Journal
JOURNAL OF VETERINARY INTERNAL MEDICINE
Volume 32, Issue 3, Pages 1215-1233Publisher
WILEY
DOI: 10.1111/jvim.15095
Keywords
equine metabolic syndrome; horse; hyperinsulinemia; insulin resistance; metabolomics; oral sugar test
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Funding
- National Institutes of Health NIAMS
- University of Minnesota College of Veterinary Medicine
- University of Minnesota Equine Center
- Michigan State University College of Veterinary Medicine
- U.S. Department of Agriculture [2009-55205-05254]
- Morris Animal Foundation [D12EQ-028, D14EQ-033]
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [T32AR007612] Funding Source: NIH RePORTER
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BackgroundMetabolomics, the study of small-molecule metabolites, has increased understanding of human metabolic diseases, but has not been used to study equine metabolic syndrome (EMS). Objectives(1) To examine the serum metabolome of Welsh Ponies with and without insulin dysregulation before and during an oral sugar test (OST). (2) To identify differences in metabolites in ponies with insulin dysregulation, obesity, or history of laminitis. AnimalsTwenty Welsh Ponies (meanSD; 13.89.0 years) classified as non-insulin dysregulated [CON] (n=10, insulin<30 mU/L) or insulin dysregulated [ID] (n=10, insulin>60 mU/L) at 75 minutes after administration of Karo syrup, obese (n=6) or nonobese (n=14), and history of laminitis (n=9) or no history of laminitis (n=11). MethodsCase-control study. Metabolomic analysis was performed on serum obtained at 0 minutes (baseline) and 75 minutes during the OST. Data were analyzed with multivariable mixed linear models with significance set at P.05. ResultsMetabolomic analysis of 646 metabolites (506 known) detected significant metabolite differences. At baseline, 55 metabolites (insulin response), 91 metabolites (obesity status), and 136 metabolites (laminitis history) were different. At 75 minutes, 51 metabolites (insulin response), 102 metabolites (obesity status), and 124 metabolites (laminitis history) were different. Conclusions and Clinical ImportanceUse of metabolomics could have diagnostic utility for early detection of EMS and provide new knowledge regarding the pathophysiology of metabolic perturbations associated with this condition that might lead to improved clinical management.
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