Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 80, Issue 6, Pages 1007-1011Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.18-0131
Keywords
antioxidant; developmental competence; embryo; oxidative stress; trans-ferulic acid
Categories
Funding
- Japan Science and Technology Agency/Japan International Cooperation Agency, Science and Technology Research Partnership for Sustainable Development (JST/JICA, SATREPS)
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The value of laboratory and genetically-modified pigs is becoming increasingly clear; however, their in vitro development remains inefficient. Trans-ferulic acid (trans-FA) is an aromatic compound that is abundant in plant cell walls, and which exhibits antioxidant effects in vitro. Trans-FA is known to improve sperm viability and motility; however, its effects on porcine oocytes are unknown. Our aim was to investigate the effects of trans-FA supplementation during in vitro maturation on the meiotic and developmental competence of porcine oocytes. Oocytes were matured either without (control) or with trans-FA (10, 100 and 1,000 mu M), fertilized, and cultured in vitro for 7 days. The maturation rate of oocytes cultured with 10 mu M trans-FA (81.6%) was significantly higher than that of controls (65.0%; P<0.05). The fertilization rate of oocytes matured with 10 mu M trans-FA (57.4%) was also significantly higher than that of controls (32.7%) and oocytes cultured with other concentrations (33.1% and 22.7% for 100 and 1,000 mu M, respectively; P<0.05). Moreover, the blastocyst formation rate of oocytes matured with 10 mu M trans-FA (6.9%) was significantly higher than that of controls (2.3%; P<0.05). Our results suggest that in vitro maturation with 10 mu M trans-FA is beneficial for the in vitro production of porcine embryos and has the potential to improve production system.
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