Article
Chemistry, Applied
Michael A. Pitino, Sharon Unger, Alain Doyen, Yves Pouliot, Akash Kothari, Melanie Sergius-Ronot, Richard P. Bazinet, Debbie Stone, Deborah L. O'Connor
Summary: Holder pasteurization (HoP) can lead to denaturation and aggregation of bioactive proteins, while high pressure processing (HPP) minimally affects bioactive components. In vitro digestion showed that HoP increased proteolysis of lactoferrin and milk fat globule membrane proteins. Lysozyme activity was significantly impacted by HoP digestion compared to HPP or raw milk. HPP-treated donor milk is digested similarly to raw milk, while preserving functional bioactive proteins.
Article
Food Science & Technology
Melanie Sergius-Ronot, Shyam Suwal, Michael A. Pitino, Sara Shama, Sharon Unger, Deborah L. O'Connor, Yves Pouliot, Alain Doyen
Summary: The impact of different processing methods on the digestibility of human milk protein concentrate (HMPC) was evaluated. Results showed that heat treatments and high hydrostatic pressure (HHP) affected the release of certain amino acids during digestion. However, overall digestibility was similar between pasteurized and raw HMPC. The non-thermal HHP process was found to preserve the bioactive proteins in HMPC during digestion.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Nutrition & Dietetics
Robert L. Beverly, Robert K. Huston, Andi M. Markell, Elizabeth A. McCulley, Rachel L. Martin, David C. Dallas
Summary: The study found differences in the digestion of milk proteins between preterm and term infants. Preterm infants released more total peptides in the stomach, while term infants released specific bioactive peptides at higher abundance. A region at the C-terminus of b-casein was identified as being conserved from milk through stool and releasing known and potential antimicrobial peptides.
CLINICAL NUTRITION
(2021)
Article
Nutrition & Dietetics
Mariana Muelbert, Tanith Alexander, Mark H. Vickers, Jane E. Harding, Laura Galante, Frank H. Bloomfield
Summary: Glucocorticoids (GCs), including cortisol and cortisone, are regulators of physiological responses and are present in breast milk. This study found that cortisol concentrations in breast milk increased over time, while cortisone concentrations remained stable. The use of antenatal corticosteroids, maternal stress, depression, and obesity were all associated with the concentration of GCs in breast milk.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Applied
Zhengzheng Zou, John A. Duley, David M. Cowley, Sarah Reed, Buddhika J. Arachchige, Pieter Koorts, Paul N. Shaw, Nidhi Bansal
Summary: The absence of beta-lactoglobulin, high beta-/alpha(s)-casein ratio and protective proteins make camel milk a promising alternative protein base for making human infant formulae. In this study, protein digestibility of camel milk was compared with that of bovine and human milk using an in vitro infant gastrointestinal digestion system. The results suggest that camel milk proteins are equally digestible as bovine and human milk proteins under infant gastrointestinal digestion conditions, and it may be a prospective substitute for infant formula base.
Review
Cell Biology
Xue Jiang, Lianghui You, Zhenxing Zhang, Xianwei Cui, Hong Zhong, Xingzhen Sun, Chenbo Ji, Xia Chi
Summary: Extracellular vesicles (EVs) released by cells carry various biomolecules, with a focus on miRNAs in milk-derived EVs. These miRNAs play a regulatory role and have physiological effects on growth and development in both infants and adults. Research on milk-derived EVs is increasing, providing new insights into their potential applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xin-qi Zhong, Qin Yan, Zhuang-gui Chen, Chun-hong Jia, Xiu-hong Li, Zi-yan Liang, Jian Gu, Hui-ling Wei, Chang-yu Lian, Jing Zheng, Qi-liang Cui
Summary: Exosomes derived from umbilical cord blood of BPD infants impair angiogenesis, possibly via differentially expressed EXO-miRNAs, which might contribute to the development of BPD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Nutrition & Dietetics
Michael A. Pitino, Megan R. Beggs, Deborah L. O'Connor, Alain Doyen, Yves Pouliot, Melanie Sergius-Ronot, Sharon Unger
Summary: For preterm infants, pasteurized donor human milk is preferred over formula when there is insufficient supply of mother's milk. However, changes in composition and reduction in bioactivity during processing affect the growth of these infants. Research is currently investigating strategies to optimize processing techniques, but studies on the impact of donor milk processing on infant digestion/absorption are lacking. More research is needed to improve the quality and nutrition of donor milk.(
ADVANCES IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Ting Xiao, Junpeng Zeng, Leyun Qiu, Ruiyan Wang, Nan Li, Zeyuan Deng, Liufeng Zheng
Summary: Potential endogenous hypoglycemic peptides derived from breast milk were screened and validated for their inhibitory effects on glucose uptake. One of the peptides, YPVTQPLAPVHNPIS, showed the most significant inhibitory activity. In vitro digestion and molecular docking experiments confirmed the strong hypoglycemic activity of YPVTQPL.
Article
Biochemistry & Molecular Biology
Xiangjun Zhou, Sheng Zhao, Wei Li, Yuan Ruan, Run Yuan, Jinzhuo Ning, Kun Jiang, Jinna Xie, Xiaobin Yao, Haoyong Li, Chenglong Li, Ting Rao, Weimin Yu, Fan Cheng
Summary: Unilateral ischemia reperfusion injury (UIRI) with longer ischemia time is associated with an increased risk of acute renal injury and chronic kidney disease. Exosomes play a key role in mediating communication between epithelial and mesenchymal cells relevant to renal fibrosis after UIRI. Inhibition of exosome secretion or miRNA activity could be a potential therapeutic target for preventing progression to renal fibrosis following UIRI.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Ningjian Liang, Bum Jin Kim, David C. Dallas
Summary: The study showed that certain milk peptides can cross the intestinal cell monolayer and reach the bloodstream to exert bioactivities.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Yue Pan, Yu Xia, Xiaoxue Yu, Muhammad Hussain, Xiaodong Li, Lu Liu, Lina Wang, Chunmei Li, Youbin Leng, Shilong Jiang
Summary: This study investigated the lipid digestion characteristics in human, bovine, and caprine milk using an infant in vitro digestion model. The results showed significant differences in particle size, lipolysis degree, and fatty acid release between human milk and bovine/caprine milk. A total of 30 and 31 lipids were identified as biomarkers to further clarify the differences in lipid digestion properties of the three types of milk.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Nutrition & Dietetics
Ningjian Liang, Robert L. Beverly, Brian P. Scottoline, David C. Dallas
Summary: The study revealed that bioactive peptides in human milk have immunomodulatory activity after digestion in infants, with some peptides exhibiting anti-inflammatory effects and others proinflammatory effects. These peptides potentially contribute to the observed immunomodulatory activity.
JOURNAL OF NUTRITION
(2022)
Article
Chemistry, Applied
Stefano Nebbia, Amelie Deglaire, Olivia Menard, Gwenaele Henry, Elettra Barberis, Marcello Manfredi, Enrico Bertino, Alessandra Coscia, Didier Dupont, Marzia Giribaldi, Laura Cavallarin
Summary: This study found that an experimental donkey milk-derived fortifier may be a better source of proteins and lipids for preterm infants compared to a commercial bovine milk-derived fortifier.
Article
Environmental Sciences
Maria Krishna de Guzman, Dragana Stanic-Vucinic, Nikola Gligorijevic, Lukas Wimmer, Manvel Gasparyan, Tamara Lujic, Tamara Vasovic, Lea Ann Dailey, Sam Van Haute, Tanja Cirkovic Velickovic
Summary: In this study, the interaction between pepsin and polystyrene microplastics ( MPs) was evaluated. The surface chemistry of MPs was found to determine the binding affinity of pepsin. Prolonged exposure to MPs led to structural changes in pepsin and a significant reduction in its activity during gastric digestion.
ENVIRONMENTAL POLLUTION
(2023)
Article
Entomology
Kay Anantanawat, Alexie Papanicolaou, Kelly Hill, Wei Xu
Summary: Invasive Tephritid fruit flies pose a global threat to agriculture and horticulture industries. Biosecurity has been crucial in reducing their damage, but with the ban of key chemical pesticides, non-chemical approaches such as heat and cold treatments are being utilized more. A recent study found that the molecular response of fruit flies to cold treatment differs from their response to heat, shedding light on potential new strategies for fruit fly management.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Entomology
Basman H. Al-Jalely, Penghao Wang, Yalin Liao, Wei Xu
Summary: This study conducted transcriptome sequencing on the antennae of male and female adult Diadegma semiclausum wasps, revealing differential expression of OBPs and ORs, providing important insights into the olfactory system of D. semiclausum.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Entomology
Li-Jun Cai, Tian-Pu Li, Xi-Jian Lin, Yu-Ping Huang, Jiang-Mei Qin, Wei Xu, Min-Sheng You
Summary: This study identified nine innexin genes from the P. xylostella genome, with Pxylinx4 showing specific expression in eggs and female ovaries, suggesting its role as a candidate zpg gene. RNA interference on Pxylinx4 in female P. xylostella resulted in decreased egg-laying without affecting egg-hatching. These findings provide insights into P. xylostella innexin genes and offer a potential molecular target for controlling this economically important pest.
PHYSIOLOGICAL ENTOMOLOGY
(2022)
Article
Entomology
Aniruddha Agnihotri, Naiyong Liu, Wei Xu
Summary: Chemosensory proteins (CSPs) are small, soluble proteins that play a crucial role in transporting odorant and pheromone molecules in the insect chemosensory system. Recent studies reveal that they also function in development, nutrient metabolism and insecticide resistance. In this study, 27 CSP genes were identified from the genome and transcriptome sequences of cotton bollworm, Helicoverpa armigera (Hubner). The expression patterns of these genes were studied by using transcriptomic data obtained from different tissues and stages. The results demonstrate that H. armigera CSP genes are not only highly expressed in chemosensory tissues, such as antennae, mouthparts, and tarsi, but also in the salivary glands, cuticle epidermis, and hind gut. A new method was developed that significantly increased the expression levels of two selected CSPs as soluble recombinant proteins for purification. This study advances our understanding of insect CSPs and provides a new approach to highly express recombinant CSPs in E. coli.
Article
Entomology
Lei Guo, Zhuang Zhang, Wei Xu, Jiangya Ma, Ni Liang, Changyou Li, Dong Chu
Summary: This study reveals that the P450 gene CYP402C1 is associated with the resistance of Bemisia tabaci to imidacloprid. Knocking down CYP402C1 significantly reduces the resistance of B. tabaci, while overexpressing CYP402C1 in transgenic Drosophila melanogaster increases resistance. Heterologous expression of CYP402C1 shows its ability to metabolize imidacloprid.
Article
Biochemistry & Molecular Biology
Lijun Cai, Sarina Macfadyen, Baozhen Hua, Haochuan Zhang, Wei Xu, Yonglin Ren
Summary: Monitoring and early detection of stored-grain insect infestation is crucial for effective pest management. This study presents a reliable method using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry to assess insect infestation in stored grains. Specific volatile compounds emitted by the infested grains were identified and can be utilized for insect surveillance and monitoring in stored grain.
Editorial Material
Physiology
Peng He, Yang Liu, J. Joe Hull, Ya-Nan Zhang, Jin Zhang, Xiao-Jiao Guo, Wei Xu
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Entomology
Lijun Cai, Sarina Macfadyen, Baozhen Hua, Wei Xu, Yonglin Ren
Summary: This study analyzed volatile chemical compounds released from the granary weevil Sitophilus granarius and identified two key compounds, 3-hydroxy-2-butanone and 1-pentadecene, which played important roles in the electrophysiological and behavioral responses of this insect. These compounds have potential in developing new approaches for monitoring and managing insect pests in stored grain, reducing postharvest loss.
Article
Biochemistry & Molecular Biology
Zhao Liu, Qian-Ping Xie, Huai-Wang Guo, Wei Xu, Jin-Jun Wang
Summary: In this study, the antenna-specific OBP gene from Bactrocera dorsalis was cloned and found to have different functions in male and female flies. The RNAi injection experiment and behavioral bioassay confirmed its involvement in the detection of methyl eugenol in males and gamma-octalactone in females.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Multidisciplinary
Zhuang Zhang, Zanrong Wen, Kaixin Li, Wei Xu, Ni Liang, Xinyue Yu, Changyou Li, Dong Chu, Lei Guo
Summary: Bemisia tabaci is a significant agricultural pest that demonstrates resistance to various insecticides. This study highlights the involvement of CYP6CX3 in the resistance to cyantraniliprole in B. tabaci.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Shovon Chandra Sarkar, Stephen Paul Milroy, Wei Xu
Summary: The variegated lady beetle, Hippodamia variegata, shows potential as a biocontrol agent for the invasive pest, Bactericera cockerelli, and may contribute to improving current management strategies.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Kaixin Li, Jiantao Liu, Ziqiong Geng, Wei Xu, Zhuang Zhang, Dong Chu, Lei Guo
Summary: This study identified a highly resistant Bemisia tabaci population to dinotefuran, a new neonicotinoid insecticide. The resistance was found to be polygenic and incompletely recessive. The study revealed the involvement of P450 enzymes, particularly the CYP6DW5 gene, in the resistance mechanism. This information is important for resistance management in B. tabaci.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Shovon Chandra Sarkar, Stephen Paul Milroy, Wei Xu
Summary: The present study examined the Australian native ladybird beetle Coccinella transversalis as a potential biological control agent of the tomato potato psyllid Bactericera cockerelli. The results showed that C. transversalis could successfully survive, develop, and reproduce when fed B. cockerelli as a monotypic diet. In a glasshouse trial, C. transversalis larvae reduced the population growth of B. cockerelli and increased plant biomass.
BIOLOGICAL CONTROL
(2022)
Article
Agronomy
Zanrong Wen, Jinzheng Feng, Bolin Zhu, Wei Xu, Fa Xu, Huiwen Tan, Dong Chu, Lei Guo
Summary: This study discovered a new method to prevent the transmission of Tomato chlorosis virus by using the new pesticide pyrifluquinazon, which can effectively reduce the ability of whitefly to transmit the virus. This finding is of great significance for controlling tomato yellow leaf disorder.
PEST MANAGEMENT SCIENCE
(2023)
Review
Entomology
Shovon Chandra Sarkar, Severin Hatt, Andrew Philips, Mahjuba Akter, Stephen Paul Milroy, Wei Xu
Summary: The tomato potato psyllid is a major insect pest of potato, tomato, and other solanaceous crops. First detected in Australia in 2017, it poses a significant threat to the vegetable industry. The insect damages crops directly through feeding and indirectly as a vector of a pathogen causing zebra chip disease in potatoes. Biological control with native natural enemies may be a viable alternative strategy to manage this insect.