Article
Infectious Diseases
Anna-Clara Ivarsson, Elin Fransen, Ioanna Broumou, Anna Farnert, Kristina E. M. Persson, Sara Karlsson Sobirk
Summary: This study compared the diagnostic performance of two LAMP kits for malaria detection and found that the HumaTurb Loopamp Malaria PDT kit outperformed the Alethia Illumigene Malaria kit.
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Multidisciplinary Sciences
Anita Rauzana, Wira Dharma
Summary: This study aims to identify the risk factors causing delays in construction projects in Aceh Province. The data analysis revealed 60 delay factors, with 30 falling into the category of very influential, 29 in the high influence category, and one in the medium influence category.
Article
Pathology
Eric McGinnis, Geoffrey Chan, Monika Hudoba, Todd Markin, Jim Yakimec, Kristine Roland
Summary: After implementing LAMP as a frontline screening test for malaria, the study successfully reduced reliance on microscopic evaluations and RDT in a multicenter health region, providing significant labor time savings without compromising turnaround times.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2021)
Review
Pathology
Oloche Owoicho, Charles Ochieng' Olwal, Edward Jenner Tettevi, Bernard Ortwer Atu, Ernest Uzodimma Durugbo
Summary: Non-albicans Candida species (NACS) are underestimated in terms of their public health burden, and outbreaks of candida infections require constant surveillance. Loop-mediated isothermal amplification (LAMP) is an affordable technique that can be used for Candida species surveillance.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2022)
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Medicine, Legal
Seiji Kubo, Keito Amai, Jin Tanaka, Hideki Niimi
Summary: This study reports a one-tube, two-step isothermal amplification assay for rapid and sensitive screening of saliva samples. The assay can be performed in a closed tube and the products are detected using real-time fluorescence analysis. The strategy shows promise for screening saliva from forensic samples.
FORENSIC SCIENCE INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Review
Medicine, General & Internal
Ulrika Morris, Berit Aydin-Schmidt
Summary: Loop-mediated isothermal amplification (LAMP) is a sensitive molecular tool suitable for malaria diagnosis, with high sensitivity and specificity in various settings. Despite limited commercial use, research has focused on specific applications such as malaria endemic areas, pregnancy screening, and returning travelers in non-endemic settings.
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Infectious Diseases
Cristina Costales, Mora Jana Broadhurst, Indre Budvytiene, Niaz Banaei
Summary: The Alethia Malaria LAMP assay showed high sensitivity and specificity in malaria screening and can be used as a stand-alone screening method. This method has advantages in non-endemic areas.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Herdiana Herdiana, Irnawati Irnawati, Farah Novita Coutrier, Alfian Munthe, Mardiati Mardiati, Titik Yuniarti, Elvieda Sariwati, Maria Endang Sumiwi, Rintis Noviyanti, Paul Pronyk, William A. Hawley
Article
Multidisciplinary Sciences
Zuleima Pava, Rintis Noviyanti, Irene Handayuni, Hidayat Trimarsanto, Leily Trianty, Faustina H. Burdam, Enny Kenangalem, Retno A. S. Utami, Yusrifar K. Tirta, Farah Coutrier, Jeanne R. Poespoprodjo, Ric N. Price, Jutta Marfurt, Sarah Auburn
Review
Multidisciplinary Sciences
Herdiana Herdiana, Jana Fitria Kartika Sari, Maxine Whittaker
Article
Infectious Diseases
Farah N. Coutrier, Yusrifar K. Tirta, Chris Cotter, Iska Zarlinda, Iveth J. Gonzalez, Alanna Schwartz, Cut Maneh, Jutta Marfurt, Maxwell Murphy, Herdiana Herdiana, Nicholas M. Anstey, Bryan Greenhouse, Michelle S. Hsiang, Rintis Noviyanti
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Infectious Diseases
Rintis Noviyanti, Olivo Miotto, Alyssa Barry, Jutta Marfurt, Sasha Siegel, Nguyen Thuy-Nhien, Huynh Hong Quang, Nancy Dian Anggraeni, Ferdinand Laihad, Yaobao Liu, Maria Endang Sumiwi, Hidayat Trimarsanto, Farah Coutrier, Nadia Fadila, Najia Ghanchi, Fatema Tuj Johora, Agatha Mia Puspitasari, Livingstone Tavul, Leily Trianty, Retno Ayu Setya Utami, Duoquan Wang, Kesang Wangchuck, Ric N. Price, Sarah Auburn
Article
Infectious Diseases
Lenny L. Ekawati, Kelly C. Johnson, Jerry O. Jacobson, Carmen A. Cueto, Iska Zarlinda, Iqbal R. F. Elyazar, Abdul Fatah, Maria E. Sumiwi, Rintis Noviyanti, Chris Cotter, Jennifer L. Smith, Farah N. Coutrier, Adam Bennett
Review
Public, Environmental & Occupational Health
Daniel Maxwell, Kelly C. Sanders, Oliver Sabot, Ahmad Hachem, Alejandro Llanos-Cuentas, Ally Olotu, Roly Gosling, James B. Cutrell, Michelle S. Hsiang
Summary: This study reviewed the current investigation of oral or potentially oral COVID-19 therapeutic agents in three different clinical trial registries, and summarized the number of trials and results of these agents. The findings indicate limited investment in COVID-19 therapeutics compared to vaccines, with a low proportion of completed trials and mostly negative results.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Medicine, Research & Experimental
Lisa J. Ioannidis, Halina M. Pietrzak, Ann Ly, Retno As Utami, Emily M. Eriksson, Stephanie Studniberg, Waruni Abeysekera, Connie S. N. Li-Wai-Suen, Dylan Sheerin, Julie Healer, Agatha M. Puspitasari, Dwi Apriyanti, Farah N. Coutrier, Jeanne R. Poespoprodjo, Enny Kenangalem, Benediktus Andries, Pak Prayoga, Novita Sariyanti, Gordon K. Smyth, Leily Trianty, Alan F. Cowman, Ric N. Price, Rintis Noviyanti, Diana S. Hansen
Summary: IFN-gamma-driven responses to malaria modulate the development and function of T follicular helper cells and memory B cells, with distinct populations associated with either increased or reduced risk of Plasmodium vivax malaria. This suggests that inflammatory responses to malaria are not universally harmful for infection.
Article
Biochemistry & Molecular Biology
Stephanie Studniberg, Lisa J. Ioannidis, Retno A. S. Utami, Leily Trianty, Yang Liao, Waruni Abeysekera, Connie S. N. Li-Wai-Suen, Halina M. Pietrzak, Julie Healer, Agatha M. Puspitasari, Dwi Apriyanti, Farah Coutrier, Jeanne R. Poespoprodjo, Enny Kenangalem, Benediktus Andries, Pak Prayoga, Novita Sariyanti, Gordon K. Smyth, Alan F. Cowman, Ric N. Price, Rintis Noviyanti, Wei Shi, Alexandra L. Garnham, Diana S. Hansen
Summary: This study investigates the differences between individuals experiencing symptomatic and asymptomatic P. falciparum infection using a systems approach. The results show that asymptomatic malaria, despite having protective responses, still exhibits an immunosuppressive transcriptional signature, which may impact the control of parasites and the response to malaria vaccines.
MOLECULAR SYSTEMS BIOLOGY
(2022)
Article
Infectious Diseases
Rintis Noviyanti, Kelly Carey-Ewend, Leily Trianty, Christian Parobek, Agatha Mia Puspitasari, Sujata Balasubramanian, Zackary Park, Nicholas Hathaway, Retno A. S. Utami, Saraswati Soebianto, Jeny Jeny, Frilasita Yudhaputri, Aditya Perkasa, Farah N. Coutrier, Yusrifar K. Tirta, Lenny Ekawati, Bagus Tjahyono, Inge Sutanto, Erni J. Nelwan, Herawati Sudoyo, J. Kevin Baird, Jessica T. Lin
Summary: Genotyping Plasmodium vivax relapses provides insights into the biology of hypnozoite. A study on Indonesian soldiers showed that the majority of relapses were driven by a single hypnozoite clone, even though most infected individuals harbored multiple lineages of hypnozoites. The number of detected parasite clones decreased over time, suggesting independent activation and slow depletion of hypnozoites over many months.
PLOS NEGLECTED TROPICAL DISEASES
(2022)