Article
Cell Biology
Ayaka Sugeno, Wenhui Piao, Miki Yamazaki, Kiyofumi Takahashi, Koji Arikawa, Hiroko Matsunaga, Masahito Hosokawa, Daisuke Tominaga, Yoshio Goshima, Haruko Takeyama, Toshio Ohshima
Summary: Recent studies have shown that mutation at Ser522 inhibits CRMP2 phosphorylation, leading to axon elongation and partial recovery of sensorimotor function after SCI. Transcriptome analysis in CRMP2KI mice revealed suppressed structural pathophysiology in sensorimotor cortex and differential regulation of gene expression related to energy metabolism and neuronal pathways.
NEURAL REGENERATION RESEARCH
(2021)
Article
Cell Biology
Shogo Yoshihara, Xuguang Jiang, Momo Morikawa, Tadayuki Ogawa, Sotaro Ichinose, Hirooki Yabe, Akiyoshi Kakita, Manabu Toyoshima, Yasuto Kunii, Takeo Yoshikawa, Yosuke Tanaka, Nobutaka Hirokawa
Summary: Research has shown that a high-betaine diet can alleviate certain symptoms of schizophrenia, such as neuronal morphogenesis and behaviors, potentially related to downregulation of KIF3 expression levels. This suggests a possible pathway for alleviating human schizophrenia pathogenesis through betaine administration.
Article
Neurosciences
Longfei Cheng, Keen Chen, Jiong Li, Jiaming Wu, Jiaqi Zhang, Li Chen, Guoqing Guo, Jifeng Zhang
Summary: CRMP2 can promote the surface expression of AMPA receptor GluA1 subunit in hippocampal neurons and in HEK293T cells expressing GluA1 subunits, through interaction with GluA1 and regulation by Cdk5 phosphorylation.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Yunfeng Jin, Saiyan Bian, Hui Wang, Jiahang Mo, He Fei, Li Li, Tong Chen, Hua Jiang
Summary: Cancer associated fibroblasts (CAFs) play a crucial role in the progression of ovarian cancer (OvCA). The conditioned medium of primary CAFs can promote proliferation, migration, and invasion of ovarian cancer cells. It was found that the microtubule-associated protein CRMP2, derived from CAFs, is a key regulator responsible for mediating these cell events of OvCA.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Analytical
Yumi Kwon, Seonjeong Lee, Narae Park, Shinyeong Ju, Sungho Shin, Seongjin Yoo, Hankyul Lee, Cheolju Lee
Summary: Phosphorylation is a key component of cellular signaling cascades, controlling important cellular functions. However, phosphoproteomic analysis is typically limited by the amount of sample required for phosphopeptide enrichment. In this study, a new strategy was developed to increase phosphoproteomic signals from a small amount of sample without phosphoenrichment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hisato Suzuki, Simo Li, Tomoharu Tokutomi, Chisen Takeuchi, Miyuki Takahashi, Mamiko Yamada, Hironobu Okuno, Fuyuki Miya, Toshiki Takenouchi, Hironao Numabe, Kenjiro Kosaki, Toshio Ohshima
Summary: In this study, two patients with CRMP2 non-synonymous variants were described, showing intellectual disability and hypoplasia of the corpus callosum. Functional assays using zebrafish and cellular experiments demonstrated that these variants resulted in the loss-of-function of CRMP2 protein, supporting the idea that pathogenic variants in CRMP2 can cause intellectual disabilities in humans.
HUMAN MOLECULAR GENETICS
(2022)
Article
Neurosciences
Yuki Yamazaki, Maho Moizumi, Jun Nagai, Yoshiki Hatashita, Tianhong Cai, Papachan Kolattukudy, Takafumi Inoue, Yoshio Goshima, Toshio Ohshima
Summary: This study demonstrated the importance of Cdk5-mediated phosphorylation of CRMP2 in neuronal migration in the developing cerebral cortex, with redundant functions of CRMP1 and CRMP4. Experimental results showed that these three proteins play crucial roles in proper neuronal migration.
Article
Biochemical Research Methods
Aydanur Senturk, Ayse T. Sahin, Ayse Armutlu, Murat Can Kiremit, Omer Acar, Selcuk Erdem, Sidar Bagbudar, Tarik Esen, Nurhan Ozlu
Summary: In this study, quantitative phosphoproteomics characterization of ccRCC tumor and normal adjacent tissues revealed significant differential regulation of phosphoproteins between tumor and normal tissues. These differentially regulated phosphoproteins are associated with proliferative and migratory behavior of renal tumors. Furthermore, master kinases responsible for phosphorylation of substrates associated with cell proliferation, inflammation, and migration were identified.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Christian H. Gabriel, Marta del Olmo, Amin Zehtabian, Marten Jaeger, Silke Reischl, Hannah van Dijk, Carolin Ulbricht, Asylkhan Rakhymzhan, Thomas Korte, Barbara Koller, Astrid Grudziecki, Bert Maier, Andreas Herrmann, Raluca Niesner, Tomasz Zemojtel, Helge Ewers, Adrian E. Granada, Hanspeter Herzel, Achim Kramer
Summary: The study introduces an efficient strategy for generating knock-in reporter cell lines using the CRISPR technology to investigate the dynamics of circadian clock proteins. The findings reveal highly rhythmic expressions of PER2 and CRY1 proteins in the nucleus of human cells, with notable differences in abundance and transcriptional rhythms between the two proteins.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Maria Camila Rodriguez Gallo, R. Glen Uhrig
Summary: This mini-review summarizes the current knowledge of plant-specific protein kinase families involved in alternative splicing (AS) regulation and compares them to their counterparts in animals. The extensive phosphorylation of splicing-related proteins in plants is highlighted, underscoring the importance of investigating the impact of AS on protein and post-translational modifications.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Tomas Takac, Pavel Krenek, George Komis, Pavol Vadovic, Miroslav Ovecka, Ludmila Ohnoutkova, Tibor Pechan, Petr Kasparek, Tereza Ticha, Jasim Basheer, Mark Arick, Jozef Samaj
Summary: This study demonstrates the significant role of HvMPK3 in barley's response to the bacterial elicitor flg22, with knock-out lines showing downregulation of defense-related proteins and reduced susceptibility to flg22. The differential proteomic analysis and phenotypic observations suggest a specific molecular and root hair response of barley to flg22 in HvMPK3 KO lines.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Endocrinology & Metabolism
Xiangmei Yu, Xiaomei Chen, Weiting Liu, Menghong Jiang, Zhifu Wang, Jing Tao
Summary: The study showed that electro-acupuncture (EA) treatment increased mechanical pain threshold and decreased fasting blood glucose levels in diabetic rats. Proteomics analysis revealed significant changes in proteins involved in oxidative stress injury regulation during EA effects on diabetic painful neuropathy.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Divya Ramachandran, Kusum Sharma, Vishal Saxena, Niepukolie Nipu, Dinusha C. Rajapaksha, Jan A. Mennigen
Summary: The nonapeptide vasotocin/vasopressin is highly conserved and plays a role as neuromodulator and signaling molecule in vertebrates. Pharmacological studies in several teleost fishes suggest that vasotocin has sex and species-specific reproductive roles. However, comprehensive studies investigating the behavioral and physiological consequences of genetic ablation of vasotocin in a genetically tractable fish model, such as the zebrafish, are currently lacking.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Benjamin C. Orsburn, Yuting Yuan, Namandje N. Bumpus
Summary: Single-cell proteomics is a powerful tool for studying cellular heterogeneity and quantifying post-translational modifications. The authors developed a method with improved protein sequence coverage and demonstrated its application in characterizing the effects of inhibitor treatment in single human cells. This approach allows for the detection of multiple classes of post-translational modifications and provides insight into cell-to-cell variability in drug response that is missed by traditional proteomics.
NATURE COMMUNICATIONS
(2022)
Article
Parasitology
Xiaohong Yang, Ningmei Wang, Shuguang Ren, Yuhong Hu, Han Wang, Aimeng Ji, Lihui Cao, Mengxue Li, Jingze Liu, Hui Wang
Summary: This study used quantitative proteomics to investigate the molecular mechanisms of heart damage and self-repair after B. microti infection. The results revealed that proteins involved in coagulation, apoptosis, oxidative phosphorylation, and ribosomes are implicated in the damage and self-repair process. These findings provide valuable insights into the causes of heart disease induced by Babesia infection, and offer potential targets for novel drug development and targeted therapies.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Hui-Ting Huang, Shun-Fen Tzeng
Summary: Our study demonstrates the role of interleukin-33 (IL-33) in a demyelinating mouse model induced by cuprizone (CPZ), showing that IL-33 can alleviate the reduction of APC+ OLs and the decline of IL-33 levels in the corpus callosum, and promote the expression of myelin basic protein (MBP).
NEUROCHEMISTRY INTERNATIONAL
(2024)