Journal
MICROCHIMICA ACTA
Volume 185, Issue 8, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2884-5
Keywords
Aptasensor; Pt@Au nanoparticles; Cyclic voltammetry; Electrochemical impedance spectroscopy; Signal amplification; Electrochemical detection; Effective surface area
Categories
Funding
- National Natural Science Foundation of China [81601856]
- Third Batch of Young Backbone Teachers Funding Program in Colleges and Universities of Chongqing City [53]
- Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City [cstc2014kjrc-qnrc00004]
- Fundamental and Advanced Research Projects of Chongqing City [cstc2016jcyjA0275]
Ask authors/readers for more resources
The 6-kDa early secretory antigenic target referred to as ESAT-6 is a virulence factor secreted by Mycobacterium tuberculosis (MTB). This work describes a voltammetric aptasensor for ultrasensitive detection of ESAT-6. Reduced graphene oxide doped with metal-organic framework (MOF-rGO) was deposited on a glassy carbon electrode (GCE). This increases the immobilization of electroactive Toluidine Blue (TB) and facilitates the electron transfer from TB to the modified GCE. Platinum/gold core/shell (Pt@Au) nanoparticles were used to assemble thiolated ESAT-6 binding aptamer (EBA) on a modified electrode and to further amplify the response to TB. The modified GCE, typically operated at -0.36 V (vs. SCE), has a linear response in 1.0 x 10(-4) to 2.0 x 10(2) ngai...mL(-1) ESAT-6 concentration range, and the limit of detection (LOD) for ESAT-6 is as low as 3.3 x 10(-5) ngai...mL(-1). It exhibits satisfactory specificity and reproducibility when analyzing spiked human serum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available