Article
Critical Care Medicine
Duane J. Funk, Jared Bullard, Sylvan Lother, Gloria Vazquez Grande, Lauren Garnett, Kaylie Doan, Kerry Dust, Anand Kumar, Guillaume Poliquin, Jim Strong
Summary: This study aimed to determine the duration of infectiousness in ICU patients with COVID-19. By culturing nasopharyngeal samples, it was found that 8.3% of patients grew live virus within a median of 13 days post-initial positive test. The cycle threshold (Ct) of > 25 had the best ability to determine the absence of live virus.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Immunology
Joseph D. Lutgring, Farrell A. Tobolowsky, Kelly M. Hatfield, Nicholas B. Lehnertz, Maureen M. Sullivan, Karen G. Martin, Amelia Keaton, D. Joseph Sexton, Azaibi Tamin, Jennifer L. Harcourt, Natalie J. Thornburg, Sujan C. Reddy, John A. Jernigan
Summary: Research has shown that replication-competent virus is not detected in individuals with mild to moderate COVID-19 more than 10 days after symptom onset. It is unclear whether this applies to nursing home residents. None of the 273 specimens collected from nursing home residents >10 days from the initial positive test were culture positive.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Nanoscience & Nanotechnology
Lixian Jiang, Zhewei Li, Qingshun Dong, Xin Rong, Guifang Dong
Summary: In this study, high-stability 2D/3D perovskite thin films were successfully fabricated using TEAI and dual additives, NH4SCN and NH4Cl, for perovskite photodetectors. The optimized devices exhibited good performance and showed promise in light-intensity adders and optical communication systems.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Microbiology
G. Vidanapathirana, A. L. S. K. Angulmaduwa, T. S. Munasinghe, E. W. M. A. Ekanayake, P. Harasgama, S. T. Kudagammana, B. N. Dissanayake, L. V. C. Liyanapathirana
Summary: The colonization rate of pneumococci was higher when detected using RT-PCR compared to culture, although it was lower in healthy children. The pneumococcal colonization density in children with respiratory symptoms was higher, but not significantly different compared to previous studies. Further evaluation of RT-PCR for pneumococcal detection is needed.
Article
Medicine, Legal
S. Plenzig, F. Holz, D. Bojkova, M. Kettner, J. Cinatl, M. A. Verhoff, C. G. Birngruber, S. Ciesek, H. F. Rabenau
Summary: This study examined the detection of SARS-CoV-2 in corpses exhumed approximately 4 months after death, revealing that the virus could still be detected despite signs of decay. Results showed low infectivity risk for individuals involved in the exhumation, as testing of perioral and perinasal swabs came back negative. This demonstrates the stability of viral RNA in intact corpses even after months in a grave.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Construction & Building Technology
Jostein Gohli, Ane Marie Anderson, Arne Broch Brantsaeter, Kari Oline Boifot, Carola Grub, Cathrine Lund Hadley, Andreas Lind, Ellen Susanne Pettersen, Arne Vasli Lund Soraas, Marius Dybwad
Summary: In this study, the quantitative analysis of SARS-CoV-2 in the air surrounding infected individuals with mild symptoms was conducted. The results showed a significant correlation between viral concentration in the air samples collected at one-meter distance with viral loads in the upper airways, mild coughing, and fever. The findings have potential implications for SARS-CoV-2 detection, monitoring, and understanding of transmission dynamics.
Article
Microbiology
Hugo Alberto Barrera Saldana, Carolina Rivera Santiago, Raul Rodriguez Palacios
Summary: COVID-19 was first detected in Mexico in February 2020. Despite the initial lack of recognition by health authorities, we anticipated the need for viral detection tests and established a rapid molecular diagnostic service for SARS-CoV-2. Standardized protocols for biospecimen sampling, transportation, biobanking, preanalytical validation, and nucleic acids testing were implemented. The adoption of automation processes, a quality control/quality assurance system, and close collaboration with health authorities were key factors in our success.
MICROBIOLOGY SPECTRUM
(2022)
Article
Environmental Sciences
Guilherm Pereira Schoeler, Thays Franca Afonso, Carolina Faccio Demarco, Victor Dos Santos Barboza, Tito Roberto Sant'anna Cadaval Jr, Andrei Valerao Igansi, Marcos Alexandre Gelesky, Janice Luehring Giongo, Rodrigo de Almeida Vaucher, Rafael de Avila Delucis, Robson Andreazza
Summary: The COVID-19 pandemic has had unprecedented impacts on health and the economy, emphasizing the importance of basic sanitation and social equality. This study developed a low-cost and efficient polyurethane biofoam material for the removal of SARS-CoV-2 from water, showing promising results.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Orthopedics
Yasmin Badshah, Maria Shabbir, Hunza Hayat, Zoha Fatima, Asad Burki, Sidra Khan, Shafiq Ur Rehman
Summary: The study identified the association of IL-6, CALM1, and TGF-β1 gene polymorphisms with osteoarthritis in Pakistani patients. These genetic variations may serve as susceptible markers for OA and could be used for screening OA patients in Pakistan. Further research on larger populations is needed to confirm the role of these genetic factors in prediagnosis of OA.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2021)
Article
Immunology
Alessandra Luna-Muschi, Saidy Vasconez Noguera, Igor C. Borges, Anderson De Paula, Marina Farrel Cortes, Carolina Larocca, Julia Ferreira Mari, Lara Silva Pereira Guimaraes, Pablo Munoz Torres, Nazareno Scaccia, Lucy S. Villas-Boas, Almir Ribeiro da Silva Jr, Pamela S. Andrade, Juliana C. Teixeira, Camille Escadafal, Vitor Falcao de Oliveira, Tania R. Tozetto-Mendoza, Maria Cassia Mendes-Correa, Anna S. Levin, Ester C. Sabino, Silvia F. Costa
Summary: This study evaluated the duration of viral culture positivity compared to RAT and RT-PCR in mild Omicron infection, finding that RAT and RT-PCR can be useful predictors of viral culture positivity. Vaccinated individuals may still be transmissible up to day 7 post-infection, and lowering the RT-PCR threshold can improve culture prediction accuracy.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Food Science & Technology
Polett Hajdrik, Bernadett Palyi, Zoltan Kis, Noemi Kovacs, Daniel Sandor Veres, Krisztian Szigeti, Ferenc Budan, Imre Hegedus, Tibor Kovacs, Ralf Bergmann, Domokos Mathe
Summary: The study demonstrates that a combination of humic substances with ascorbic acid, Se, and Zn2+ ions has significant antiviral activity against the SARS-CoV-2 virus at very low concentrations.
Article
Infectious Diseases
Bo Hu, Zhiyu Fan, Rulong Qiu, Mengmeng Chen, Houjun Wei, Yanhua Song, Weilong Liu, Weizhong Xu, Fang Wang
Summary: This study reports a novel recombinant strain of rabbit hemorrhagic disease virus 2 (RHDV2 or GI.2) detected from domestic rabbits in China. The new strain showed differences in infected time and mortality rate compared to the original strain, but similar viral load at the moribund stage of infection. The presence and circulation of this novel variant should be carefully monitored in China.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Biophysics
Jinlei Wang, Weijun Xu, Junmin Qian, Yaping Wang, Guanghui Hou, Aili Suo
Summary: 2D cell culture fails to accurately replicate the features of real tumors, while 3D tumor models can better mimic the native tumor microenvironments. The degree of methacrylation has significant effects on the microstructure, mechanical properties, and degradation abilities of the hydrogel. The optimized hyaluronic acid hydrogel demonstrated favorable characteristics for facilitating cell proliferation and growth in a 3D culture environment.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Pharmacology & Pharmacy
Iosif Ilia, Doina Chioran, Daniela Radu, Raul Chioiba, Ioana Ionita, Catalin Marian, Alexandra Semenescu, Robert Cosmin Racea, Ramona Popovici, Stefania Dinu, Mircea Rivis, Andrada Iftode
Summary: Hyaluronic acid has the potential to be an effective candidate for the treatment of osteosarcoma, as it can induce cell apoptosis and alter gene expression. However, further research is needed to elucidate the molecular mechanisms involved.
Article
Gastroenterology & Hepatology
Huayu Yang, Lejia Sun, Yuan Pang, Dandan Hu, Haifeng Xu, Shuangshuang Mao, Wenbo Peng, Yanan Wang, Yiyao Xu, Yong-Chang Zheng, Shunda Du, Haitao Zhao, Tianyi Chi, Xin Lu, Xinting Sang, Shouxian Zhong, Xin Wang, Hongbing Zhang, Pengyu Huang, Wei Sun, Yilei Mao
Summary: The study focuses on 3D bioprinted hepatorganoids, liver tissue models generated using HepaRG cells, showing comprehensive liver functions in vitro and in vivo. After transplantation, the hepatorganoids improved the survival rate of mice, providing a potential alternative for treating liver failure.
Article
Engineering, Biomedical
Feihu Xie, Lejia Sun, Yuan Pang, Gang Xu, Bao Jin, Haifeng Xu, Xin Lu, Yiyao Xu, Shunda Du, Yanan Wang, Shi Feng, Xinting Sang, Shouxian Zhong, Xin Wang, Wei Sun, Haitao Zhao, Hongbing Zhang, Huayu Yang, Pengyu Huang, Yilei Mao
Summary: This study established a modeling system using three-dimensional bioprinting technology for patient-specific drug screening for hepatocellular carcinoma. The models retained features of parental HCCs, including stable biomarker expression and genetic alterations. Additionally, the models were capable of intuitively and quantitatively displaying drug screening results.
Article
Biochemistry & Molecular Biology
Yi Lu, Wentao Dai, Jianhua Huang, Xuexin Chen, Yuan Yao
Summary: This study revealed the biochemical composition and binding characteristics of the glue protein secreted by fruit fly larvae, identified chitin-binding motifs through bioinformatics and computational modeling, and synthesized a biomimetic glue protein with enhanced performance for cellular viability through a biosynthetic approach. These findings provide a potential biomacromolecule candidate for extracellular matrix in cell culture.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Cell & Tissue Engineering
Quanfu Xu, Pei Ying, Jing Ren, Na Kong, Yang Wang, Yi-Gang Li, Yuan Yao, David L. Kaplan, Shengjie Ling
Summary: This article discusses the importance of biomimetic design in rebuilding biofunctional interfaces to support adaptive human behavior, emphasizing the reconstruction of organ structure and physiological function to mimic nature. It also highlights the development and challenges of regenerative medicine, and the prerequisites for constructing multicellular organs.
TISSUE ENGINEERING PART B-REVIEWS
(2021)
Article
Cell Biology
Heming Wang, Rong Huang, Ling Li, Junjin Zhu, Zhihong Li, Chao Peng, Xuran Zhuang, Haifan Lin, Shuo Shi, Pengyu Huang
Summary: High-throughput sequencing was used to analyze the landscape of small noncoding RNAs, revealing a large number of previously undetected sRNAs. The study also reported extensive tissue-specific differences in sRNAs by comparing sRNomes from nine mouse tissues. Additionally, the researchers observed changes in sRNomes during hepatic reprogramming, and found that knockdown of a certain type of sRNA promoted this reprogramming process.
Article
Chemistry, Multidisciplinary
Yuehua Zhang, Shasha Ye, Leitao Cao, Zhuochen Lv, Jing Ren, Zhengzhong Shao, Yuan Yao, Shengjie Ling
Summary: This study develops a biomimetic meso-assembly processing engineering strategy to fabricate biomaterials with structural and mechanical characteristics mimicking biological tissues. The materials exhibit exceptional tunable mechanical properties and show potential in regulating cell morphology.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Letter
Cell Biology
Xiangyang Li, Xin Wang, Wenjun Sun, Shisheng Huang, Mingtian Zhong, Yuan Yao, Quanjiang Ji, Xingxu Huang
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Guiquan Zhang, Yao Liu, Shisheng Huang, Shiyuan Qu, Daolin Cheng, Yuan Yao, Quanjiang Ji, Xiaolong Wang, Xingxu Huang, Jianghuai Liu
Summary: This study developed an upgraded prime editor platform (xrPE) by adding a viral xrRNA motif to pegRNAs, resulting in significantly enhanced editing efficiencies in multiple cell lines. The xrPE exhibited similar edit:indel ratios and minimal off-target editing compared to the canonical PE3, and showed comparable editing performance to the epegRNA-based PE system.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Zhijie Li, Heming Wang, Junjin Zhu, Ning Nan, Yi Lin, Xuran Zhuang, Ling Li, Yamin Zhang, Pengyu Huang
Summary: The TWEAK/Tnfrsf12a axis activates RIPK1 in hepatocytes, leading to RIPK1-dependent apoptosis and liver injury in acute liver failure (ALF). Inhibition of either the TWEAK/Tnfrsf12a axis or RIPK1-dependent apoptosis attenuates liver injury, providing a new potential therapeutic target for the treatment of ALF.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Yangyang Yuan, Chenwei Wang, Xuran Zhuang, Shaofeng Lin, Miaomiao Luo, Wankun Deng, Jiaqi Zhou, Lihui Liu, Lina Mao, Wenbo Peng, Jian Chen, Qiangsong Wang, Yilai Shu, Yu Xue, Pengyu Huang
Summary: This study develops a method, central kinase inference, to predict key kinases by integrating quantitative transcriptomic and phosphoproteomic data. The method is more accurate than Kinase-Substrate Enrichment Analysis in predicting known kinases associated with anti-cancer drug resistance. The study also identifies key kinases involved in hepatocyte maturation and hepatic reprogramming.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Xiangyang Li, Guiquan Zhang, Shisheng Huang, Yao Liu, Jin Tang, Mingtian Zhong, Xin Wang, Wenjun Sun, Yuan Yao, Quanjiang Ji, Xiaolong Wang, Jianghuai Liu, Shiqiang Zhu, Xingxu Huang
Summary: Strategies to improve the specificity of nuclease-based prime editor (PEn) are needed. Here the authors report a 53BP1-inhibitory ubiquitin variant-assisted PEn platform (uPEn) to inhibit NHEJ and enable precise prime editing for generation of insertions, deletions, and replacements.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Guo Li, Xiaoning Zhu, Yihan Wang, Hongru Ma, Yuzhe Wang, Hanyu Wu, Xiangyang Li, Yiling Wang, Jianen Gao, Xuexin Chen, Xingxu Huang, Yuan Yao, Xiaoxiang Hu
Summary: RNA base editing is a promising tool for precise molecular therapy, and currently there are two widely used RNA base editors, REPAIR and RESCUE. REPAIR only facilitates A-to-I conversions, while RESCUE performs both A-to-I and C-to-U conversions, and can generate twice the number of mutations compared to REPAIR. However, the transcription-wide impact caused by RESCUE-induced off-target single-nucleotide variants (SNVs) is not fully understood. Therefore, this study used transcription-wide sequencing to determine the off-target effects of RESCUE-mediated editing and found different off-target effects on various RNA types.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Guo Li, Yaxian Cheng, Yeqiu Li, Hongru Ma, Zhongji Pu, Sa Li, Yiqiang Zhao, Xingxu Huang, Yuan Yao
Summary: We developed a new editor, SpRY-ABE8eF148A, which can mediate A-to-G and T-to-C transition mutations, suitable for repairing the most significant G & BULL;C-to-T & BULL;A pathogenic variants. Compared with SpRY-ABE8e, SpRY-ABE8eF148A narrows the editing range and enhances A-to-G editing efficiency in most sites with NR/YN PAMs. Furthermore, it significantly decreases the RNA off-target effect.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Editorial Material
Medicine, Research & Experimental
Chunlong Xu, Pengyu Huang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Yi Lu, Guo Li, Yeqiu Li, Yuan Yao
Summary: This study introduces an efficient method to drive rapid multicellular spheroid formation by a cellulose nanofibril matrix. The matrix enables the facilitated growth of spheroids through multiple cell assembly into size-controllable aggregates with well-organized physiological microstructure.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2023)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
