Article
Environmental Sciences
Naeun Kim, Yongtae Ahn, Jungman Jo, Heesoo Pyo, Jeongae Lee, Jaeyoung Choi
Summary: This study investigated the effects of accidental contamination of soils with various chemicals, and found that phytosphingosine and Sphingomonas could potentially serve as biomarkers to evaluate soil contamination. Rain played an important role in the recovery of microbial and metabolic profiles after chemical accidents.
Review
Biochemistry & Molecular Biology
Yaroslav Shansky, Julia Bespyatykh
Summary: Bile acids are important in nutrient digestion and act as signaling molecules in the blood circulation. The gut microbiota plays a role in modifying the effects of bile acids. Depending on the composition of the microbiota, bile acids can support homeostasis or contribute to pathological conditions. Bile acids have potential as biomarkers for human pathology.
Article
Genetics & Heredity
Jinliang Peng, Chongrong Qiu, Jun Zhang, Xiaoliu Xiao
Summary: By using serum metabolomics, this study identified differential expression of metabolites in sepsis patients, which could potentially serve as diagnostic markers for sepsis. The pathogenesis of sepsis was found to be associated with mTOR signaling, NF-κB signaling pathway, calcium signaling, calcium transport, and tRNA charging pathway.
BMC MEDICAL GENOMICS
(2023)
Article
Gastroenterology & Hepatology
Zhen-Hua Ma, Xiao-Mei Wang, Rui-Hong Wu, Da-Lin Hao, Li-Chao Sun, Pan Li, Jun-Qi Niu
Summary: This study utilized ultra-high performance liquid chromatography-tandem mass spectrometry to analyze serum samples from patients with PBC, AIH, and healthy controls. It identified changes in levels of amino acids, bile acids, organic acids, phospholipids, choline, sugars, and sugar alcohols. Furthermore, it found that bile acids levels could serve as potential biomarkers for differentiating PBC from AIH.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Myriam Mireault, Vivaldy Prinville, Leanne Ohlund, Lekha Sleno
Summary: Using a semi-targeted approach, the effect of acetaminophen on circulating bile acid profiles in rats was investigated. The presence of known bile acids, potential isomeric structures, glucuronide and sulfate conjugates was examined using a reverse-phase method coupled to a high-resolution quadrupole time-of-flight system. Statistically significant changes in bile acid profiles were found between different doses of acetaminophen, with certain compounds showing significant increase at the highest dose, potentially indicating drug-induced liver injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Parasitology
Mingxing Zhu, Xiancai Du, Hongxia Xu, Songhao Yang, Chan Wang, Yazhou Zhu, Tingrui Zhang, Wei Zhao
Summary: Metabolomics approach revealed that the metabolism of nucleotides, alkaloids, amino acids, amides, and organic acids in mice is closely interrelated with E. granulosus infection. The study found that metabolic pathways in the liver such as tyrosine and tryptophan biosynthesis, phenylalanine, valine, leucine and isoleucine biosynthesis, and phenylalanine metabolism were notably associated with the occurrence and development of hydatid disease.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Minnie Jacob, Refat M. Nimer, Mohamad S. Alabdaljabar, Essa M. Sabi, Mysoon M. Al-Ansari, Maged Housien, Khalid M. Sumaily, Lina A. Dahabiyeh, Anas M. Abdel Rahman
Summary: This study used liquid chromatography-mass spectrometry (LC-MS) metabolomics to analyze the metabolome of serum from patients with nephrotic syndrome (NS) and found significant changes in 176 metabolites compared to the control group. Dysregulation of arginine, proline, and tryptophan metabolism, as well as arginine, phenylalanine, tyrosine, and tryptophan biosynthesis, were the most common metabolic pathways affected in NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Cheng Li, Zhiqian Liu, Carolyn Bath, Leah Marett, Jennie Pryce, Simone Rochfort
Summary: This paper presents an optimized protocol combining 3-NPH derivatization and LC-MS analysis to quantify SCFA and BHBA in bovine milk and serum. The method is sensitive, accurate, reproducible, and has a short sample preparation step. Applying this method to samples reveals significant variation in SCFA concentration between milk and serum, as well as strong correlations between some SCFAs in milk and serum samples.
Article
Immunology
Haiyan Gong, Sajid Ur Rahman, Keke Zhou, Zhibing Lin, Rongsheng Mi, Yan Huang, Yan Zhang, Yehua Zhang, Haiyan Jia, Wenqiang Tang, Chenyang Xia, Kishor Pandey, Zhaoguo Chen
Summary: In this study, non-targeted metabolomics was used to analyze the temporal dynamic changes in the metabolic profile of Babesia-infected erythrocytes. The results showed significant alterations in metabolites, providing new insights into the pathogenesis of Babesia and potential targets for vaccine/drug development.
MICROBIAL PATHOGENESIS
(2023)
Article
Pharmacology & Pharmacy
Shan Guo, Manting Lin, Di Xie, Wenqing Zhang, Mi Zhang, Li Zhou, Sheng Li, Hankun Hu
Summary: In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics analysis was conducted to comprehensively profile the metabolites in wild Chinese Cordyceps species and their substituents. A total of 901 metabolites were identified, and remarkable differences and significantly different metabolites were found among them. Quantitative analysis of amino acid-relevant metabolites was performed for the first time in four samples. The study provides valuable metabolic information for the quality control and optimal utilization of Cordyceps species.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Gastroenterology & Hepatology
Teng Liu, Ren-Xue Wang, Jun Han, Zhong-Die Li, Jonathan A. Sheps, Li -Juan Zheng, Xiao-Xiao Xu, Victor Ling, Jian-She Wang
Summary: Comprehensive bile acid profiling revealed increased bile acid modifications and decreased overall hydrophobicity in ABCB4-mutated patients. However, the concentration of tauro-THBA did not differ significantly among patients with different prognoses. ROC analysis indicated that tauro-THBA levels could be used as a prognostic marker for ABCB11-mutated patients.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ker Shien Tan, Adzzie Shazleen Azman, Pouya Hassandarvish, Zheng Hua Amelia-Yap, Tiong Kai Tan, Van Lun Low
Summary: This study used LC-MS/MS to investigate the protein profile changes in Aedes aegypti larvae and adults treated with lethal concentrations of Streptomyces sp. KSF103 ethyl acetate (EA) extract. The results showed upregulation of proteins associated with metabolism, protein regulation/degradation, energy production, cellular organization and structure, enzyme activity, and calcium ion transport and homeostasis. The study identified ATP synthase, fructose-bisphosphate aldolase (FBA), and ATP citrate synthase as potential protein targets for insecticide development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Domenico Masuero, Domen Skrab, Giulia Chitarrini, Mar Garcia-Aloy, Pietro Franceschi, Paolo Sivilotti, Graziano Guella, Urska Vrhovsek
Summary: A new mass spectrometry method was developed in this study, successfully analyzing 412 compounds in grapes. The results showed that certain lipid components were positively correlated with grape maturity, while others were negatively correlated.
Article
Immunology
Meilin Ding, Zha Zhen, Mei Ju, Suolang Quzong, Xuesi Zeng, Xiaoxia Guo, Rui Li, Mingming Xu, Jingjing Xu, Hongyang Li, Wei Zhang
Summary: Our study found significant differences in the metabolomic profiles between vitiligo patients and healthy subjects in both plateau and plain areas, with significantly higher levels of S1P in the serum of vitiligo patients and healthy subjects in the plateau area. These findings suggest that alterations in S1P metabolism may be involved in the pathogenesis of vitiligo.
CLINICAL IMMUNOLOGY
(2023)
Article
Physiology
Eden A. Houske, Matthew G. Glimm, Annika R. Bergstrom, Sally K. Slipher, Hope D. Welhaven, Mark C. Greenwood, Greta M. Linse, Ronald K. June, Alan S. L. Yu, Darren P. Wallace, Alyssa K. Hahn
Summary: This study analyzed the urine metabolites of early-stage ADPKD patients and identified metabolic pathway alterations and candidate diagnostic and prognostic biomarkers. The results provide potential therapeutic targets and biomarkers for early diagnosis and tracking disease progression of ADPKD.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)