Journal
MOLECULAR CANCER RESEARCH
Volume 16, Issue 10, Pages 1568-1578Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1541-7786.MCR-18-0120
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Funding
- Department of Medicine for the Division of Matrix Biology at Beth Israel Deaconess Medical Center
- NIH [CA125550]
- Cancer Prevention and Research Institute of Texas
- University of Texas MD Anderson Cancer Center Khalifa Bin Zayed Al Nahya Foundation
- University of Bergen, Norway
- Fulbright Association
- Helse Vest, Norway
- Stop and Shop Pediatric Brain Tumor Foundation
- NATIONAL CANCER INSTITUTE [P30CA016672, R01CA125550] Funding Source: NIH RePORTER
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Deregulated transforming growth factor-beta (TGF beta) signaling is a common feature of many epithelial cancers. Deletion of TGF beta receptor type 2 (TGF beta R2) in fibroblast specific protein-1 (FSP1)-positive stromal cells induces squamous cell carcinoma in the murine forestomach, implicating fibroblast-derived hepatocyte growth factor (HGF) as the major driver of the epithelium carcinogenesis. Prior to cancer development, hyperproliferative FSP1 thorn fibroblasts lacking TGF beta R2 accumulate in the forestomach, disrupting the regulatory signaling cross-talk with the forestomach epithelium. Here, concurrent loss in TGF beta R2 and SMAD4 completely abrogates the development of forestomach cancer. Bone morphogenic protein-7 (BMP7) was highly upregulated in forestomach cancer tissue, activating Smad1/5/8 signaling, cell proliferation, and HGF production in TGFBR2-deficient FSP1 thorn fibroblasts. This stimulation by BMP7 was lost in the combined TGFBR2 and SMAD4 double knockout fibroblasts, which included a profound decrease in HGF expression. Thus, Smad4-mediated signaling is required to initiate epithelial carcinogenesis subsequent to TGFBR2 deletion in FSP1 thorn fibroblasts. Implications: These findings reveal a complex cross-talk between epithelial cells and the stroma, wherein Smad4 is required to elicit squamous cell carcinomas in the forestomach of mice with TGFBR2-deficient stromal cells. (C) 2018 AACR.
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