Journal
MICROCHIMICA ACTA
Volume 185, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-2703-z
Keywords
Single gold nanowire electrodes; Electrochemical DNA nanosensor; Methylene blue; Square wave voltammetry
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Funding
- National Natural Science Foundation of China [21775003, 21375002]
- Doctoral Scientific Foundation Project of the Ministry of Education of China [20133227120002]
- Foundation for Innovation Team of Bioanalytical Chemistry of Anhui Province
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The authors describe an electrochemical DNA nanosensor based on the use of single gold nanowire electrodes (AuNWEs). The probe DNA is immobilized on the AuNWE via Au-S bonds that are formed between thiol-terminated DNA and the gold surface. Single AuNWEs were prepared by an improved laser-assisted pulling method and hydrofluoric acid etching. The nanoelectrodes were characterized by cyclic voltammetry and COMSOL simulation. Square wave voltammetry was used to monitor the DNA hybridization event between probe DNA and target DNA by using Methylene Blue (MB) as an intercalator of dsDNA. Under optimal conditions, the peak current for MB (best measured at a potential of -0.2 V vs. Ag/AgCl) increases linearly with the logarithm of the analyte concentration in the 1.0 f. to 10 nM range, with a 0.48 fmM detection limit at an S/N ratio of 3. The assay is highly selective, reproducible and stable. Considering the small overall dimensions and high sensitivity, this nanoelectrode potentially can be applied to in-vivo sensing of DNA inside living cells
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