An automated microfluidic gene-editing platform for deciphering cancer genes

Multi-size spheroid formation using microfluidic funnels

(2018) M. Marimuthu et al.   LAB ON A CHIP

Targeted-gene silencing of BRAF to interrupt BRAF/MEK/ERK pathway synergized photothermal therapeutics for melanoma using a novel FA-GNR-siBRAF nanosystem

(2018) Yujuan Zhang et al.   Nanomedicine-Nanotechnology Biology and Medicine

An Automated Induction Microfluidics System for Synthetic Biology

(2018) Mathieu C. Husser et al.   ACS Synthetic Biology

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding

(2017) Freeman Lan et al.   NATURE BIOTECHNOLOGY

Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis

(2017) Lindsey A Lonowski et al.   Nature Protocols

Microfluidic Cell Deformability Assay for Rapid and Efficient Kinase Screening with the CRISPR-Cas9 System

(2016) Xin Han et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Single cell functional analysis of multiple myeloma cell populations correlates with diffusion profiles in static microfluidic coculture systems

(2016) Thomas A. Moore et al.   Biomicrofluidics

Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX

(2016) Xin Yu et al.   BIOTECHNOLOGY LETTERS

Digital microfluidics for spheroid-based invasion assays

(2016) Brian F. Bender et al.   LAB ON A CHIP

Microfluidic Fabrication of Pluronic Vesicles with Controlled Permeability

(2016) Débora F. do Nascimento et al.   LANGMUIR

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Clusters of circulating tumor cells traverse capillary-sized vessels

(2016) Sam H. Au et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells

(2016) Scott D. Findlay et al.   PLoS One

Digital Microfluidic Cell Culture

(2015) Alphonsus H.C. Ng et al.   Annual Review of Biomedical Engineering

Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

(2015) Sidi Chen et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

MicroC3: an ex vivo microfluidic cis-coculture assay to test chemosensitivity and resistance of patient multiple myeloma cells

(2015) Chorom Pak et al.   Integrative Biology

Digital microfluidics for time-resolved cytotoxicity studies on single non-adherent yeast cells

(2015) P. T. Kumar et al.   LAB ON A CHIP

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

High-throughput functional genomics using CRISPR–Cas9

(2015) Ophir Shalem et al.   NATURE REVIEWS GENETICS

Identification and characterization of essential genes in the human genome

(2015) T. Wang et al.   SCIENCE

CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation

(2015) X. Han et al.   Science Advances

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

Targeting RAS–ERK signalling in cancer: promises and challenges

(2014) Ahmed A. Samatar et al.   NATURE REVIEWS DRUG DISCOVERY

Microgels on-demand

(2014) Irwin A. Eydelnant et al.   Nature Communications

Targeted genomic rearrangements using CRISPR/Cas technology

(2014) Peter S. Choi et al.   Nature Communications

PR-PR: Cross-Platform Laboratory Automation System

(2014) Gregory Linshiz et al.   ACS Synthetic Biology

Cellular bias on the microscale: probing the effects of digital microfluidic actuation on mammalian cell health, fitness and phenotype

(2013) Sam H. Au et al.   Integrative Biology

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy

(2013) Samuel A. Hasson et al.   NATURE

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

(2013) Hiroko Koike-Yusa et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

A vector-free microfluidic platform for intracellular delivery

(2013) A. Sharei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Size-variable droplet actuation by interdigitated electrowetting electrode

(2012) Jianfeng Chen et al.   APPLIED PHYSICS LETTERS

Digital microfluidics with impedance sensing for integrated cell culture andanalysis

(2012) Steve C.C. Shih et al.   BIOSENSORS & BIOELECTRONICS

Systematic identification of genomic markers of drug sensitivity in cancer cells

(2012) Mathew J. Garnett et al.   NATURE

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity

(2012) Jordi Barretina et al.   NATURE

Biofunctionalization of electrowetting-on-dielectric digital microfluidic chips for miniaturized cell-based applications

(2011) Daan Witters et al.   LAB ON A CHIP

A digital microfluidic platform for primary cell culture and analysis

(2011) Suthan Srigunapalan et al.   LAB ON A CHIP

Virtual microwells for digital microfluidic reagent dispensing and cell culture

(2011) Irwin A. Eydelnant et al.   LAB ON A CHIP

A New Angle on Pluronic Additives: Advancing Droplets and Understanding in Digital Microfluidics

(2011) Sam H. Au et al.   LANGMUIR

Mammalian cell transfection: the present and the future

(2010) Tae Kyung Kim et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Effect of electrode geometry on performance of EWOD device driven by battery-based system

(2009) Ling-Sheng Jang et al.   BIOMEDICAL MICRODEVICES

Optimization of device geometry in single-plate digital microfluidics

(2009) Mohamed Abdelgawad et al.   JOURNAL OF APPLIED PHYSICS

Microfluidic devices for cell based high throughput screening

(2009) Sarvesh Upadhyaya et al.   LAB ON A CHIP

Knocking down barriers: advances in siRNA delivery

(2009) Kathryn A. Whitehead et al.   NATURE REVIEWS DRUG DISCOVERY

Cross-scale electric manipulations of cells and droplets by frequency-modulated dielectrophoresis and electrowetting

(2008) Shih-Kang Fan et al.   LAB ON A CHIP

Pluronic Additives: A Solution to Sticky Problems in Digital Microfluidics

(2008) Vivienne N. Luk et al.   LANGMUIR

Metabolic gene regulation in a dynamically changing environment

(2008) Matthew R. Bennett et al.   NATURE