Evaluation of Transition Metal Complexes of Benzimidazole-Derived Scaffold as Promising Anticancer Chemotherapeutics

Three new transition metal complexes, Cu(II) 1, Co(II) 2, and Zn(II) 3 with ligand bimnap derived from 1-methyl-2-aminobenzimidazole and 2-hydroxynapthaldehyde were synthesized and characterized. The structure of the ligand was determined by single X-ray crystallography. All the three complexes, 1-3, were examined for the mode of interaction with biomolecule viz., calf thymus-DNA (CT-DNA) using various spectroscopic methods. The nuclease activity was performed against pBR322 DNA that exhibited concentration-dependent degradation of the nucleic acid. The mechanism of DNA cleavage was studied by the electrophoretic pattern in the presence of the radical scavengers. Also, the complexes 1-3 were analyzed for groove binding affinity. Moreover, in vitro cytotoxicities of the complexes 1-3 were tested against the five human cancer cell lines, i.e., HeLa, SK-MEL-1, HepG2, HT108, and MDA-MB 231. Also, the cell adhesion and migration properties upon treatment of cell lines with complexes 1-3, and consequently, their cell death pathway via apoptosis and necrosis were analyzed. Further, complexes 1-3 were studied in vivo for their toxicities and tolerabilities in mice. In sum, the complexes 1-3 showed merits of an effective anticancer agent in cell lines-based study while minor side effects were observed in vivo.