Journal
LAB ON A CHIP
Volume 18, Issue 14, Pages 2124-2133Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8lc00196k
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Funding
- NSF graduate research fellowship [1106401]
- National Institutes of Health [R21GM111584, R21CA220082, R01GM1234542]
- NSF's Research Triangle Materials Research Science and Engineering Center [DMR-1121107]
- Duke-Coulter Translational Partnership Grant Program
- National Science Foundation as part of the National Nanotechnology Coordinated Infrastructure (NNCI) [ECCS-1542015]
- NATIONAL CANCER INSTITUTE [R21CA220082] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM123542, R21GM111584] Funding Source: NIH RePORTER
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We demonstrate a hybrid microfluidic system that combines fluidic trapping and acoustic switching to organize an array of single cells at high density. The fluidic trapping step is achieved by balancing the hydrodynamic resistances of three parallel channel segments forming a microfluidic trifurcation, the purpose of which was to capture single cells in a high-density array. Next, the cells were transferred into adjacent larger compartments by generating an array of streaming micro-vortices to move the cells to the desired streamlines in a massively parallel format. This approach can compartmentalize single cells with efficiencies of approximate to 67% in compartments that have diameters on the order of approximate to 100 um, which is an appropriate size for single cell proliferation studies and other single cell biochemical measurements.
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