Journal
NEW JOURNAL OF CHEMISTRY
Volume 42, Issue 5, Pages 3503-3508Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nj04791f
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Funding
- National Natural Science Foundation of China [21775132, 21402168, 21505112]
- Scientific Research Foundation of Hunan Provincial Education Department [16A204]
- Hunan Collaborative Innovation Centre of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization
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This study demonstrated fluorescent detection of Japanese encephalitis virus (JEV) by surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on silica microspheres (SiMP) with JEV as the template, (3-aminopropyl)triethoxysilane (APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed with SiMP, and the polymerization was performed in mild condition. After polymerization, templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV-MIPs can selectively recognize JEV in the presence of Hepatitis A virus (HAV), Simian virus 40 (SV40) and Rabies virus (RV), and also sensitively detect JEV with a picomolar detection limit.
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