Article
Chemistry, Physical
Shixian Cao, Xiaoyuan Tian, Mingyue Cao, Jianguo Wang, Guangle Niu, Ben Zhong Tang
Summary: A series of donor-acceptor-acceptor structured fluorescent acrylonitriles featuring aggregation-induced emission (AIE) were developed for low depletion power STED super-resolution imaging. These materials exhibited tunable near-infrared emissions, high fluorescence quantum yields, significant Stokes shifts, and large two-photon absorption cross-sections. They also showed solvatochromic effect and ultrahigh fluorescence quantum yields in low-polarity solvents.
CHEMISTRY OF MATERIALS
(2023)
Article
Multidisciplinary Sciences
Mariano Gonzalez Pisfil, Iliya Nadelson, Brigitte Bergner, Sonja Rottmeier, Andreas W. Thomae, Steffen Dietzel
Summary: STED microscopy achieves super-resolution by exciting and depleting technique, the phasor based technology can increase the number of fluorochromes in microscopy.
SCIENTIFIC REPORTS
(2022)
Article
Nanoscience & Nanotechnology
Shijie Tu, Xin Liu, Difu Yuan, Wenli Tao, Yubing Han, Yan Shi, Yanghui Li, Cuifang Kuang, Xu Liu, Yufeng Yao, Yesheng Xu, Xiang Hao
Summary: This method improves the signal-to-background ratio in dense sample areas by dynamic phase switching, showing more advantages than software-based background subtraction algorithms.
Article
Optics
Miao Zhao, Fengming Liu, Yang Yu, Xinjun Guo, Hao Ruan, Jing Wen
Summary: This study demonstrates a fast alignment design for spatial matching of two laser beams in an ultra-high resolution microscope. The relative positions of the solid and donut laser beams can be represented using aggregation-induced emission dye resin.
CHINESE OPTICS LETTERS
(2022)
Article
Materials Science, Multidisciplinary
Kwon-Hyeon Kim, Hao Yan, Seok-Hyun Yun
Summary: Dyes with aggregation-induced emission (AIE) properties have been studied for their bright luminescence in solid-state aggregates. The research focuses on the stimulated emission of tetraphenylethene-based organoboron AIE dyes in thin films and microcavity lasers. The results show high gain coefficients and the potential for AIE dyes as laser materials.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Lingbo Zhou, Luojia Chen, Sijie Chen, Zeyuan Pu, Meijia Gu, Yin Shen
Summary: This study reports a highly efficient photodynamic therapy for retinoblastoma using an aggregation-induced emission photosensitizer. The therapy selectively damages tumors, minimizes side effects on normal tissue, and provides protection against metastasis. The photosensitizer also reduces tissue inflammation and exhibits excellent biocompatibility.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Liangliang Liang, Ziwei Feng, Qiming Zhang, Thang Do Cong, Yu Wang, Xian Qin, Zhigao Yi, Melgious Jin Yan Ang, Lei Zhou, Han Feng, Bengang Xing, Min Gu, Xiangping Li, Xiaogang Liu
Summary: This study introduces downshifting lanthanide nanoparticles that enable background-suppressed STED imaging in all-NIR spectral bands, providing high resolution and zero photobleaching effects, allowing for high-contrast deep-tissue imaging.
NATURE NANOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Hongxin Zhang, Mengyao Zhao, Istvan M. Abraham, Fan Zhang
Summary: STED nanoscopy has achieved a significant breakthrough in optical resolution, while lanthanide luminescent probes have enabled advancements in super-resolution imaging. Future challenges lie in promoting the application of lanthanide fluorescent probes in super-resolution imaging.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Aikun Liu, Xiuquan Xing, Haojie Cai, Yutian Zeng, Jiaqing Guo, Hao Li, Wei Yan, Feifan Zhou, Jun Song, Junle Qu
Summary: In this study, the researchers compared different types of quantum dots and found that Cd-free InP/ZnSeS QDs have better photobleaching resistance and a smaller size, which can be used to improve the resolution of STED nanoscopy.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Multidisciplinary Sciences
Tianyan Liu, Till Stephan, Peng Chen, Jan Keller-Findeisen, Jingting Chen, Dietmar Riedel, Zhongtian Yang, Stefan Jakobs, Zhixing Chen
Summary: This study reports a mitochondrial inner membrane fluorescent marker, PK Mito Orange (PKMO), which features reduced phototoxicity and high stability, enabling super-resolution recordings of mitochondrial inner membrane dynamics. The PKMO dye is optically orthogonal with green and far-red markers, allowing multiplexed recordings. By using multi-color STED microscopy, interactions between mitochondria and other cellular components can be captured at sub-100 nm resolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Chemistry, Multidisciplinary
Yanzi Xu, Ruohan Xu, Zhi Wang, Yu Zhou, Qifei Shen, Wenchen Ji, Dongfeng Dang, Lingjie Meng, Ben Zhong Tang
Summary: Recent progress on STED fluorophores for super-resolution imaging is outlined, including inorganic fluorophores, fluorescent proteins, organic luminescent materials, aggregation-induced emission (AIE) luminogens, and fluorescent nanoparticles. Characteristics of these aforementioned STED fluorophores are also included and compared to provide a deep understanding of the relationship between the properties in luminescent materials and their performance in STED imaging.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Shoupeng Cao, Yifeng Xia, Jingxin Shao, Beibei Guo, Yangyang Dong, Imke A. B. Pijpers, Zhiyuan Zhong, Fenghua Meng, Loai K. E. A. Abdelmohsen, David S. Williams, Jan C. M. van Hest
Summary: The study developed biodegradable polymers that can self-assemble into polymersomes for delivering inherent fluorescence, enhancing therapeutic effects. These polymersomes also possess mitochondrial targeting ability, effectively improving the treatment of cancer cells with co-encapsulated drugs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Multidisciplinary
Ning Wang, Yumeng Hao, Xiaowei Feng, Haidan Zhu, Dazhi Zhang, Ting Wang, Xiaoyan Cui
Summary: Stimulated emission depletion (STED) nanoscopy is an essential tool in super-resolution microscopy for revealing substructure and bioactivities in fluorescence imaging. Carboxy-SiR, one of the extensively used STED fluorophores, offers reliable photostability, cell permeability, tunable fluorogenicity, feasible structural decoration, and more. This review provides an overview of the research on carboxy-SiR in STED nanoscopy, aiming to inspire further efforts in the design and application of STED fluorophores.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Zheng Lv, Zhongwei Man, Hongtu Cui, Zhenzhen Xu, Huanhuan Cao, Shuai Li, Qing Liao, Qihua He, Lemin Zheng, Hongbing Fu
Summary: This study presents two efficient red-emitting aggregation-induced emission (AIE) luminogens with off-on fluorescence characteristic for specific lysosomes and mitochondria targeting. The AIE luminogens show good biocompatibility, excellent photostability, and a Stokes' shift larger than 100 nm, enabling high fidelity imaging of lysosomes and mitochondria. Moreover, dynamic lysosomal fusion and mitochondrial fission are imaged with a high resolution of 65.6 nm using stimulated emission depletion (STED) imaging. Additionally, interactions between lysosomes and mitochondria during mitophagy are recorded, providing practical guidance for designing specific organelle targeting probes for live cell dynamic super-resolution imaging.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Physical
Xinrui Yang, Liying Jin, Yuheng Chen, Xiuli Zhong, Yuliang Jiang, Zhihui Dai
Summary: Monitoring hypochlorite levels at the organelle level is crucial for early disease diagnosis. A novel coumarin-based aggregation-induced emission probe (AIE-CDs) was developed for rapid and sensitive detection of hypochlorite, showing potential for biological applications through monitoring and imaging in living cells and zebrafish.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Luyi Zong, Hequn Zhang, Yaqin Li, Yanbin Gong, Dongyu Li, Jiaqiang Wang, Zhe Wang, Yujun Xie, Mengmeng Han, Qian Peng, Xuefeng Li, Jinfeng Dong, Jun Qian, Qianqian Li, Zhen Li
Article
Chemistry, Multidisciplinary
Ji Qi, Chaowei Sun, Dongyu Li, Hequn Zhang, Wenbin Yu, Abudureheman Zebibula, Jacky W. Y. Lam, Wang Xi, Liang Zhu, Fuhong Cai, Peifa Wei, Chunlei Zhu, Ryan T. K. Kwok, Lina L. Streich, Robert Prevedel, Jun Qian, Ben Zhong Tang
Article
Chemistry, Multidisciplinary
Ming Chen, Weisi Xie, Dongyu Li, Abudureheman Zebibula, Yalun Wang, Jun Qian, Anjun Qin, Ben Zhong Tang
CHEMISTRY-A EUROPEAN JOURNAL
(2018)
Article
Pharmacology & Pharmacy
Xinyuan Zhao, Yifan Wu, Jinlong Li, Dongyu Li, Yang Jin, Piaoyu Zhu, Yingqi Liu, Yin Zhuang, Shali Yu, Weiming Cao, Haiyan Wei, Xiaoke Wang, Yu Han, Gang Chen
Article
Optics
Huwei Ni, Zicong Xu, Dongyu Li, Ming Chen, Ben Zhong Tang, Jun Qian
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2019)
Article
Chemistry, Multidisciplinary
Zheng Zheng, Dongyu Li, Zhiyang Liu, Hui-Qing Peng, Herman H. Y. Sung, Ryan T. K. Kwok, Ian D. Williams, Jacky W. Y. Lam, Jun Qian, Ben Zhong Tang
ADVANCED MATERIALS
(2019)
Article
Environmental Sciences
Liling Su, Longtao Zhu, Zhenchao Liu, Jianyao Lou, Bing Han, Chen Lin, Dongyu Li, Jun Qian, Xinyuan Zhao, Guangdi Chen
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Article
Biochemical Research Methods
Dong-Yu Li, Zheng Zheng, Ting-Ting Yu, Ben-Zhong Tang, Peng Fei, Jun Qian, Dan Zhu
JOURNAL OF BIOPHOTONICS
(2020)
Article
Chemistry, Multidisciplinary
Dongyu Li, Hequn Zhang, Lina L. Streich, Yalun Wang, Ping Lu, Ling Wang, Robert Prevedel, Jun Qian
Summary: The use of organic AIE nanoparticles with high absorption cross-section has enabled ultra-deep NIR-IIa excited three-photon imaging in the in vivo mouse brain.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Multidisciplinary
Dongyu Li, Dan Wang, Xinyuan Zhao, Wang Xi, Abudureheman Zebibula, Nuernisha Alifu, Jian-Feng Chen, Jun Qian
MATERIALS CHEMISTRY FRONTIERS
(2018)
Article
Chemistry, Multidisciplinary
Dongyu Li, Wei Qin, Bin Xu, Jun Qian, Ben Zhong Tang
ADVANCED MATERIALS
(2017)
Article
Chemistry, Multidisciplinary
Nuernisha Alifu, Xiaobiao Dong, Dongyu Li, Xianhe Sun, Abudureheman Zebibula, Deqing Zhang, Guanxin Zhang, Jun Qian
MATERIALS CHEMISTRY FRONTIERS
(2017)
Article
Chemistry, Multidisciplinary
Ji Yang, Haoqi Tan, Dongyu Li, Tao Jiang, Yuting Gao, Bo Li, Xue Qu, Jianli Hua
Article
Chemistry, Multidisciplinary
Haiyan Tian, Dongyu Li, Xi Tang, Yubo Zhang, Zhiyong Yang, Jun Qian, Yong Qiang Dong, Mei Han
MATERIALS CHEMISTRY FRONTIERS
(2020)