Article
Biochemistry & Molecular Biology
Filipa B. Pimentel, Marlene Machado, Maria Cermeno, Thanyaporn Kleekayai, Susana Machado, Andreia M. Rego, Maria H. Abreu, Rita C. Alves, Maria Beatriz P. P. Oliveira, Richard J. FitzGerald
Summary: The conchocelis life cycle stage of P. dioica is an unexplored source of bioactive compounds. A specific combination of proteases was used to generate and characterize hydrolysates of conchocelis for the first time, showing improved antioxidant activity and potential as a novel source of antioxidant ingredients.
Article
Plant Sciences
Felisa Rey, Paulo Cartaxana, Sonia Cruz, Tania Melo, M. Rosario Domingues
Summary: Marine algae are important sources of high-value compounds. This study focused on the lipidome and pigment profile of Acetabularia acetabulum, a macroscopic green single-celled alga. Analysis identified 16 polar lipid classes and 191 lipid species, as well as carotenoids and chlorophylls. The lipid extracts showed high antioxidant activity.
JOURNAL OF PHYCOLOGY
(2023)
Article
Chemistry, Medicinal
Diana Lopes, Felisa Rey, Miguel C. Leal, Ana I. Lillebo, Ricardo Calado, Maria Rosario Domingues
Summary: Complex lipids from seaweeds have important phytochemical properties, but their full potential is yet to be fully explored. Current research is mainly based on empirical knowledge, showing the potential application value of seaweed lipids in the food, cosmetics, and pharmaceutical industries.
Article
Food Science & Technology
Ana S. P. Moreira, Elisabete da Costa, Tania Melo, Diana Lopes, Adriana C. S. Pais, Sonia A. O. Santos, Barbara Pitarma, Madalena Mendes, Maria H. Abreu, Pi Nyvall Collen, Pedro Domingues, M. Rosario Domingues
Summary: This study compared commercial Ulva spp. from France and Portugal in terms of composition, esterified fatty acids, and polar lipids, finding significant differences in nutrient content and molecular composition between the two sources.
Article
Chemistry, Medicinal
Tania Melo, Ana R. P. Figueiredo, Elisabete da Costa, Daniela Couto, Joana Silva, M. Rosario Domingues, Pedro Domingues
Summary: This study found that utilizing different solvents to extract polar lipids from N. oceanica can impact the lipid composition of the final extracts. Using ethanol in combination with ultrasonic technologies can improve lipid extraction efficiency and antioxidant activity, with the potential to develop high-added-value biotechnological applications.
Article
Agriculture, Multidisciplinary
Svenja Schneider, Simon Hammann, Heiko Hayen
Summary: A comprehensive analysis of polar lipids in whole grain wheat and oat was conducted, resulting in the identification of 67 lipid species belonging to nine polar lipid classes. The relative composition of four specific lipid classes was investigated in detail.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Elisabete da Costa, Tania Melo, Mariana Reis, Pedro Domingues, Ricardo Calado, Maria Helena Abreu, Maria Rosario Domingues
Summary: The study identified the polar lipid profile of Grateloupia turuturu using modern lipidomics approaches, revealing its richness in EPA and PUFAs, as well as its antioxidant and anti-inflammatory activities. Grateloupia turuturu was shown to be a valuable source of protein and lipids with potential applications in various industries.
Article
Plant Sciences
Vasiliy V. Nokhsorov, Lyubov Dudareva, Svetlana Senik, Nadezhda K. Chirikova, Klim A. Petrov
Summary: The lipid composition of two species of vascular plants in the permafrost zone was studied to reveal significant seasonal trends and changes during cold acclimation. Accumulation of PA and alteration in lipid composition, including a decrease in bilayer lipids and an increase in unsaturation degree, were observed during cold hardening in autumn and winter.
Article
Medicine, Legal
Ana Beatriz Ferreira, Andre Lobo Castro, Sonia Tarelho, Pedro Domingues, Joao Miguel Franco
Summary: An SPE-GC-MS analytical method has been developed and validated for the detection and quantification of various drugs of abuse. The method is specific, selective, and cost-effective, making it suitable for routine forensic analysis.
AUSTRALIAN JOURNAL OF FORENSIC SCIENCES
(2023)
Article
Green & Sustainable Science & Technology
Dmitry D. Evtyugin, Antonio Prates, M. Rosario Domingues, Susana Casal, Dmitry V. Evtuguin
Summary: In this work, a new solvent-free and eco-friendly method for isolating ellagic acid (EA) from sulphite spent liquor and alkali extract of unbleached sulphite pulp is proposed. EA was recovered from sulphite pulping streams via crystallization from acidified solutions under preselected conditions. The purity of EA was significantly increased from approximately 55-70% (w/w) to 85-95% (w/w) through the washing process.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Applied
Luis Gaspar, Fernando Ricardo, Tania Melo, Pedro Domingues, M. Rosario Domingues, Ricardo Calado, Felisa Rey
Summary: The present study performed the first fingerprinting of the polar lipidome of Octopus vulgaris, identifying nearly 400 lipid species from 13 classes of polar lipids. The study highlighted the presence of phosphatidylcholine and phosphatidylethanolamine as the main phospholipids, and ceramides as the main sphingolipids. Additionally, a high proportion of essential fatty acids were found.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Editorial Material
Agriculture, Dairy & Animal Science
Ruben X. G. Silva, Diana Madeira, Paulo Cartaxana, Ricardo Calado
Summary: Climate change-induced bleaching events have become increasingly common in coral reefs. While direct impacts have received attention, the indirect impacts, such as trophic interactions, have been largely overlooked. To address this research gap, the model predator-prey pair of Berghia stephanieae and Exaiptasia diaphana is advocated for studying the trophic effects of bleaching. These organisms are already used in other research fields, allowing for the utilization of existing knowledge on their physiology and culture protocols. The use of this model pair can help unravel the long-term prevalence of trophic effects in coral reef communities.
Article
Chemistry, Analytical
Sara S. Marques, Ines I. Ramos, Carla Silva, Luisa Barreiros, Maria R. Domingues, Marcela A. . Segundo
Summary: The concentration of nanoparticles (NPs) is crucial for the development and quality control of nanocarriers. This study proposes a miniaturized automated method to measure NPs concentration, which is fast, simple, and suitable for research and quality control operations. The method utilizes the measurement of light scattering caused by NPs passing through an optical path, achieving accurate and efficient quantification of NPs concentration.
ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Tiago Jardim Costa, M. Rosario M. Domingues, Eliana Alves
Summary: Tree nuts and oily fruits are highly consumed worldwide due to their nutritional value and health benefits. However, the lipidomic analysis of these foods is still in its early stages.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Chemistry, Physical
Ana Fernandes, Luisa Cruz-Lopes, Bruno Esteves, Dmitry Evtuguin
Summary: In recent years, nanocellulosic materials have gained significant attention due to their performance in different advanced applications, as well as their biodegradability, availability, and biocompatibility. This review focuses on the production and application of nanocelluloses in advanced materials. Various mechanical, chemical, and enzymatic treatments are discussed, along with their effects on producing nanocellulosic materials. The application of nanocellulose, particularly in triboelectric nanogenerators (TENGs), is highlighted, indicating its potential in revolutionizing self-powered sensors, wearable and implantable electronic components, and other innovative applications.
Article
Forestry
Jose M. S. Matos, Dmitry V. Evtuguin, Antonio P. M. Sousa, Maria Graca V. S. Carvalho
Summary: This study evaluated the effects of treating industrial oxygen-delignified eucalypt kraft pulp with a commercial endo-xylanase on ECF bleaching performance. The treatment resulted in improved bleachability and brightness stability of the pulps, with a reduction in chemical loads. However, it also led to an increase in the chemical oxygen demand of the effluent. Overall, the enzymatic treatment showed potential benefits for pulp bleaching, but its environmental impact needs to be considered.
Article
Polymer Science
Fernanda R. Vieira, Nuno V. Gama, Dmitry V. Evtuguin, Carlos O. Amorim, Vitor S. Amaral, Paula C. O. R. Pinto, Ana Barros-Timmons
Summary: Rigid polyurethane foams (RPUFs) were synthesized using lignin-based polyol obtained through the reaction of kraft lignin with propylene carbonate. The formulations were optimized to achieve low thermal conductivity and low density for lightweight insulation. The resulting bio-based RPUFs exhibited comparable properties to commercial RPUFs and showed potential as a replacement for petroleum-based RPUFs. This study introduces a novel approach using 100% unpurified lignin-based polyol in the production of RPUFs.
Article
Green & Sustainable Science & Technology
Patricia I. F. Pinto, Paula C. R. Pinto, Dmitry V. V. Evtuguin, Falk Liebner
Summary: Competition issues in the food industry have led to the exploration of alternative solutions to replace starch in papermaking. Covalent grafting of quaternary ammonium groups onto Eucalyptus kraft lignin has shown promise in providing cationic products with excellent water solubility. This study evaluated the use of cationic lignin as a partial substitution for cationic starch in papermaking, demonstrating that it does not compromise key paper properties and can be fine-tuned to meet specific requirements.
Article
Plant Sciences
Ana C. R. Resende, Rui Pereira, Claudia Nunes, Sonia Cruz, Ricardo Calado, Paulo Cartaxana
Summary: This study investigated the photosynthetic pigments and carbohydrates of Fucus vesiculosus from six locations in the Ria de Aveiro coastal lagoon. Despite differences in environmental factors, the photosynthetic performance, pigment, and carbohydrate concentrations were similar between locations. The concentration of total carbohydrates averaged 418 mg g(-1) dw, with fucose being the second most abundant neutral sugar. Fucoxanthin concentrations were higher than most brown macroalgae, suggesting the potential for valuable bioactive compounds.
Article
Biochemistry & Molecular Biology
Anna Stasiewicz, Tiago Conde, Agnieszka Gegotek, Maria Rosario Domingues, Pedro Domingues, Elzbieta Skrzydlewska
Summary: The lipid extract from Nannochloropsis oceanica effectively protects human keratinocytes from UVB-induced damage by inhibiting cell death, reducing oxidative enzyme activity and ROS levels, and enhancing antioxidant enzyme activity. It also silences overexpressed membrane transporters caused by UVB radiation and decreases inflammation levels, stabilizing the natural metabolism of skin cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Felisa Rey, Paulo Cartaxana, Sonia Cruz, Tania Melo, M. Rosario Domingues
Summary: Marine algae are important sources of high-value compounds. This study focused on the lipidome and pigment profile of Acetabularia acetabulum, a macroscopic green single-celled alga. Analysis identified 16 polar lipid classes and 191 lipid species, as well as carotenoids and chlorophylls. The lipid extracts showed high antioxidant activity.
JOURNAL OF PHYCOLOGY
(2023)
Article
Oncology
Sandrina Nobrega-pereira, Francisco Santos, Miguel Oliveira Santos, Teresa L. Serafim, Ana Patricia Lopes, Diogo Coutinho, Filipa S. Carvalho, Rosario M. Domingues, Pedro Domingues, Bruno Bernardes de Jesus, Vanessa A. Morais, Sergio Dias
Summary: Most cancer-related deaths are caused by metastases. A study showed that low-density lipoprotein (LDL)-cholesterol, a systemic factor, promotes the migration and invasion of triple-negative breast cancer (TNBC) cells by inducing the accumulation of lipid droplets and the expression of CD36. LDL also increases mitochondrial mass and network spread in migrating cells, leading to the dependence on fatty acids for mitochondrial respiration. Furthermore, LDL treatment increases reactive oxygen species (ROS) production. Blocking CD36 or ROS reverses the LDL-induced cell migration and metabolic adaptations. This research reveals a new vulnerability in metastatic breast cancer driven by LDL-induced reprogramming of mitochondrial metabolism.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Michal Biernacki, Tiago Conde, Anna Stasiewicz, Arkadiusz Surazynski, Maria Rosario Domingues, Pedro Domingues, Elzbieta Skrzydlewska
Summary: This study found that the lipid extract derived from Nannochloropsis oceanica microalgae can mitigate the detrimental effects of UVB radiation on skin cells by increasing the concentration of anti-inflammatory and antioxidant polyunsaturated fatty acids, and reducing the production of pro-inflammatory substances and the expression of receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)