Article
Multidisciplinary Sciences
Markus Seeger, Antonios Stylogiannis, Ludwig Prade, Sarah Glasl, Vasilis Ntziachristos
Summary: By using overdriving continuous-wave laser diodes and frequency-wavelength multiplexing based on illumination pulse-trains, we demonstrate concurrent multi-wavelength optoacoustic microscopy with high signal-to-noise ratios and high resolution, allowing visualization of vascular oxygenation dynamics and circulating tumor cells.
SCIENTIFIC REPORTS
(2023)
Article
Optics
Ruihong Dai, Nan Zhang, Yafei Meng, Zhenqiao Zhou, Fengqiu Wang
Summary: A compact and high-energy femtosecond fiber laser system based on CPA and SHG technology has been demonstrated, capable of generating high-energy, short-pulse laser with tunable wavelength. The system ensures pulse width of about 100 fs and sufficient pulse energy over the entire tuning range, performing well in a two-photon microscope.
Article
Engineering, Electrical & Electronic
Lingxiao Yang, Jaena Park, Marina Marjanovic, Eric J. Chaney, Darold R. Spillman, Heidi Phillips, Stephen A. Boppart
Summary: Intraoperative imaging in surgical oncology can provide valuable information about the tumor microenvironment and margin by visualizing microstructural features and molecular dynamics. Most current techniques use exogenous labels, which may disrupt the natural environment and alter the optical signatures. Portable nonlinear imaging systems have enabled real-time detection and diagnosis of tissue during surgery, showing potential for assisting in cancer diagnosis.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Optics
Xiaomin Zheng, Jie Zhou, Lei Wang, Meiting Wang, Wenshuai Wu, Jiajie Chen, Junle Qu, Bruce Zhi Gao, Yonghong Shao
Summary: Structured Illumination Microscopy (SIM) has shown remarkable progress in resolution, imaging depth, and technological advancements in recent years, making it a powerful tool in biomedical research. Its flexibility allows for effective combination with other methods, offering enhanced imaging capabilities.
Article
Urology & Nephrology
Stefano Gobbo, Albino Eccher, Sebastian Gallina, Damiano D'aietti, Alessandro Princiotta, Francesco Ditonno, Alessandro Tafuri, Maria A. Cerruto, Stefano Marletta, Francesca Sanguedolce, Aldo Scarpa, Matteo Brunelli, Alessandro Antonelli
Summary: The study validates the use of fluorescence laser confocal microscopy (FCM) for real-time cancer detection in prostate biopsies, demonstrating its effectiveness in diagnosing cancer cells.
MINERVA UROLOGY AND NEPHROLOGY
(2023)
Article
Medicine, Research & Experimental
Defu Chen, David W. Nauen, Hyeon-Cheol Park, Dawei Li, Wu Yuan, Ang Li, Honghua Guan, Carmen Kut, Kaisorn L. Chaichana, Chetan Bettegowda, Alfredo Quinones-Hinojosa, Xingde Li
Summary: This study systematically investigated the feasibility of using multiphoton microscopy for rapid histopathological assessment of label-and processing-free surgical specimens, focusing on infiltrating glioma. The results showed that multiphoton microscopy robustly captures diagnostic features of glioma and deep learning can assist with image recognition.
Article
Biochemical Research Methods
B. Talone, M. Bazzarelli, A. Schirato, F. Dello Vicario, D. Viola, E. Jacchetti, M. Bregonzio, M. T. Raimondi, G. Cerullo, D. Polli
Summary: This research experimentally characterized cell damage induced by femtosecond near-infrared laser pulses, providing a safety limit for nonlinear optical microscopy and demonstrating that imaging with high irradiances can avoid photodamage.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Optics
Kong Gao, Yizhou Liu, Wenchao Qiao, Yuzhi Song, Xian Zhao, Aimin Wang, Tao Li
Summary: In this study, an ytterbium-doped stretched-pulse mode-locked fiber oscillator was fabricated using a nonlinear amplifier loop mirror (NALM). The central wavelength of the laser can be modified by tuning the intra-cavity loss. The laser achieved high output power and short compressed pulse durations.
Article
Optics
Yueqiang Zhu, Chen Zhang, Yanyan Gong, Wei Zhao, Jintao Bai, Kaige Wang
Summary: The investigation introduces a method utilizing SSP for generating independent multifocal spots through a high-NA lens, validated through numerical simulation and experimental results indicating high uniformity and consistency.
Article
Chemistry, Physical
Alexander Mikhaylov, Ryan N. Wilson, Kristen M. Parzuchowski, Michael D. Mazurek, Charles H. Camp, Martin J. Stevens, Ralph Jimenez
Summary: The research found that a signal similar to entangled two-photon absorption (E2PA) can be observed under the hot-band absorption (HBA) mechanism. Under typical conditions for E2PA measurements, contributions from the HBA process could lead to an overestimation of the quantum advantage by several orders of magnitude.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Article
Multidisciplinary Sciences
Peter R. Corridon
Summary: This study examines the microarchitecture of acellular kidney scaffolds using intravital microscopy (IVM) data. The effectiveness of decellularization and the performance of acellular nephron compartments after transplantation were evaluated. The results contribute to the broader understanding and application of this imaging technique in analyzing scaffold systems.
Article
Archaeology
M. Martinez-Weinbaum, L. Maestro-Guijarro, P. M. Carmona-Quiroga, S. Siano, D. Ciofini, M. Castillejo, M. Oujja
Summary: This study investigates the extent of degradation of varnish coatings at different depths due to aging. By determining factors such as varnish type, aging time, and initial thickness, we can assess the degree of varnish deterioration non-invasively.
JOURNAL OF CULTURAL HERITAGE
(2023)
Article
Oncology
Adrien Villard, Ingrid Breuskin, Odile Casiraghi, Safaa Asmandar, Corinne Laplace-Builhe, Muriel Abbaci, Antoine Moya Plana
Summary: Confocal laser endomicroscopy and ex vivo confocal microscopy are promising technologies for real-time histological visualization of living tissue. They have been used for clinical diagnosis and tumor margin assessment, but there are still challenges to be addressed before their widespread clinical use.
Review
Physics, Multidisciplinary
Huiling Zhan, Caihong Sun, Mingyu Xu, Tianyi Luo, Guangxing Wang, Gangqin Xi, Zhiyi Liu, Shuangmu Zhuo
Summary: Novel fast microscopy imaging technologies such as SRS, PAM, MPM, and OCM have broad application prospects in tumor resection surgeries, offering more advantages compared to traditional methods. The proposed multi-mode hybrid detection technology combined with deep learning-based artificial intelligence can serve as the basis for future intraoperative diagnosis.
FRONTIERS IN PHYSICS
(2022)
Article
Dermatology
S. Springer, M. Zieger, K. Koenig, M. Kaatz, J. Lademann, M. E. Darvin
SKIN RESEARCH AND TECHNOLOGY
(2016)
Article
Dermatology
S. Springer, M. Zieger, A. Boettcher, J. Lademann, M. Kaatz
SKIN RESEARCH AND TECHNOLOGY
(2017)
Article
Dermatology
S. Springer, M. Zieger, U. C. Hipler, J. Lademann, V Albrech, R. Bueckle, C. Mess, M. Kaatz, V Huck
SKIN RESEARCH AND TECHNOLOGY
(2019)
Article
Dermatology
S. Springer, M. Zieger, U. C. Hipler, K. Koenig, J. Lademann, M. Kaatz, M. J. Koehler
SKIN RESEARCH AND TECHNOLOGY
(2018)
Letter
Immunology
Steffen Springer, Lisa M. Menzel, Michael Zieger
BRAIN BEHAVIOR AND IMMUNITY
(2020)
Letter
Immunology
Steffen Springer, Lisa M. Menzel, Michael Zieger
BRAIN BEHAVIOR AND IMMUNITY
(2020)
Letter
Rheumatology
Steffen Springer, Artur Strzelecki, Michael Zieger
CLINICAL RHEUMATOLOGY
(2021)
Article
Dermatology
Michael Zieger, Martin Kaatz, Steffen Springer, Rainer Riesenberg, Andreas Wuttig, Mario Kanka, Sarmiza Stanca, Carina Reble, Georg Khazaka, Robin Sieg, Marco De Gregorio, Martin Sattler, Frank Fischer
Summary: The handheld device using laser speckle imaging technology was developed and qualified for in vivo human skin evaluation. The prototypes allow for selection of up to three different laser wavelengths and provide various speckle parameters for skin analysis. Results showed reproducible data for skin evaluation, demonstrating the device's potential for practical application.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Steffen Springer, Michael Zieger, Artur Strzelecki
Summary: This study found that, as interest in topics such as infoveillance, infodemiology and Google Trends increased during the initial stages of the pandemic, the number of published papers on these subjects also saw an uptick.
Article
Biodiversity Conservation
Branden Holmes, Artur Strzelecki, Steffen Springer, Michael Zieger
Summary: In nature conservation, generating public interest, attention, and emotions is crucial for the protection of nature, biotopes, and species. Charismatic flagship species play a significant role in this process. This study analyzed vertebrate species from various classes and identified traits that make a species a flagship species, such as belonging to Mammalia, having a large body mass, and a carnivorous diet. The study also found that mammalian species received the most attention, and species with a higher body mass were overrepresented. These popular species can be selected as ambassadors for conservation to protect entire biomes and benefit less charismatic species.
ENVIRONMENTAL CONSERVATION
(2022)
Article
Immunology
Steffen Springer, Martin Kaatz, Michael Zieger
Summary: Global efforts are underway to end the COVID-19 pandemic, with Germany focusing on providing vaccines to as many people as possible after lifting prioritization. A study using data from the Robert Koch Institute found a negative correlation between vaccination rate and infection incidence in 16 German states, indicating a higher vaccination rate corresponds to a lower infection rate during the 4th wave.
Article
Immunology
Martin Kaatz, Steffen Springer, Roger Schubert, Michael Zieger
Summary: This study used Google Trends data to track public awareness of long COVID and its important symptoms during the COVID-19 pandemic. The results show a clear correlation between public interest and search volume, with an increased attention during the pandemic. Therefore, Google Trends is a useful tool to measure the population's awareness of infodemiological topics like long COVID.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2022)
Article
Biodiversity Conservation
Michael Zieger, Steffen Springer
Summary: This study compared the interest in charismatic species with other terms related to nature and environmental protection, using Google Trends. The results did not clearly demonstrate a general benefit or necessity for charismatic species to generate interest in conservation and environmental issues.
PACIFIC CONSERVATION BIOLOGY
(2021)
Article
Zoology
Michael Zieger, Steffen Springer
AUSTRALIAN JOURNAL OF ZOOLOGY
(2019)
