, Morgan Coleman, Arturas Ziemys, Purva Gopal, Syed M. Kazmi, Rolf A. Brekken
Summary: Studies have shown that VEGF induces an immunosuppressive phenotype in VEGFR2* myeloid cells, while VEGF blockade inhibits this phenotype and enhances T cell activation, improving the efficacy of immune checkpoint blockade. This indicates the potential of VEGF inhibition in potentiating immune therapy for cancer.
Article
Peripheral Vascular Disease
Camilla Ariano, Chiara Riganti, Davide Cora, Donatella Valdembri, Giulia Mana, Elena Astanina, Guido Serini, Federico Bussolino, Gabriella Doronzo
Summary: This article investigates the impact of oncogenic transcription factor EB (TFEB) on the adhesion of endothelial cells to the surrounding extracellular matrix (ECM). The study reveals that TFEB promotes turnover of ECM adhesion by regulating cholesterol metabolism, suggesting it may serve as a novel target for therapeutic control of pathological angiogenesis.
Article
Biochemistry & Molecular Biology
Jun-Ping Shiau, Cheng-Che Wu, Shu-Jyuan Chang, Mei-Ren Pan, Wangta Liu, Fu Ou-Yang, Fang-Ming Chen, Ming-Feng Hou, Shen-Liang Shih, Chi-Wen Luo
Summary: The study found a positive correlation between FAK and VEGFR2 in TNBC patients, and FAK promotes angiogenesis in TNBC cells by regulating VEGFR2 expression. Therefore, targeting FAK could be a potential strategy for TNBC treatment.
Article
Plant Sciences
Yan Wang, Hong Wu, Bin-Jie Gui, Jian Liu, Gen-Xiang Rong, Ran Deng, Yan-Hong Bu, Heng Zhang
Summary: The study found that geniposide can inhibit angiogenesis in rheumatoid arthritis and alleviate disease progression. The research also revealed the important role of SphK1, VEGF/VEGFR2, and S1P/S1PR1 signaling pathways in the effect of geniposide on synovial angiogenesis.
Review
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Yuki Ohkawa, Kento Maeda, Yoichiro Harada, Masamichi Nagae, Yasuhiko Kizuka, Hideyuki Ihara, Yoshitaka Ikeda
Summary: Cancer-related changes in glycans on cell surface proteins are regulated by glycosyltransferases and related genes, which play key roles in cancer metastasis and invasion. The significance of EMT and N-glycan branching glycosyltransferases needs to be reconsidered for potential therapeutic strategies. Specific glycosyltransferases involved in EMT or MET pathways may be difficult to define due to their roles in intermediate states.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Yuki Ohkawa, Yoichiro Harada, Naoyuki Taniguchi
Summary: Glycosylation is a common posttranslational modification of proteins, regulated by various glycosyltransferases and glycosidases. The core fucose structure on N-glycans may have protective functions against emphysema and COPD. Glycomimetics using glycan analogs could be a promising therapeutic strategy for various medical conditions.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Review
Biochemistry & Molecular Biology
Yoichiro Harada, Yuki Ohkawa, Kento Maeda, Naoyuki Taniguchi
Summary: The endoplasmic reticulum (ER) contains multiple quality control systems (QCS) that shape the glycoproteome of eukaryotic cells. One of these systems, glycan QCS, plays a crucial role in ensuring proper glycoprotein folding while also potentially increasing the risk of overall hypoglycosylation, leading to protein misfolding and cellular stress response activation.
Editorial Material
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Kento Maeda, Masato Kitano
Summary: This commentary discusses a highly cited paper by Yasuhisa Kono which focused on the mechanism of hydroxylamine autooxidation in the assay of superoxide dismutase activity. Kono's work contributed to the development and progress in enzyme assay systems.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Review
Biochemistry & Molecular Biology
Motoko Takahashi, Yoshihiro Hasegawa, Kento Maeda, Masato Kitano, Naoyuki Taniguchi
Summary: Glycosylation of cell surface receptors plays a crucial role in regulating signal transduction. This review focuses on the role of glycosyltransferases involved in N-glycan modification, specifically the effects of branching and elongation on signaling by cell surface receptors. The review also discusses the involvement of these enzymes in EMT/MET programs related to differentiation, cancer development, progression, and therapy resistance.
GLYCOCONJUGATE JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Tetsuya Hirata, Atsushi Kobayashi, Tamio Furuse, Ikuko Yamada, Masaru Tamura, Hiroyuki Tomita, Yuko Tokoro, Akinori Ninomiya, Yoshitaka Fujihara, Masahito Ikawa, Yusuke Maeda, Yoshiko Murakami, Yasuhiko Kizuka, Taroh Kinoshita
Summary: The GPI-GalNAc side chain is essential for the functions of GPI-APs in mammals, particularly in bone and the brain. Knocking out the initiation enzyme PGAP4 in mice leads to various phenotypes and increased vulnerability to prion diseases, highlighting the protective roles of the GalNAc side chain.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Reina F. Osuka, Tetsuya Hirata, Masamichi Nagae, Miyako Nakano, Hiroyuki Shibata, Ryo Okamoto, Yasuhiko Kizuka
Summary: The N-terminal domain of N-acetylglucosaminyltransferase-V (GnT-V) is crucial for efficient glycosylation and plays a key role in the recognition of glycoprotein substrates. This study revealed that the N domain interacts directly with the substrate polypeptide, providing new insights into the mechanism of substrate-selective biosynthesis of N-glycans.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Biographical-Item
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Ernesto Carafoli
Summary: This article expresses gratitude and admiration for Dr. William Lennarz for his contributions and accomplishments in the field of life science during his tenure as Editor-in-Chief of Biochemical and Biophysical Research Communications.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Kiyotaka Kondo, Yoichiro Harada, Miyako Nakano, Takehiro Suzuki, Tomoko Fukushige, Ken Hanzawa, Hirokazu Yagi, Koichi Takagi, Keiko Mizuno, Yasuhide Miyamoto, Naoyuki Taniguchi, Koichi Kato, Takuro Kanekura, Naoshi Dohmae, Kentaro Machida, Ikuro Maruyama, Hiromasa Inoue
Summary: Protein N-glycosylation in lung cancer sEVs may potentially reflect the histology of lung cancers.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuriko Tachida, Saori Miura, Yui Muto, Hiroyuki Takuwa, Naruhiko Sahara, Akihiro Shindo, Yukio Matsuba, Takashi Saito, Naoyuki Taniguchi, Yasushi Kawaguchi, Hidekazu Tomimoto, Takaomi Saido, Shinobu Kitazume
Summary: The deposition of amyloid P in blood vessels of the brain is commonly observed in Alzheimer's disease patients. However, it is difficult to examine the contribution of cerebral amyloid angiopathy (CAA) to the pathogenesis of Alzheimer's disease in mouse models. Based on biochemical analyses, the researchers hypothesized that endothelial APP expression would be lower in rodents and subsequently generated mice that express human WT APP in endothelial cells. The mice showed increased levels of serum A beta and soluble APP, indicating the crucial role of endothelial APP in blood A beta levels. Crossing these mice with a specific APP knock-in mouse model led to an expanded CAA pathology.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Dermatology
Kota Tachibana, Yuki Ohkawa, Noriko Kanto, Kento Maeda, Shuichi Ohe, Taiki Isei, Yoichiro Harada, Naoyuki Taniguchi
Summary: Glycan expression plays an important role in melanoma cells, and the ectopic expression of keratan sulfate (KS) can enhance the invasiveness and adhesion capabilities of melanoma cells, as well as stimulate the phosphorylation of paxillin, contributing to the development of malignant phenotypes.
JOURNAL OF DERMATOLOGY
(2022)
Article
Biology
Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
Summary: The activity of GnT-V in cells is regulated by changing the maturity of cellular N-glycans. Altering the glycosylation state affects the secretion and protein level of GnT-V, which in turn impacts its promotion of cancer growth and malignancy.
COMMUNICATIONS BIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Naoyuki Taniguchi
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Reina F. Osuka, Masamichi Nagae, Akito Ohuchi, Shiho Ohno, Yoshiki Yamaguchi, Yasuhiko Kizuka
Summary: In this study, it was found through molecular dynamics simulations and biochemical experiments that residues outside the catalytic pocket of GnT-V are likely involved in the recognition of the core part of N-glycan. Additionally, the simulation suggested that UDP binding affects the orientation of the acceptor due to conformational changes. These findings provide new insights into how GnT-V recognizes its glycoprotein substrates.
Article
Biochemistry & Molecular Biology
Masato Kitano, Yasuhiko Kizuka, Tomoaki Sobajima, Miyako Nakano, Kazuki Nakajima, Ryo Misaki, Saki Itoyama, Yoichiro Harada, Akihiro Harada, Eiji Miyoshi, Naoyuki Taniguchi
Summary: Glycosylation is a common posttranslational modification of proteins that is tightly regulated by the subcellular location and transportation of glycosyltransferases. Knockdown of Rab11 results in enhanced sialylation of N-glycans, specifically through the altered localization of the alpha 2,3-sialyltransferase ST3GAL4. This indicates a novel mechanism of glycosyltransferase regulation by Rab11 in post-Golgi compartments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)