Article
Biotechnology & Applied Microbiology
Hae Sang Park, Ji Seung Lee, Chang-Beom Kim, Kwang-Ho Lee, In-Sun Hong, Harry Jung, Hanna Lee, Young Jin Lee, Olatunji Ajiteru, Md Tipu Sultan, Ok Joo Lee, Soon Hee Kim, Chan Hum Park
Summary: In this study, we successfully created a three-dimensional bioprinted artificial larynx using rabbit's chondrocyte-laden gelatin methacryloyl (GelMA)/glycidyl-methacrylated hyaluronic acid (GMHA) hybrid bioink. We incorporated a polycaprolactone (PCL) outer framework with pores to provide structural strength and a suitable microenvironment for the printed cells. Our novel fluidics supply (FS) system improved cell viability during the printing process, leading to the generation of a large-scale cell-laden artificial laryngeal framework. Animal studies confirmed the functionality of the transplanted 3D bio-larynx, offering potential for clinical applications in laryngectomy patients.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Lucy A. Bosworth, Kyle G. Doherty, James D. Hsuan, Samuel P. Cray, Raechelle A. D'Sa, Catalina Pineda Molina, Stephen F. Badylak, Rachel L. Williams
Summary: The conjunctiva, a critical tissue for eye protection and homeostasis, can be impaired by diseases requiring surgical intervention. A tissue engineering approach using PCL and UBM scaffolds showed potential for inducing stratification of human conjunctival epithelial cells. Further investigation is needed for these matrix-containing scaffolds as a potential therapy for conjunctival repair and regeneration.
Article
Materials Science, Multidisciplinary
Kenichi Nagase, Ruka Shimane
Summary: This study developed cell separation materials using ethylene glycol-based thermoresponsive block copolymer brushes and cell-affinity peptides, which can separate cells through temperature changes. These materials have the potential to play an important role in tissue engineering applications.
MATERIALS & DESIGN
(2023)
Article
Engineering, Biomedical
Maria Maqueda, Jose Luis Mosquera, Jose Garcia-Arumi, Anna Veiga, Anna Duarri
Summary: Decellularized retinas from mouse and pig contain common native ECM components that can guide similar ocular cell adhesion, migration, and organization upon cell repopulation.
Article
Medicine, Research & Experimental
Mohammed Imran Khan, Meena Easwaran, Joshua D. Martinez, Akari Kimura, Elizabeth Erickson-DiRenzo
Summary: The objective of this study was to develop a method for harvesting individual epithelial cells from the mouse larynx, while minimizing contamination from non-laryngeal sites and non-epithelial laryngeal cells. The results showed successful collection of a large number of epithelial cells, and confirmed that the harvested cells were primarily epithelial. This method will be useful for downstream cellular and molecular assays.
Article
Ophthalmology
Kanika Bhardwaj, Uma Sridhar
Summary: The shortage of donor corneal tissue has prompted research on tissue engineering methods to create alternatives. In this study, a biodegradable scaffold was developed using a mixture of PDLLA and Eudragit polymers. The scaffold showed stability, porosity, and water retention capacity, making it suitable for in vitro growth of corneal epithelial stem cells. The scaffold made with a 70:30 ratio of PDLLA to Eudragit was found to promote the growth of laboratory corneal epithelial stem cells.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Olimpia Ortiz-Arrabal, Jesus Chato-Astrain, Pascual Vicente Crespo, Ingrid Garzon, Maria Dolores Mesa-Garcia, Miguel Alaminos, Carolina Gomez-Llorente
Summary: In this study, the potential of maslinic acid (MA) to improve keratinocyte culture methods for skin tissue engineering was evaluated. The results showed that MA increased cell proliferation and activity without affecting cell viability. Additionally, MA upregulated gene expression and enhanced cell dissociation and explant efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Xiaoya An, Guoliang Wang, Mengyi Jin, Xiaoping Zhou, Shubin Gao, Jingyao Chen, Peter S. Reinach, Zuguo Liu, Yuhua Xue, Cheng Li
Summary: The novel 6C medium developed in this study contains six different signaling pathway modulators to control the proliferative activity of mouse corneal epithelial cells, which helps expedite the healing of epithelial wounds in experimental animal models. By inhibiting specific markers of epithelial mesenchymal transdifferentiation, this medium facilitates the procurement of epithelial progenitor cells in a feeder-free system, ultimately improving cell fate determination and potentially enhancing outcomes in corneal regenerative medicine.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Gustavo de Sa Schiavo Matias, Ana Claudia O. Carreira, Vitoria Frias Batista, Hianka Jasmyne Costa de Carvalho, Maria Angelica Miglino, Paula Fratini
Summary: Decellularized extracellular matrix can be used as a biomaterial for tissue engineering, particularly for partial trachea replacement. In this study, acellular canine tracheal scaffolds with preserved ECM structure and proteins were obtained and evaluated for cell adhesion and proliferation. The results showed successful recellularization of the scaffolds with canine tracheal epithelial cells and yolk sac endothelial progenitor cells. In vivo implantation in mice confirmed the preserved tissue and absence of fibrotic tissue process. This study demonstrates the potential of recellularized scaffolds for future applications in tissue engineering.
Review
Biochemistry & Molecular Biology
Francesca Camponogara, Federica Zanotti, Martina Trentini, Elena Tiengo, Ilaria Zanolla, Elham Pishavar, Elisa Soliani, Marco Scatto, Paolo Gargiulo, Ylenia Zambito, Stefano De Luca, Letizia Ferroni, Barbara Zavan
Summary: Regenerative medicine utilizes stem cell therapy and tissue engineering strategies to guide the healing or replacement of damaged tissues or organs. Polymers play a crucial role in this field as they can be used as principal components, coatings, or vehicles to functionalize various biomaterials. This review provides an overview of the current state of polymer research in Italy for regenerative medicine, highlighting the importance for the development of novel biomaterials and strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Polymer Science
Antony V. V. Samrot, Mahendran Sathiyasree, Sadiq Batcha Abdul Rahim, Robinson Emilin Renitta, Kasirajan Kasipandian, Sivasuriyan Krithika Shree, Deenadhayalan Rajalakshmi, Nagarajan Shobana, Shanmugaboopathi Dhiva, Sasi Abirami, Sridevi Visvanathan, Basanta Kumar Mohanty, Gokul Shankar Sabesan, Suresh V. Chinni
Summary: Biological macromolecules, such as polysaccharides, proteins, and glycoproteins, have been widely used in tissue engineering due to their ability to mimic the extracellular matrix and their biocompatibility. Scaffolds made from these macromolecules have properties like tensile strength, biodegradability, and flexibility. This review discusses the fabrication methods and applications of scaffolds using various biological macromolecules and emphasizes the importance of natural polymers over synthetic ones due to their unique properties.
Article
Biochemistry & Molecular Biology
Luong Huu Dang, Shih-Han Hung, Yuan Tseng, Ly Xuan Quang, Nhi Thao Ngoc Le, Chia-Lang Fang, How Tseng
Summary: Decellularization has been explored as a potential solution for tracheal replacement. Partial decellularization combined with autologous nasal epithelial cell sheets showed promising results in tracheal reconstruction, leading to integration with host tissue and adequate luminal surface healing two months post-operation. This hybrid scaffold protocol holds potential for future applications in tracheal reconstructions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Samantha Lo, Mh Busra Fauzi
Summary: Collagen, as a widely studied natural component, plays a major role in tissue repair and regeneration in tissue engineering. Nano collagen, a relatively new material involved in nanotechnology, has potential advantages over traditional collagen design due to its nano-size, making it suitable for various medical applications and cosmetic purposes.
Article
Ophthalmology
Yoshinori Oie, Seiichiro Sugita, Shunji Yokokura, Toru Nakazawa, Daisuke Tomida, Yoshiyuki Satake, Jun Shimazaki, Yuko Hara, Atsushi Shiraishi, Andrew J. Quantock, Takahiro Ogasawara, Masukazu Inoie, Kohji Nishida
Summary: This study confirmed the efficacy and safety of GMP-compliant autologous cultivated limbal epithelial cell sheets in treating patients with unilateral limbal stem cell deficiency. After 2 years of follow-up, corneal epithelial reconstruction was successful in 60% of eyes at 1 year and 70% of eyes at 2 years postoperatively. The improvement in visual acuity was also notable. Rating: 8/10.
Article
Engineering, Biomedical
Nastasia V. Kosheleva, Yuri M. Efremov, Polina I. Koteneva, Inna V. Ilina, Irina M. Zurina, Polina Y. Bikmulina, Anastasia I. Shpichka, Peter S. Timashev
Summary: Cell transitions between epithelial and mesenchymal phenotypes play a regulatory role in morphogenesis and regeneration. Tissue mechanics and mechanotransduction are essential in these processes. Cell spheroids provide a simple building block for tissue engineering purposes, and their mechanical properties have been studied using various techniques in this research.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Nazia Mehrban, Catalina Pineda Molina, Lina M. Quijano, James Bowen, Scott A. Johnson, Joseph Bartolacci, Jordan T. Chang, David A. Scott, Derek N. Woolfson, Martin A. Birchall, Stephen F. Badylak
ACTA BIOMATERIALIA
(2020)
Article
Engineering, Environmental
Ioan-Alexandru Baragau, Zhen Lu, Nicholas P. Power, David J. Morgan, James Bowen, Pedro Diaz, Suela Kellici
Summary: There is currently a lack of research and application of carbon-based nanomaterials for enhanced oil recovery. The use of sulfur-functionalized carbon quantum dots (S-CQDs) can significantly improve oil recovery rates in oil fields. S-CQDs exhibit excellent colloidal stability, low surface retention, and small particle size, which can effectively enhance oil recovery efficiency.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Physical
Zuzanna Trzcinska, Marc Bruggeman, Hanieh Ijakipour, Nikolas J. Hodges, James Bowen, Artemis Stamboulis
Article
Multidisciplinary Sciences
Sungwoo Lim, James Bowen, Giulia Degli-Alessandrini, Mahesh Anand, Aidan Cowley, Vibha Levin Prabhu
Summary: Our experiment found that higher microwave powers can generate materials with the highest yields and strongest microstructures in the shortest fabrication times. Thermal runaway improves the efficiency of microwave heating once triggered, which is crucial for future lunar ISRU demonstration missions.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Biomaterials
Nazia Mehrban, Daniela Cardinale, Santiago C. Gallo, Dani D. H. Lee, D. Arne Scott, Hanshan Dong, James Bowen, Derek N. Woolfson, Martin A. Birchall, Christopher O'Callaghan
Summary: Plasma treatment of polymers may promote the functional differentiation of airway epithelial cells. The synthetic peptide system described in this study offers potential superiority as an epithelial regeneration substrate over present gold-standard materials, such as collagen.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Dilveen Mohammed, Rayan Ameen, Steven Street, Kostas Sierros, James Bowen, Stephen Kukureka
Summary: Exposure to a corrosive NaCl solution can degrade the performance of ITO/Ag-alloy/ITO films over time, particularly at higher concentrations. The combination of stress and corrosion by the aqueous NaCl solution significantly reduces the conductivity of the film.
E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY
(2021)
Article
Engineering, Aerospace
Sungwoo Lim, Sam Reeve, Eneko Lekuona, Alberto Garbayo, Thomas Le Toux, Andrew Morse, James Bowen, Mahesh Anand
Summary: The sustainable exploration of the Moon could benefit from microwave heating-based 3D Printing technology, with the development of an MHD payload concept to perform ISRU experiments and generate valuable scientific data. Design criteria include sustainable heating performance at low microwave power levels, and considerations for phenomena during microwave heating of lunar regolith for future mission applications.
ADVANCES IN SPACE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Stephanie C. C. McClelland, Marie R. G. Attard, James Bowen, Nicholas P. C. Horrocks, Gabriel A. A. Jamie, Tanmay Dixit, Claire N. N. Spottiswoode, Steven J. J. Portugal
Summary: The eggs of avian brood-parasitic species have similar eggshell structures and compositions as their host's eggs, suggesting that the parasitic lifestyle does not greatly impact these traits.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Multidisciplinary Sciences
Marie R. G. Attard, James Bowen, Steven J. Portugal
Summary: Many bird species lay colored or patterned eggs, and the diversity of eggshell patterning is achieved through pigment. The study found that the foreground color of maculated eggs has a rougher surface compared to the background color. The texture of eggshells is correlated with various life-history traits.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Marie R. G. Attard, James Bowen, Rene Corado, Linnea S. Hall, Robert A. Dorey, Steven J. Portugal
Summary: Complex and extreme environments have led many bird species to develop unique eggshell surface properties to protect embryos from external threats; the wettability of eggshells is primarily explained by body mass, annual temperature, and eggshell maculation across nesting environments; distinct adaptations of eggshell wettability are identified in different nesting environments, driven by the need to retain heat and prevent microbial colonization.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)
Article
Chemistry, Multidisciplinary
Paheli Ghosh, Aruna Ivaturi, Debabrata Bhattacharya, James Bowen, Tony Nixon, Jan Kowal, Nicholas St John Braithwaite, Satheesh Krishnamurthy
MATERIALS TODAY CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Gabriela Melo Rodriguez, James Bowen, Mischa Zelzer, Artemis Stamboulis
Article
Chemistry, Physical
Avishek Dey, Paheli Ghosh, James Bowen, Nicholas St. J. Braithwaiteb, Satheesh Krishnamurthy
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2020)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)