Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 29, Issue 16, Pages 1975-1991Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E18-01-0056
Keywords
-
Categories
Funding
- National Center for Research Resources, National Institutes of Health
- National Institutes of Health National Institute of General Medical Sciences [GM110268]
- Israel Science Foundation [1293/17]
Ask authors/readers for more resources
Productive and coordinated tissue contraction requires spatiotemporal regulation of myosin activity. We use the contractile myoepithelial cells of the Caenorhabditis elegans spermatheca to elucidate the molecular mechanisms involved in contraction. Here, we identify and describe a novel myosin light-chain kinase, MLCK-1, that phosphorylates the myosin regulatory light chain and is required for contraction of the spermatheca and animal fertility. During contraction, MLCK-1 is recruited to basal actomyosin bundles and stabilizes myosin in these bundles downstream of phospholipase PLC-epsilon/PLC-1 and calcium signaling. MLCK and the Rho kinase ROCK are expressed in distinct subsets of spermathecal cells and act in concert to coordinate the timing of contraction. Our results suggest that MLCK-1 phosphorylates myosin primarily in the central bag cells of the spermatheca, while ROCK controls contractility in the distal neck and the valve connecting the spermatheca to the uterus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available