Journal
NUCLEIC ACIDS RESEARCH
Volume 46, Issue 15, Pages 7631-7642Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky526
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Funding
- NCI [CA190911A]
- IDB RAS government programs of basic research [0108-2018-0001]
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DNA in nucleosomes has restricted nucleosome dynamics and is refractory to DNA-templated processes. Histone post-translational modifications play important roles in regulating DNA accessibility in nucleosomes. Whereas most histone modifications function either by mitigating the electrostatic shielding of histone tails or by recruiting 'reader' proteins, we show that ubiquitylation of H2B K34, which is located in a tight space protected by two coils of DNA superhelix, is able to directly influence the canonical nucleosome conformation via steric hindrances by ubiquitin groups. H2B K34 ubiquitylation significantly enhances nucleosome dynamics and promotes generation of hexasomes both with symmetrically or asymmetrically modified nucleosomes. Our results indicate a direct mechanism by which a histone modification regulates the chromatin structural states.
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