Article
Biotechnology & Applied Microbiology
Nikolett Miklovics, Agnes Duzs, Fanni Balogh, Gabor Paragi, Gabor Rakhely, Andras Toth
Summary: This study reports the characteristics of the quinone binding site (QBS) of a type VI sulfide:quinone oxidoreductases (SQR) from the photosynthetic purple sulfur bacterium Thiocapsa roseopersicina. The study identifies the amino acids near the enzyme-bound quinone ring and their interaction with the quinone head group. Mutant experiments further confirm the importance of these interactions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Lijie Zhou, Pingxiang Ou, Bikai Zhao, Wenyu Zhang, Ke Yu, Kang Xie, Wei-Qin Zhuang
Summary: Assimilatory and dissimilatory sulfate reduction (ASR and DSR) pathways play important roles in bacterial sulfate reduction during wastewater treatment. Metagenomic analysis revealed diverse microbial communities in biofoulants, with abundant functional genes related to ASR and DSR. ASR functional genes were more abundant in the biofoulants, but bacteria with complete DSR pathways were responsible for sulfide production in the biofilm-MBR.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Multidisciplinary
Marta Meneghello, Ana Rita Oliveira, Aurore Jacq-Bailly, Ines A. C. Pereira, Christophe Leger, Vincent Fourmond
Summary: Mo/W formate dehydrogenases catalyze the reversible reduction of CO2 to formate, and it has been definitively demonstrated through an electrochemical method that the substrate is indeed CO2, not a hydrated species like HCO3-.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Birgitte S. Jensen, Sibile Pardue, Brynne Duffy, Christopher G. Kevil, James F. Staples, Angela Fago
Summary: Hydrogen sulfide (H2S) plays a key role in regulating basal metabolic rate and mitochondrial respiration in hibernating mammals. During torpor, H2S accumulates in the liver and inhibits mitochondrial respiration, while during arousal and summer, this effect is reversed.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Laia Montoliu-Gaya, Daniel Tietze, Debora Kaminski, Ekaterina Mirgorodskaya, Alesia A. Tietze, Fredrik H. Sterky
Summary: Neurexins are presynaptic adhesion molecules that regulate the molecular composition of synapses by diversifying in numerous isoforms. The study reveals that CA10 in the secretory pathway regulates neurexin-HS formation, blocking HS biosynthesis through a direct interaction and suggesting a new mechanism to regulate glycosylations. These findings provide insights into the regulation of neurexins and demonstrate a novel approach to modulate site-specific glycosylations.
Article
Chemistry, Medicinal
Gustavo Provensi, Alessia Costa, Barbara Rani, Maria Vittoria Becagli, Fabio Vaiano, Maria Beatrice Passani, Damiano Tanini, Antonella Capperucci, Simone Carradori, Jacobus P. Petzer, Anel Petzer, Daniela Vullo, Gabriele Costantino, Patrizio Blandina, Andrea Angeli, Claudiu T. Supuran
Summary: This study reported the synthesis of β-arylchalcogeno amines with structural similarity to amphetamine, which showed good activation properties for certain enzymes in the brain. In vivo evaluation demonstrated the procognitive effects of these compounds without causing unwanted side effects. These findings suggest the potential utility of these compounds for improving cognitive decline associated with neurodegenerative and psychiatric diseases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Ozlem Akgul, Srishti Singh, Jacob T. Andring, Robert McKenna, Silvia Selleri, Fabrizio Carta, Andrea Angeli, Claudiu T. Supuran
Summary: A series of taurine substituted sulfonamide derivatives with ureido moiety at the tail section were synthesized as selective inhibitors of tumor associated human Carbonic Anhydrase (CA) IX and XII. These derivatives showed a strong dependence on the presence of ureido moiety and demonstrated highly stabilized ligand-protein complex with specific amino acid residues in both hCA II and hCA IX-mimic.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Chemistry, Medicinal
Shubham Kumar, Sandeep Rulhania, Shalini Jaswal, Vikramdeep Monga
Summary: Carbonic anhydrase is an important enzyme involved in various physiological and pathological processes, with 16 different isoforms in humans. Inhibitors targeting different isoforms of carbonic anhydrase have clinical applications in treating various diseases, but current drugs lack selectivity leading to undesired side effects. Efforts are being made to develop novel isoform-selective inhibitors of carbonic anhydrase for therapeutic implications.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Xiaojing Huang, Daniel Winter, Dominic. J. J. Glover, Claudiu. T. T. Supuran, William. A. A. Donald
Summary: Carbonic anhydrases (CAs) are metalloenzymes that play important roles in cellular processes and have been implicated in various diseases. Phosphorylation, a common post-translational modification of CAs, can significantly impact their catalytic activity and drug-binding capabilities. This study highlights the potential regulatory role of phosphorylation in CA activity and its effect on small molecule drug binding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Mohammed Al-Zubaidi, Pravin Viswambaram, Steve McCombie, Elizabeth Liow, Nat Lenzo, Tom Ferguson, Andrew D. Redfern, Richard Gauci, Dickon Hayne
Summary: Bladder cancer is a lethal disease with limited conventional imaging modalities for accurate staging. This study aims to investigate the use of (89)Zirconium-labelled girentuximab PET in the staging of bladder and urothelial carcinomas.
Review
Chemistry, Multidisciplinary
Cristhian Molina-Fernandez, Patricia Luis
Summary: The use of immobilized carbonic anhydrase as a novel approach for CO2 capture presents a promising alternative to address cost and environmental issues. Current research focuses on developing different carriers and immobilization strategies to achieve stability and activity compared to free enzyme in solution.
JOURNAL OF CO2 UTILIZATION
(2021)
Article
Biochemistry & Molecular Biology
Amarjyoti Das Mahapatra, Aarfa Queen, Mohd Yousuf, Parvez Khan, Afzal Hussain, Md. Tabish Rehman, Mohamed F. Alajmi, Bhaskar Datta, Md. Imtaiyaz Hassan
Summary: Inhibitors of carbonic anhydrase have the potential to address various diseases. This research focuses on developing a new scaffold, oxazole-5(4H)-one derivatives, for the selective inhibition of human carbonic anhydrase VA (hCAVA). Two compounds show high binding affinity and inhibit the expression of CAVA and GLUT4 in adipocytes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Environmental Sciences
Yan Sun, Minxiao Wang, Zhaoshan Zhong, Hao Chen, Hao Wang, Li Zhou, Lei Cao, Lulu Fu, Huan Zhang, Chao Lian, Song Sun, Chaolun Li
Summary: This study investigated the adaptive strategies and sulfide detoxification mechanisms of the deep-sea mussel Gigantidas plantifrons in hydrothermal vent and methane seep environments. The results revealed active sulfide detoxification in the gills of G. platifrons, with epibiotic Campylobacterota bacteria and endosymbiotic microbes playing key roles in the oxidation of extracellular and intracellular sulfide respectively. Transcriptional analysis showed the collaboration between epibionts, endosymbionts, and host mussels in sulfide detoxification to adapt to harsh H2S-rich environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Vaha Akbary Moghaddam, Vesal Kasmaeifar, Zainab Mahmoodi, Hossein Ghafouri, Omid Saberi, Asadollah Mohammadi
Summary: A novel derivative of sulfamethoxazole named ZM-093 was synthesized and characterized through experimental and computational analysis, showing significant inhibitory effect against the cancer-related protein HSP70, potentially through binding to its substrate-binding domain. Additionally, a new method for evaluating the inhibitory activity of ligands to HSP70 based on protonography was introduced.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Xianqin Song, Yu Song, Quanping Ma, Kehua Fang, Xiaotian Chang
Summary: This study found that M1 macrophages can increase the expression of CA1 and CA2 to promote atherosclerotic calcification in vascular smooth muscle cells (VSMCs) by secreting TNF-a.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Microbiology
Shih-Yao Lin, Wen-Ming Chen, Asif Hameed, Guan-Hua Huang, Mei-Hua Hung, Chiu-Chung Young
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)
Article
Microbiology
Shih-Yao Lin, Chia-Fang Tsai, Asif Hameed, Chiu-Chung Young
Summary: In this study, a polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium isolated from soil in a maize field in Taiwan, which was named Aeromicrobium terrae. Analyses based on phylogeny, phenotypic traits, chemotaxonomic characteristics, 16S rRNA gene sequences, and ANI values supported the proposal of this bacterium as a novel species within the genus Aeromicrobium.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Shih-Yao Lin, Chia-Fang Tsai, Asif Hameed, Yu-Shan Tang, Chiu- Chung Young
Summary: A polyphasic taxonomic approach was used to characterize a novel species, Devosia faecipullorum sp. nov., isolated from poultry manure in Taiwan. The strain CC-YST696T showed distinct phylogenetic, phenotypic and chemotaxonomic traits compared to other related species. This study provides valuable insights into the microbial diversity in poultry manure and highlights the importance of studying antibiotic and toxic compound resistance in environmental bacteria.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Shih-Yao Lin, Asif Hameed, Chia-Fang Tsai, Chiu-Chung Young
Summary: A Gram-stain-negative bacterium named Vineibacter terrae gen. nov., sp. nov. was isolated from vineyard soil in Taiwan. This bacterium is aerobic, non-motile, and capable of reducing nitrate, with optimal growth conditions at 30 degrees C and pH 7, belonging to the family Rhodospirillaceae.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Shih-Yao Lin, Asif Hameed, Chia-Fang Tsai, Yu-Shan Tang, Chiu-Chung Young
Summary: This study characterized a novel bacterium isolated from poultry manure and named it Pusillimonas faecipullorum sp. nov. It showed distinct phylogenetic, phenotypic, and chemotaxonomic traits, making it a new species in the genus Pusillimonas.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Shih-Yao Lin, Asif Hameed, Chia-Fang Tsai, Mei-Hua Hung, Chiu-Chung Young
Summary: A polyphasic taxonomic approach was used to characterize a novel species of the genus Agrilactobacillus, designated Agrilactobacillus fermenti sp. nov. Strain CC-MHH1034(T) was isolated from a fermented vegetable residue and exhibited distinctive phenotypic and chemotaxonomic traits. The results of comparative 16S rRNA gene sequence, OrthoANI, dDDH, and phylogenomic placement supported the establishment of this novel species within the family Lactobacillaceae.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Shih-Yao Lin, Chia -Fang Tsai, Asif Hameed, Tzung-Han Lee, Chiu- Chung Young
Summary: A polyphasic taxonomic approach was used to characterize a strain CC-SYL272(T) isolated from paddy soil, which was identified as a new species of the genus Niabella and named Niabella agricola sp. nov. This study provides important insights into the classification of the genus Niabella.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Soil Science
Asif Hameed, Yu -Pei Chen, Fo-Ting Shen, Shih-Yao Lin, Hsin- Huang, Yu -Wen Lin, Chiu-Chung Young
Summary: This study assessed the impacts of long-term fertilizer inputs on grain yield and edaphic factors of upland maize and wetland rice rotation system. The results showed that maize yield significantly increased under chemical fertilizer (CF), green manure (GMN1), and swine manure compost (CP)-based inputs. However, rice yield displayed marginal improvement or negative results. Principal component analysis revealed distinct clustering of soil factors during maize-rice rotation. CP-based inputs improved organic matter, electrical conductivity, microbial metabolism, and nutrient availability. Soil microbial activity and bacterial abundances were higher during maize cultivation compared to rice cultivation. In conclusion, long-term CP-based inputs significantly increased maize yield, but also acidified the soil.
APPLIED SOIL ECOLOGY
(2023)
Article
Microbiology
Kanadan Anjali, Muhammed Manzoor, Mangesh Vasant Suryavanshi, Parthiban Rudrapathy, Punchappady Devasya Rekha, Ranajit Das, Asif Hameed, Ananthapadmanabha Bhagwath Arun
Summary: Radiotherapy has an influence on the diversity and composition of the oral microbiome in patients with oral squamous cell carcinoma (OSCC). OSCC patients have a reduced species richness and altered microbial composition compared to controls. Significant shifts in the oral microbiome composition occur after six months of radiation therapy, including increased beneficial taxa and reduced pathogenic bacteria.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Microbiology
Shih-Yao Lin, Asif Hameed, Chia-Fang Tsai, Yu-Shan Tang, Chiu-Chung Young
Summary: A novel bacterium, designated strain CC-YST710(T), was characterized from poultry manure sampled in Taiwan using a polyphasic taxonomic approach, revealing it to represent a new Pseudogemmobacter species.
FEMS MICROBIOLOGY LETTERS
(2022)