Journal
MEDICAL AND VETERINARY ENTOMOLOGY
Volume 32, Issue 3, Pages 358-364Publisher
WILEY
DOI: 10.1111/mve.12306
Keywords
Chagas' disease vector; entomopathogenic fungi; gene expression; triatomine bugs
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Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [CAPES (Agency for Support and Evaluation of Postgraduate Education)], Brazil
- National Agency for Science and Technology Promotion in Argentina [PICT 2012 1964]
- CAPES (Brazil) [PCPP 016/2011]
- SPU (Argentina) [PCPP 016/2011]
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Physically disturbed Triatorna infestans (Hemiptera: Reduviidae) adults, as well as adults of other Chagas' disease vectors, secrete a mix of volatile organic compounds (VOCs) with alarm and possible sexual and defence functions. The aim of the present research was to test whether infection with the entomopathogenic fungus Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae) has an effect on VOC secretion in disturbed T. infestans and on the expression of two genes (Ti-brnq and Ti-bckdc) potentially involved in VOC biosynthesis. The volatilcs released by insects at different time periods after fungal treatment were identified and their relative amounts measured. Isobutyric acid was the most abundant volatile found in both healthy and fungus-infected insects and underwent no significant relative changes through the infection process. The secretion of propionic acid, however, was significantly higher at 1-4 days post-infection (d.p. i.) compared with that in controls. A slight induction of both Ti-brnq and Ti-bckdc genes was found by real-time polymerase chain reaction at 4 d.p. i., with expression values reaching up to three-fold those in controls. The early stages of fungal infection seem to affect the composition of the alarm pheromone by changing the expression pattern of both genes analysed. These results help to elucidate the impact of fungal infections on the chemical ecology of triatomine bugs.
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