Article
Chemistry, Analytical
Monica Jimenez-Carretero, Ylenia Jabalera, Alberto Sola-Leyva, Maria P. Carrasco-Jimenez, Concepcion Jimenez-Lopez
Summary: The immobilization of enzymes on magnetic nanoparticles has attracted interest in detecting contaminants in water samples due to their magnetic control, concentration, and reuse capabilities. In this study, nanoassemblies composed of inorganic or biomimetic magnetic nanoparticles were developed for the detection of trace amounts of organophosphate pesticides and antibiotics in water. Enzyme immobilization was achieved through electrostatic interaction or covalent bonds, with covalent bonding yielding better results. The optimized nanoassemblies demonstrated the ability to detect pollutants at low concentrations and provided enhanced stability and reuse of the enzymes.
Article
Biochemistry & Molecular Biology
Bipasa Dey, Varsha Panwar, Tanmay Dutta
Summary: By preparing crosslinked enzyme aggregates (CLEAs) using sugars, the reusability, retrieval, and storage stability of laccase can be improved. Sugar-CLEA exhibits higher activity recovery and thermal stability, as well as similar pH stability compared to conventional CLEAs, providing essential properties for a biocatalyst.
PROCESS BIOCHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Yuting Lu, Yibo Song, Lingling Peng, Xiaoping Rao, Kok Bing Tan, Shu-feng Zhou, Guowu Zhan
Summary: Metal-organic frameworks (MOFs) have shown potential as host materials for enzyme immobilization and protection. In this study, ZIF-8nanocubes were self-assembled onto yeast as a biological template to obtain hybrid Y@ZIF-8. The size, morphology, and loading efficiency of the assembled ZIF-8 nanoparticles on yeast templates could be controlled by adjusting synthetic parameters. The use of a cross-linking agent greatly enhanced the relative enzyme activity of Y@ZIF-8@t-CAT and improved its cycling stability.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Tobias Heinks, Nicolai Montua, Michelle Teune, Jan Liedtke, Matthias Hoehne, Uwe T. Bornscheuer, Gabriele Fischer von Mollard
Summary: This study aims to compare the stability of soluble and immobilized transaminases from different species. Immobilization of transaminases enabled their reusability for at least 10 cycles and maintained full or high activity. Upscaled kinetic resolutions demonstrated high conversion, maintained enantioselectivity, and high product yields, showing their applicability.
Article
Polymer Science
Evren Sel, Ahmet Ulu, Burhan Ates, Suleyman Koytepe
Summary: In this study, P(MMA-co-PEG500MA) copolymers were successfully utilized for CAT enzyme immobilization, with P3 sample selected as the model support achieving a high immobilization yield of 76%. The immobilized enzyme exhibited improved tolerance and stability, showcasing good reuse potential.
Review
Food Science & Technology
Vashishtha B. Patel, Somak Chatterjee, Abhishek S. Dhoble
Summary: Pectinase enzymes are biological compounds that degrade pectic compounds in fruit juices, making them excellent biocatalysts for juice clarification. However, the poor stability and nonreusability of pectinases limit their effectiveness. Immobilization techniques, particularly using membranes, have been shown to enhance the performance of enzymes. This review highlights the importance of physicochemical properties and kinematic properties of pectinases in relation to juice processing parameters and discusses the potential of membrane immobilization as a support for pectinase.
JOURNAL OF FOOD SCIENCE
(2022)
Review
Agricultural Engineering
Rijuta Ganesh Saratale, Si-Kyung Cho, Ram Naresh Bharagava, Anil Kumar Patel, Sunita Varjani, Sikandar I. Mulla, Dong Su Kim, Shashi Kant Bhatia, Luiz Fernando Romanholo Ferreira, Han Seung Shin, Ganesh Dattatraya Saratale
Summary: Biocatalysts, including live microbial cells/enzymes, have been recognized as a predominant tool for biomass conversion. The use of nanobiocatalysts with different nano-supports can overcome stability and reusability issues, making biomass-based biorefinery profitable and sustainable. This review provides a comprehensive analysis of nanobiocatalyst systems and discusses their applications in waste biomass conversion and commercialization.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Physical
Mozhgan Razzaghi, Ahmad Homaei, Roohullah Hemmati, Dariush Saberi, Soudabeh Kavousipour
Summary: Cellulose nanocrystals/gold nanoparticle hybrids were synthesized using hydrothermal treatment without toxic chemicals. The immobilized enzyme showed increased optimal temperature and retained higher activity and efficiency under extreme conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biotechnology & Applied Microbiology
Gordana Hojnik Podrepsek, Zeljko Knez, Maja Leitgeb
Summary: Immobilized enzymes have higher stability and are easier to use. This study optimized the processes of immobilizing enzymes using CLEAs and mCLEAs methods, and compared the differences between the two methods. The results showed that the activity of immobilized enzymes varied depending on the type of enzyme, and magnetic nanoparticles played an important role in stabilizing the immobilized structure.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hanieh Mohammadi-Mahani, Arastoo Badoei-dalfard, Zahra Karami
Summary: In the study, a purified lipase from Enterobacter sp. MG10 was immobilized using different methods, with CGLNF showing broader activity, higher efficiency, significantly reduced K-m value towards pNPP substrate, and high efficiency in converting prosopis stephanina oil to biodiesel. The results indicate the high potential of CGLNF nanobiocatalyst in synthetic biotechnology, particularly in biodiesel production.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Mengnan Han, Xiaozheng Li, Xuming Wang, Dexu Liu, Shuangqing Fu, Wenzhi Xu, Wei Li, Honglei Zhang
Summary: In this study, a polyhydroxyalkanoate magnetic microsphere was developed for one-step purification and immobilization of a novel carbonyl reductase. The microsphere showed good dispersion and superparamagnetism, and the immobilized enzyme had high activity. The catalyst also maintained its activity after multiple recovery cycles.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Srisakul Trakarnpaiboon, Benjarat Bunterngsook, Hataikarn Lekakarn, Daran Prongjit, Verawat Champreda
Summary: In this study, a cold-active trehalose synthase (PaTreS) was characterized and its properties were investigated. The results showed that the enzyme maintained good activity at lower temperatures and had stability over a broad pH range. Conversion of high-concentration maltose syrup using PaTreS yielded high levels of trehalose with low generation of byproducts.
BIORESOURCES AND BIOPROCESSING
(2023)
Review
Food Science & Technology
Asghar Taheri-Kafrani, Sara Kharazmi, Mahmoud Nasrollahzadeh, Asieh Soozanipour, Fatemeh Ejeian, Parisa Etedali, Hajar-Alsadat Mansouri-Tehrani, Amir Razmjou, Samaneh Mahmoudi-Gom Yek, Rajender S. Varma
Summary: Increasing demand in food and beverage markets due to population growth and health awareness requires improving product quality in a cost-effective and environmentally friendly manner, with the use of enzymes to enhance safety and shelf life. Modification of enzyme characteristics for industrial practices has shown promise in increasing catalytic activity, stability, and reducing costs, although challenges such as enzyme destabilization in harsh industrial conditions persist, necessitating innovative research for solutions.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Review
Biochemistry & Molecular Biology
Shalu Aggarwal, Archana Chakravarty, Saiqa Ikram
Summary: Enzymes are versatile bio-catalysts that can be immobilized on renewable and synthetic supports to enhance catalytic properties and reusability, with recent advancements showing potential applications in pharmaceutical, environmental, and energy sectors. The development of efficient enzyme immobilization systems using organic, inorganic, and nano-based platforms is key to improving enzyme performance and accessibility for diverse applications worldwide.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biophysics
Le Wang, Yu Sha, Yalin Fan, Ying Liang, Dapeng Wu, Qi Wang, Xia Zhang, Rongze Gao, Qipeng Yuan, Jinshui Wang
Summary: In this study, mesoporous polyacrylonitrile (PAN)/thermoplastic polyurethane (TPU) blended nanofibers were prepared to immobilize Micrococcus luteus for enhancing the conversion of trehalose. The immobilized cells not only increased trehalose yield, but also decreased reactive oxygen species levels.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Jingxian Sun, Yixin Yuan, Guoqiang Lu, Tanlong Xue, Jun Nie, Yan Lu
Summary: In this study, a highly stretchable and sensitive strain sensor was successfully fabricated by constructing an ionogel/silver conductive network. By utilizing a transmetalation reaction, the copper atoms in the copper/ionogel nanocomposite were transformed into larger silver atoms, resulting in a silver/ionogel nanocomposite with high conductivity. The conductive ionogel, acting as a polymer substrate, could connect the cracks of the silver conductive network during stretching, leading to high stretchability of the nanocomposite. The obtained strain sensor exhibited high sensitivity in a wide sensing range and could detect various human activities.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Environmental Sciences
Iqra Noor, Hamza Sohail, Jingxian Sun, Muhammad Azher Nawaz, Guohuai Li, Mirza Hasanuzzaman, Junwei Liu
Summary: Heavy metals and metalloids are major soil pollutants that limit crop production worldwide. The exposure to these pollutants induces oxidative stress in plants and alters their biochemical and morphological characteristics. Plants have evolved complex physiological and cellular defense strategies to cope with heavy metal toxicity, such as sequestration and transportation of metals, activation of signaling pathways, and detoxification mechanisms. Understanding the toxicity, signaling pathways, and tolerance mechanisms of heavy metals will help in developing new crop varieties and improving phytoremediation.
Article
Biochemistry & Molecular Biology
Keying Zhu, Yang Wang, Heela Sarlus, Keyi Geng, Erik Nutma, Jingxian Sun, Shin-Yu Kung, Cindy Bay, Jinming Han, Jin-Hong Min, Irene Benito-Cuesta, Harald Lund, Sandra Amor, Jun Wang, Xing-Mei Zhang, Claudia Kutter, Andre Ortlieb Guerreiro-Cacais, Bjorn Hogberg, Robert A. Harris
Summary: This study reveals the promise of TOP1 inhibitors for microglial modulation and highlights the therapeutic strategy of TOP1 inhibition in myeloid cells for neuroinflammatory diseases. The designed nanosystem shows enhanced specificity to myeloid cells and prevents the degradation of the DNA scaffold, offering a potential treatment for diseases with dysfunctional myeloid cells.
Article
Plant Sciences
Xiuxia Yang, Xishan Bai, Hongrui Li, Jingxian Sun, Yong Xiong, Yanhong Li, Xiangzhong Huang
Summary: A phytochemical investigation of Psammosilene tunicoides led to the discovery of a new cyclic peptide compound and three known compounds. The structures of these compounds were elucidated using comprehensive spectroscopic methods, and it was found that some of these compounds exhibited inhibitory activities on the release of NO in cells.
RECORDS OF NATURAL PRODUCTS
(2023)
Article
Nursing
Jingxian Sun, Aihong Wang, Qingqing Xu
Summary: This qualitative study explored the professional identity development experiences of midwifery students in China during clinical placement. The study found that positive experiences can motivate professional identity development, while negative experiences can hinder its formation. These experiences may lead to confusion and decrease students' intention to stay in the profession.
NURSE EDUCATION IN PRACTICE
(2022)
Article
Chemistry, Multidisciplinary
Jing-xian Sun, Ke-ying Zhu, Yu-meng Wang, Dan-jie Wang, Mi-zhen Zhang, Heela Sarlus, Irene Benito-Cuesta, Xiao-qiang Zhao, Zao-feng Zou, Qing-yang Zhong, Yi Feng, Shuai Wu, Yan-qing Wang, Robert A. Harris, Jun Wang
Summary: The role of TRPV1 in demyelinating diseases has been investigated, and it has been found to be an important regulator in the context of demyelination. TRPV1 deficiency exacerbates motor dysfunction and demyelination, while TRPV1 agonist CAP improves behavioral performance and facilitates remyelination. The activation of TRPV1 enhances microglial function and promotes myelin debris clearance.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Immunology
Shanwen Sun, Zhengke Zhi, Yang Su, Jingxian Sun, Qianjun Li
Summary: The study aimed to construct a CD8+ T cell-associated immune gene prognostic model (CDIGPM) for SKCM and explore the immunologic features and the benefits of immunotherapy in CDIGPM-defined SKCM groups.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Jingxian Sun, Yuzi Ge, Xiaobo Gu, Ruyi Li, Wen Ma, Gang Jin
Summary: In this study, 21 high ethanol-tolerance strains of Lactiplantibacillus plantarum were isolated from the eastern foothills of Helan Mountain in Ningxia, China. The strain A7 showed strong resistance to high ethanol and exhibited good malolactic fermentation (MLF) performance, making it a potential new MLF starter culture for high-ethanol wines.
Article
Biochemistry & Molecular Biology
Hongyu Zhang, Danjie Wang, Jingxian Sun, Yumeng Wang, Shuai Wu, Jun Wang
Summary: HupA primarily exerts its therapeutic effects on multiple sclerosis by alleviating demyelination and neuroinflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Wei Tan, Sihua Cheng, Yingying Li, Xiao-Yang Li, Ning Lu, Jingxian Sun, Guiyue Tang, Yujiao Yang, Kezhu Cai, Xuefei Li, Xijun Ou, Xiang Gao, Guo-Ping Zhao, W. Seth Childers, Wei Zhao
Summary: The polarization of distinct scaffold-signaling hubs at opposite cell poles plays a critical role in asymmetric cell division, and phase separation has been shown to be a general mechanism for regulating the assembly and dynamics of a new-pole scaffold-signaling hub.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Haiying Yang, Kaixiang He, Yapu Cao, Zhihao Li, Qiaolin Ji, Jingxian Sun, Ganpeng Li, Xin Chen, Haiying Mo, Gang Du, Qingqing Li
Summary: Ethylene plays an important role in the interaction between Gastrodia elata and Armillaria species. Low concentrations of ethylene significantly improve the mycelium growth of Armillaria, while high concentrations inhibit the growth. Ethylene receptors may exist in Armillaria, and ethylene plays a crucial role in the plant-fungi interaction.
Review
Microbiology
Junwei Fu, Ling Wang, Jingxian Sun, Ning Ju, Gang Jin
Summary: Malolactic fermentation (MLF) is crucial for wine production, but its outcomes are unpredictable and can deteriorate the wine. In recent years, MLF has faced new challenges due to climate change, winemakers' demand for efficiency, and the increasing demand for high-quality wine.
Article
Immunology
Yumeng Wang, Jingxian Sun, Keying Zhu, Danjie Wang, Xiaoqiang Zhao, Hongyu Zhang, Shuai Wu, Yanqing Wang, Jun Wang
Summary: Multiple sclerosis (MS) is an inflammatory-mediated demyelinating disease of the central nervous system (CNS). Microglia with aryl hydrocarbon receptor (AhR) play a crucial role in efficient remyelination and clearance of myelin debris. AhR deficiency in microglia impairs phagocytic function, exacerbates CNS inflammation, and inhibits effective remyelination. This study highlights the therapeutic potential of AhR in demyelinating diseases.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Chemistry, Analytical
Guiyue Tang, Zilong Zhang, Wei Tan, Fei Long, Jingxian Sun, Yingying Li, Siwei Zou, Yujiao Yang, Kezhu Cai, Shenwei Li, Zhiyi Wang, Jiakun Liu, Guobing Mao, Yingxin Ma, Guo-Ping Zhao, Zhen-Gan Tian, Wei Zhao
Summary: Timely and accurate detection of SARS-CoV-2 variants of concern is essential for pandemic surveillance and control. Scientists have developed a nucleic acid testing method, called RRCd, which combines RT-RPA and CRISPR-Cas12a detecting assays to detect and discriminate SARS-CoV-2 mutations. RRCd has high sensitivity, operability, and can provide results within 65 minutes without electrical devices, making it potentially suitable for point-of-care testing of SARS-CoV-2 variants and other epidemic viruses.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Microbiology
Ning Lu, Samuel W. Duvall, Guohong Zhao, Kimberley A. Kowallis, Chao Zhang, Wei Tan, Jingxian Sun, Haley N. Petitjean, Dylan T. Tomares, Guo-Ping Zhao, W. Seth Childers, Wei Zhao
Summary: The direct interaction between PopZ and PodJ has been revealed, which plays a primary role in triggering the bipolarization of PopZ from monopolar during cell development. Disruption of the PodJ-PopZ interaction impairs PopZ-mediated chromosome segregation and positioning.
