Journal
MICROCHIMICA ACTA
Volume 185, Issue 3, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2734-5
Keywords
BSA@AuNCs; BSA@AuNC-Cu(II) complexes; PPi; Telomerase activity; dNTPs; HeLa cells; Fluorometric assay; Cell imaging
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Funding
- National Natural Science Foundation of China [21375070]
- Fund Project for Shangdong Key RD Program [2010GGX10417]
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Gold nanoclusters (AuNCs) protected with a bovine serum albumin (BSA) coating are known to emit red fluorescence (peaking at 650 nm) on photoexcitation with ultraviolet light (365 nm). On addition of Cu(II) ions, fluorescence is quenched because Cu(II) complexes certain amino acid units in the BSA chain. Fluorescence is, however, restored if pyrophosphate (PPi) is added because it will chelate Cu(II) and remove it from the BSA coating on the AuNCs. Because PPi is involved in the function of telomerase, the BSA@AuNCs loaded with Cu(II) can act as a fluorescent probe for determination of the activity of telomerase. A fluorescent assay was worked out for telomerase that is highly sensitive and has a wide linear range (10 nU to 10 fM per mL). The fluorescent probe was applied to the determination of telomerase activity in cervix carcinoma cells via imaging. It is shown that tumor cells can be well distinguished from normal cells by monitoring the differences in intracellular telomerase activity.
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