Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 62, Issue 9, Pages -Publisher
WILEY
DOI: 10.1002/mnfr.201701007
Keywords
beta-oxidation; delta-desaturases; diabetes type 1; fatty acids; lipid oxidation; RAGE
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Funding
- Croatian Science Foundation [IP-2014-09-8992]
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Scope: We investigated the interaction between streptozotocin (STZ)-induced diabetes and dietary n6/n3 ratio, and its influence on lipogenesis. Methods and results: The animals were treated with STZ and fed with different dietary n6/n3 ratios: 1, 7, and 60, or supplemented with DHA/EPA. Gene expression was assessed by RT-PCR and protein expression by western blotting and immunohistochemistry. Fatty acid profile was determined by GC-MS. Pancreas and liver histology were assessed by hematoxylin and eosin (H&E) staining. STZ-induced characteristic changes in all STZ treated groups, including: increased blood glucose, decreased body mass, increased lipid peroxidation and CD36 expression, decreased 16:1n7 and 18:1n7, increases in 20:3n6, decreases in phospholipid (PL) content of 20:4n6, as well as decreases in the expression of SREBP1c, Delta-9-desaturase (Delta 9D), and Delta-5-desaturase (Delta 5D). Additionally, other changes occurred that were dependent on the n6/n3 ratio. Among the diabetic groups, the lower n6/n3 ratio caused higher lipid peroxidation and CD36 expression, a greater decrease in 20:4n6 and decreased Delta 6-desaturase (Delta 6D) expression, while the higher n6/n3 ratio caused increased partitioning of 20:4n6 into hepatic neutral lipids (NL), a decrease in 20:5n3 content, and increased beta-oxidation. Conclusion: Presented data suggest that the n6/n3 ratio could significantly influence lipogenesis, lipid peroxidation, and beta-oxidation in STZ-induced diabetes, which could have clinical significance.
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