Article
Immunology
Wei Zhou, Sheng Peng, Peng Du, Ping Zhou, Chengbiao Xue, Qifa Ye
Summary: Ischemia-reperfusion injury (IRI) is a serious problem in DCD liver transplantation. Hypothermic oxygenated perfusion (HOPE) and the MyD88 inhibitor TJ-5 have been found to reduce oxidative stress, inflammation, and apoptosis in DCD liver IRI. This study demonstrates that combining HOPE with TJ-5 effectively alleviates DCD liver IRI by inhibiting the TLR/MyD88 signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Surgery
Damiano Patrono, Marinella Zanierato, Marco Vergano, Chiara Magaton, Enrico Diale, Giorgia Rizza, Silvia Catalano, Stefano Mirabella, Donatella Cocchis, Raffaele Potenza, Sergio Livigni, Roberto Balagna, Renato Romagnoli
Summary: The combination of A-NRP and D-HOPE in DCD liver transplantation allows for comparable outcomes to DBD transplantation.
TRANSPLANT INTERNATIONAL
(2022)
Article
Cardiac & Cardiovascular Systems
Niels Moeslund, Imran A. Ertugrul, Michiel A. Hu, Frederik Flyvholm Dalsgaard, Lars Bo Ilkjaer, Pia Ryhammer, Michael Pedersen, Michiel E. Erasmus, Hans Eiskjaer
Summary: In this study, a novel oxygenated hypothermic machine perfusion (HMP) system was compared to static cold storage (SCS) in heart transplantation (HTX). The results showed that hearts preserved with HMP exhibited increased biventricular contractility post-HTX and required less dobutamine for maintaining cardiac output. Additionally, HMP groups had lower levels of myocardial damage. Therefore, DCD HTX can be successfully performed using DPP followed by preservation with HMP.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Medicine, General & Internal
Rebecca Panconesi, Mauricio Flores Carvalho, Janina Eden, Marilena Fazi, Fariha Ansari, Leandro Mancina, Nadia Navari, Richard Xavier Sousa Da Silva, Daniele Dondossola, Lucia Bautista Borrego, Matthias Pietzke, Adriano Peris, David Meierhofer, Paolo Muiesan, Alexander Galkin, Fabio Marra, Philipp Dutkowski, Andrea Schlegel
Summary: This study investigated the effects of NRP and HOPE on DCD liver transplantation using an animal model. The results showed that HOPE can reduce mitochondrial injury and inflammation, and improve post-transplant survival.
Article
Health Care Sciences & Services
Federica Rigo, Nicola De Stefano, Damiano Patrono, Victor De Donato, Ludovico Campi, Diana Turturica, Teresa Doria, Veronica Sciannameo, Paola Berchialla, Francesco Tandoi, Renato Romagnoli
Summary: The study aimed to investigate the impact of dual-hypothermic oxygenated machine perfusion (D-HOPE) on hepatocellular carcinoma (HCC) recurrence after liver transplantation (LT). The results showed that D-HOPE did not reduce HCC recurrence but allowed the use of extended criteria donor livers, with comparable postoperative outcomes to static cold storage (SCS).
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Surgery
Rahel K. Wyss, Natalia Mendez Carmona, Maria Arnold, Adrian Segiser, Matteo Mueller, Philipp Dutkowski, Thierry P. Carrel, Sarah L. Longnus
Summary: The study investigated a new method for heart transplants used in DCD, called HOPE, which provides improved cardioprotection by reducing cardiac succinate levels, alleviating graft injury.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Medicine, General & Internal
Janina Eden, Eva Breuer, Dominique Birrer, Matteo Mueller, Matthias Pfister, Hemma Mayr, Keyue Sun, Jeannette Widmer, Florian Huwyler, Udo Ungethuem, Bostjan Humar, Anurag Gupta, Stefanie Schiess, Martin Wendt, Franz Immer, Andreas Elmer, David Meierhofer, Andrea Schlegel, Philipp Dutkowski
Summary: This study reports on the concept of liver assessment during ex situ hypothermic oxygenated perfusion (HOPE) and emphasizes its significant impact on liver utilization. The findings show that HOPE treatment combined with viability assessment is highly suitable for evaluating liver grafts, enabling surgeons to make informed decisions on whether to proceed with the transplant.
Article
Medicine, General & Internal
Sodai Sakamoto, Hiroki Bochimoto, Kengo Shibata, Nur Khatijah Mohd Zin, Moto Fukai, Kosei Nakamura, Takahisa Ishikawa, Masato Fujiyoshi, Tsuyoshi Shimamura, Akinobu Taketomi
Summary: This study aimed to verify the optimal pH of hypothermic machine perfusion (HMP) for donation after circulatory death (DCD) livers. The results showed that the HMP group with pH 7.8 displayed significant graft protection, as evidenced by lower enzyme levels, bile production, diminished tissue injury, and well-preserved mitochondrial structure. Therefore, HMP with a pH of 7.8 enhances protective effects by preserving mitochondrial structure and function, reducing reperfusion injury in DCD livers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Immunology
Riccardo De Carlis, Andrea Schlegel, Samuele Frassoni, Tiziana Olivieri, Matteo Ravaioli, Stefania Camagni, Damiano Patrono, Domenico Bassi, Duilio Pagano, Stefano Di Sandro, Andrea Lauterio, Vincenzo Bagnardi, Salvatore Gruttadauria, Umberto Cillo, Renato Romagnoli, Michele Colledan, Matteo Cescon, Fabrizio Di Benedetto, Paolo Muiesan, Luciano De Carlis
Summary: This study found that NRP and D-HOPE yield good results in DCD livers with prolonged warm ischemia in Italy, reducing the incidence of kidney injury.
Article
Cell Biology
Wei Zhou, Zibiao Zhong, Danni Lin, Zhongzhong Liu, Qiuyan Zhang, Haoyang Xia, Sheng Peng, Anxiong Liu, Zhongshan Lu, Yanfeng Wang, Shaojun Ye, Qifa Ye
Summary: Ischemia-reperfusion injury (IRI) is a serious issue in donations after heart death (DCD) liver transplantation, and hypothermic oxygenated perfusion (HOPE) has been shown to reduce liver IRI by regulating inflammation. HOPE can protect against DCD liver transplantation-induced-liver IRI by reducing inflammation via HECTD3-mediated TRAF3 K63-linked polyubiquitination.
CELL DEATH & DISEASE
(2021)
Review
Immunology
Laura Anguela-Calvet, Gabriel Moreno-Gonzalez, Fabrizio Sbraga, Jose Gonzalez-Costello, Steven Tsui, Eva Oliver-Juan
Summary: Cardiac transplantation is the gold-standard therapy for advanced-stage heart failure, with the option of DCD donation increasing the donor pool. Programs utilizing cDCD for heart transplants have been implemented in several countries, with ongoing improvements in techniques and strategies, emphasizing the need for implementation in experienced cardiac transplant centers.
Article
Surgery
Otto B. van Leeuwen, Silke B. Bodewes, Veerle A. Lantinga, Martijn P. D. Haring, Adam M. Thorne, Isabel M. A. Bruggenwirth, Aad P. van den Berg, Marieke T. de Boer, Iris E. M. de Jong, Ruben H. J. de Kleine, Bianca Lascaris, Maarten W. N. Nijsten, Koen M. E. M. Reyntjens, Vincent E. de Meijer, Robert J. Porte
Summary: Ex situ machine perfusion using sequential DHOPE-NMP for resuscitation and viability assessment of high-risk donor livers results in excellent transplant outcomes, irrespective of the oxygen carrier used.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Medicine, General & Internal
Fabian Horne, Moritz Drefs, Malte Joachim Schirren, Dominik Thomas Koch, Ganildo Cepele, Severin Johannes Jacobi, Elnaz Payani, Nikolaus Boerner, Jens Werner, Markus Otto Guba, Dionysios Koliogiannis
Summary: The study demonstrates that pre-transplant graft preconditioning with HOPE can reduce the incidence of PRS and EAD in liver transplantation, as well as improve hemodynamic stability. HOPE can also mitigate ES and prevent hyperpotassemic complications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Gastroenterology & Hepatology
Andrea Schlegel, Robert Porte, Philipp Dutkowski
Summary: The development of cholangiopathies after liver transplantation impacts on the quality and duration of graft and patient survival. The clinical implementation of dynamic preservation strategies, such as hypothermic oxygenated perfusion (HOPE), has shown promising results in reducing biliary complications.
JOURNAL OF HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Janina Eden, Richard Xavier Sousa Da Silva, Miriam Cortes-Cerisuelo, Kristopher Croome, Riccardo De Carlis, Amelia J. Hessheimer, Xavier Muller, Femke de Goeij, Vanessa Banz, Giulia Magini, Philippe Compagnon, Andreas Elmer, Andrea Lauterio, Rebecca Panconesi, Jeannette Widmer, Daniele Dondossola, Paolo Muiesan, Diethard Monbaliu, Marieke de Rosner van van Rosmalen, Olivier Detry, Constantino Fondevila, Ina Jochmans, Jacques Pirenne, Franz Immer, Gabriel C. Oniscu, Jeroen de Jonge, Mickael Lesurtel, Luciano G. De Carlis, C. Burcin Taner, Nigel Heaton, Andrea Schlegel, Philipp Dutkowski
Summary: Liver graft utilization rates and discard rates vary across countries, with machine perfusion strategies contributing to better DCD utilization rates. Risk factors and use of various machine perfusion techniques vary between countries, but this does not significantly impact 5-year graft survival rates. Overall, countries with routine use of machine perfusion strategies have higher DCD utilization rates without compromised outcomes.
JOURNAL OF HEPATOLOGY
(2023)
Article
Surgery
Marjolein van Reeven, Otto B. van Leeuwen, Danny van der Helm, Sarwa Darwish Murad, Aad P. van den Berg, Bart van Hoek, Ian P. J. Alwayn, Wojciech G. Polak, Robert J. Porte
TRANSPLANT INTERNATIONAL
(2020)
Article
Surgery
Otto B. van Leeuwen, Marjolein van Reeven, Danny van der Helm, Jan N. M. IJzermans, Vincent E. de Meijer, Aad P. van den Berg, Sarwa Darwish Murad, Bart van Hoek, Ian P. J. Alwayn, Robert J. Porte, Wojciech G. Polak
Letter
Gastroenterology & Hepatology
Otto B. van Leeuwen, Isabel M. A. Bruggenwirth, Robert J. Porte, Paulo N. Martins
Article
Surgery
Marjolein van Reeven, Nicholas Gilbo, Diethard Monbaliu, Otto B. van Leeuwen, Robert J. Porte, Dirk Ysebaert, Bart van Hoek, Ian P. J. Alwayn, Nicolas Meurisse, Olivier Detry, Laurent Coubeau, Olga Cicarelli, Frederik Berrevoet, Aude Vanlander, Jan N. M. IJzermans, Wojciech G. Polak
Review
Biochemistry & Molecular Biology
Silke B. Bodewes, Otto B. van Leeuwen, Adam M. Thorne, Bianca Lascaris, Rinse Ubbink, Ton Lisman, Diethard Monbaliu, Vincent E. De Meijer, Maarten W. N. Nijsten, Robert J. Porte
Summary: Oxygenated ex situ machine perfusion of donor livers is an alternative method for static cold preservation. Oxygen delivery is crucial for preserving organ viability, although challenging. Different oxygen carriers have their own advantages and disadvantages in organ preservation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
R. Van Rijn, I. J. Schurink, Y. de Vries, A. P. van den Berg, M. Cortes Cerisuelo, S. Darwish Murad, J. I. Erdmann, N. Gilbo, R. J. de Haas, N. Heaton, B. van Hoek, V. A. L. Huurman, I. Jochmans, O. B. van Leeuwen, V. E. de Meijer, D. Monbaliu, W. G. Polak, J. J. G. Slangen, R. I. Troisi, A. Vanlander, J. de Jonge, R. J. Porte
Summary: This study demonstrated that hypothermic oxygenated machine perfusion can reduce the risk of nonanastomotic biliary strictures following liver transplantation from donors after circulatory death, with lower rates of complications compared to conventional static cold storage.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Letter
Multidisciplinary Sciences
Otto B. van Leeuwen, Yvonne de Vries, Vincent E. de Meijer, Robert J. Porte
NATURE COMMUNICATIONS
(2021)
Review
Gastroenterology & Hepatology
Isabel M. A. Bruggenwirth, Otto B. van Leeuwen, Robert J. Porte, Paulo N. Martins
Summary: The transplant community is facing challenges due to the shortage of suitable liver donor organs and the increasing number of unused livers with deteriorating quality. Ex situ machine perfusion provides additional data for assessing liver graft viability and predicting postoperative risks.
LIVER TRANSPLANTATION
(2022)
Article
Transplantation
Yvonne de Vries, Isabel M. A. Bruggenwirth, Shanice A. Karangwa, Fien A. von Meijenfeldt, Otto B. van Leeuwen, Laura C. Burlage, Iris E. M. de Jong, Annette S. H. Gouw, Vincent E. de Meijer, Ton Lisman, Robert J. Porte
Summary: This study suggests that there are no significant differences in hepatobiliary or endothelial function and injury after preservation by single or dual HOPE of porcine livers donated after circulatory death.
TRANSPLANTATION DIRECT
(2021)
Article
Engineering, Biomedical
Isabel M. A. Bruggenwirth, Willemijn S. van der Plas, Otto B. van Leeuwen, Adam M. Thorne, Michel Rayar, Vincent E. de Meijer, Robert J. Porte
Summary: Oxygenated flush and storage of DCD porcine livers can prevent ATP depletion during ischemia, but may not be sufficient to mitigate early signs of IRI.
Article
Surgery
Otto B. van Leeuwen, Silke B. Bodewes, Veerle A. Lantinga, Martijn P. D. Haring, Adam M. Thorne, Isabel M. A. Bruggenwirth, Aad P. van den Berg, Marieke T. de Boer, Iris E. M. de Jong, Ruben H. J. de Kleine, Bianca Lascaris, Maarten W. N. Nijsten, Koen M. E. M. Reyntjens, Vincent E. de Meijer, Robert J. Porte
Summary: Ex situ machine perfusion using sequential DHOPE-NMP for resuscitation and viability assessment of high-risk donor livers results in excellent transplant outcomes, irrespective of the oxygen carrier used.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Surgery
Veerle A. Lantinga, Carlijn Buis, Robert J. Porte, Vincent E. de Meijer, Otto B. van Leeuwen
Summary: This study demonstrated that cold ischemia time in liver transplantation can be effectively reduced by using portal vein only HOPE during the back-table preparation.
CLINICAL TRANSPLANTATION
(2022)
Letter
Gastroenterology & Hepatology
Isabel M. A. Bruggenwirth, Otto B. van Leeuwen, Matteo Mueller, Philipp Dutkowski, Diethard Monbaliu, Paulo N. Martins, Robert J. Porte, Vincent E. de Meijer
Article
Engineering, Biomedical
Isabel M. A. Bruggenwirth, Willemijn S. van der Plas, Otto B. van Leeuwen, Adam M. Thorne, Michel Rayar, Vincent E. de Meijer, Robert J. Porte
Summary: The study found that oxygenated flush out and storage of DCD porcine livers can prevent ATP depletion during ischemia. However, this does not seem sufficient to mitigate early signs of IRI.
Article
Gastroenterology & Hepatology
Isabel M. A. Bruggenwirth, Otto B. van Leeuwen, Yvonne de Vries, Silke B. Bodewes, Jelle Adelmeijer, Janneke Wiersema-Buist, Ton Lisman, Paulo N. Martins, Vincent E. de Meijer, Robert J. Porte