Review
Biochemistry & Molecular Biology
Panagiotis Theofilis, Marios Sagris, Alexios S. Antonopoulos, Evangelos Oikonomou, Costas Tsioufis, Dimitris Tousoulis
Summary: In the context of dysfunctional vascular endothelium, platelet activation occurs due to structural alterations like endothelial glycocalyx thinning or functional modifications through impaired NO bioavailability. Additionally, neutrophil-derived mediators and neutrophil extracellular traps formation contribute to platelet activation and platelet-leukocyte aggregation. Pro-inflammatory cytokines and their receptors in platelets, along with factors like TNF-alpha, play critical roles in inducing inflammatory responses and affecting platelet activation. Furthermore, new concepts linking inflammation and platelet activation, such as toll-like receptors, myeloperoxidase, and platelet factor-4, have been explored. The evidence is particularly relevant in the context of COVID-19, where excessive inflammation leads to thrombotic complications partially mediated by platelet activation. Advances in anti-inflammatory therapies have shown promise in reducing platelet activation and its thrombotic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Muataz Ali Hamad, Krystin Krauel, Nancy Schanze, Nadine Gauchel, Peter Stachon, Thomas Nuehrenberg, Mark Zurek, Daniel Duerschmied
Summary: In addition to their role in hemostasis and thrombosis, platelets also play a role in modulating inflammatory reactions and immune responses. Circulating platelets can differ within an individual due to their different physical properties and can be categorized into distinct subtypes based on their function or age. Understanding platelet phenotypic variation is important in various physiological and pathological circumstances.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Anne Strohbach, Friedemann Maess, Katharina Wulf, Svea Petersen, Niels Grabow, Klaus-Peter Schmitz, Stephan B. Felix, Raila Busch
Summary: The main goal of new stent technologies is to produce bio- and hemo-compatible polymers with anti-inflammatory and anti-thrombogenic properties to overcome unfavorable material-related incompatibilities. Wettability is a crucial surface property affecting the biological response of blood cells, while local hemodynamic changes also impact blood cell activation. High shear rates may not be accurately predicted by wettability alone, suggesting extensive in vitro testing is necessary for blood compatibility assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Prathap Jayaram, Parker J. T. Mitchell, Theodore B. Shybut, Bruce J. Moseley, Brendan Lee
Summary: This study investigated the effects of two different types of platelet-rich plasma (PRP) on pain and inflammation in patients with mild to moderate knee osteoarthritis. The results showed that leukocyte-rich PRP (LR-PRP) contained more anti-inflammatory factors compared to leukocyte-poor PRP (LP-PRP), but there were no significant differences in pain regulation between the two.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2023)
Article
Cell Biology
Fanny Vardon-Bounes, Cedric Garcia, Alexandra Piton, Jennifer Series, Marie-Pierre Gratacap, Michael Poette, Thierry Seguin, Laure Crognier, Stephanie Ruiz, Stein Silva, Jean-Marie Conil, Vincent Minville, Bernard Payrastre
Summary: This study assessed platelet activation parameters at admission and 48 hours later in patients with sepsis, revealing significantly increased platelet activation levels in patients compared to controls at both time points, but with less responsiveness to different agonists.
Article
Hematology
Anna Schmuckenschlager, Anita Pirabe, Alice Assinger, Waltraud C. Schrottmaier
Summary: Platelets play a crucial role in hemostasis and also regulate immune responses by interacting with leukocytes. They are highly sensitive to micro-environmental changes, which is important under physiological conditions but can be problematic in vitro.
THROMBOSIS RESEARCH
(2023)
Article
Medicine, Research & Experimental
Clara Ruiz-Fernandez, Maria Gonzalez-Rodriguez, Vanessa Abella, Vera Francisco, Alfonso Cordero-Barreal, Djedjiga Ait Eldjoudi, Yousof Farrag, Jesus Pino, Javier Conde-Aranda, Miguel Angel Gonzalez-Gay, Antonio Mera, Ali Mobasheri, Lucia Garcia-Caballero, Marina Gandara-Cortes, Francisca Lago, Morena Scotece, Oreste Gualillo
Summary: WISP-2, a recently identified adipokine, plays a role in modulating the turnover of extracellular matrix in the cartilage, and its downregulation may detrimentally alter the inflammatory environment in osteoarthritic cartilage. The authors also show the participation of Wnt/beta-catenin signaling pathway in these processes. Thus, targeting WISP-2 might represent a potential therapeutical approach for degenerative and/or inflammatory diseases of musculoskeletal system, such as osteoarthritis.
LABORATORY INVESTIGATION
(2022)
Article
Hematology
Ana Kasirer-Friede, Emilia Peuhu, Johanna Ivaska, Sanford J. Shattil
Summary: This study demonstrates the involvement of SHARPIN in regulating important functions of platelets, including inflammation and integrin adhesion. Platelets lacking SHARPIN showed enhanced spreading and adhesion on fibrinogen, but compromised thrombus growth on collagen. The loss of SHARPIN also resulted in reduced NF-kappa B activation and linear ubiquitination of protein substrates in platelets, and significantly reduced inflammation in mouse models.
Article
Immunology
Jue Hou, Shuhui Wang, Dan Li, Lindsay N. Carpp, Tong Zhang, Ying Liu, Manxue Jia, Hong Peng, Chang Liu, Hao Wu, Yunda Huang, Yiming Shao
Summary: In a study on HIV-1 vaccination, researchers found that some participants did not generate an anti-vaccinia neutralizing antibody response despite successful vaccination, while others showed downregulation of the AP-1 pathway and upregulation of T-cell activation in responders. Additionally, the analysis revealed a temporary upregulation of AP-1 core genes followed by downregulation in responders, while non-responders exhibited an upregulation of AP-1 core and pro-inflammatory genes, suggesting that early pro-inflammatory signaling may inhibit the neutralizing antibody response post-vaccination.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Yuuki Ohara, Paloma Valenzuela, S. Perwez Hussain
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive tumor type, with development intricately linked to inflammatory signaling and metabolic reprogramming. Understanding the interaction of these factors may provide new insights for the treatment of PDAC.
Article
Toxicology
Felipe Silva de Franca, Joel Jose Megale Gabrili, Laurence Mathieu, Francois Burgher, Joel Blomet, Denise V. Tambourgi
Summary: The study investigated the systemic events in human whole-blood model induced by Bothrops lanceolatus venom, revealing a strong inflammatory reaction characterized by complement activation and release of lipid mediators that may favor thrombi formation.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ornella Bosco, Barbara Vizio, Gabriella Gruden, Martina Schiavello, Bartolomeo Lorenzati, Paolo Cavallo-Perin, Isabella Russo, Giuseppe Montrucchio, Enrico Lupia
Summary: Increased THPO levels in diabetic patients are associated with higher platelet activation, including elevated platelet-monocyte and platelet-granulocyte binding, as well as platelet P-selectin expression. THPO concentrations correlate with platelet-leukocyte binding, fasting glucose, and Hb1Ac levels. In vitro studies show that the priming effect of diabetic patient plasma on platelet-leukocyte binding and P-selectin expression can be prevented by blocking THPO activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Christina C. Rolling, Tessa J. Barrett, Jeffrey S. Berger
Summary: Platelets, as vital components in primary hemostasis and thrombosis, have been recognized as important cellular mediators in innate immunity and inflammation. Through forming monocyte-platelet aggregates (MPA) with circulating monocytes, activated platelets have the ability to modulate the phenotype and function of monocytes. The platelet-monocyte axis serves as a crucial link between thrombosis and inflammation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Plant Sciences
Jiang Jiang, Dengping Liu, Yuanyuan Wang, Wei Li, Zhihui Hong, Jianzhong An, Shigang Qiao, Zhanli Xie
Summary: The research found that GLA can alleviate liver dysfunction in the mouse model of sepsis. GLA-treated mice displayed lower complement activation and liver dysfunction after E. coli infection. GLA inhibited platelet activation through the RIP1/RIP3/AKT pathway and downregulated C3aR expression on the platelets, thereby inhibiting liver injury and dysfunction due to excessive complement activation.
Article
Hematology
Csaba Loibl, Martin Rozanovic, Lajos Bogar, Andrea Pankaczi, Patricia Kovacs, Attila Miseta, Tihamer Molnar, Csaba Csontos
Summary: The study found that the platelet and leukocyte antisedimentation rate (PAR and LAR) continuously increased in burn injury patients over a five-day period. It also showed that lower PAR and LAR values were associated with ICU non-survivors and septic patients. The results suggest that PAR and LAR could be potential predictors of septic complications and poor outcomes in major burn injury cases. Further research with larger sample sizes is needed to confirm these findings.
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
(2021)
Article
Cardiac & Cardiovascular Systems
Peter Stachon, Klaus Kaier, Philip Hehn, Alexander Peikert, Dennis Wolf, Vera Oettinger, Dawid Staudacher, Daniel Duerschmied, Andreas Zirlik, Manfred Zehender, Christoph Bode, Constantin von zur Muehlen
Summary: In a nationwide registry study in Germany, left main stenting was found to be a safe and effective treatment option for chronic coronary syndrome patients with left main coronary artery disease, showing reduced risks of bleeding, prolonged ventilation time, and postoperative delirium after risk adjustment, along with shorter hospital stay and lower costs.
CLINICAL RESEARCH IN CARDIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Marina Rieder, Nadine Gauchel, Klaus Kaier, Carolin Jakob, Stefan Borgmann, Annika Y. Classen, Jochen Schneider, Lukas Eberwein, Martin Lablans, Maria Ruethrich, Sebastian Dolff, Kai Wille, Martina Haselberger, Hanno Heuzeroth, Christoph Bode, Constantin von Zur Muehlen, Siegbert Rieg, Daniel Duerschmied
Summary: The presence of pre-existing oral anticoagulation (OAC) in COVID-19 patients has been shown to have a protective influence on mortality and recovery rates, as well as reduce the frequency of thrombotic events. This effect is independent of the anticoagulation regime during hospital stay and the stage of disease.
CLINICAL RESEARCH IN CARDIOLOGY
(2022)
Article
Immunology
Juliana de Castilhos, Eli Zamir, Theresa Hippchen, Roman Rohrbach, Sabine Schmidt, Silvana Hengler, Hanna Schumacher, Melanie Neubauer, Sabrina Kunz, Tonia Mueller-Esch, Andreas Hiergeist, Andre Gessner, Dina Khalid, Rogier Gaiser, Nyssa Cullin, Stamatia M. Papagiannarou, Bettina Beuthien-Baumann, Alwin Kraemer, Ralf Bartenschlager, Dirk Jaeger, Michael Mueller, Felix Herth, Daniel Duerschmied, Jochen Schneider, Roland M. Schmid, Johann F. Eberhardt, Yascha Khodamoradi, Maria J. G. T. Vehreschild, Andreas Teufel, Matthias P. Ebert, Peter Hau, Bernd Salzberger, Paul Schnitzler, Hendrik Poeck, Eran Elinav, Uta Merle, Christoph K. Stein-Thoeringer
Summary: The study found that severe cases of COVID-19 can lead to dysbiotic microbial configurations in the oropharyngeal microbiome, particularly in patients treated with broad-spectrum antibiotics, receiving invasive mechanical ventilation, or with prolonged hospitalization. Early specimen collection allowed for the prediction of COVID-19 mortality using machine learning models, with taxonomic signatures outperforming models based on clinical variables, with Neisseria and Haemophilus species abundances being the most important features.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Hematology
Maximilian Mauler, Nancy Schanze, Krystin Krauel, Claudia Schoenichen, Franziska Glatzki, Susanna Poeschl, Daniela Stallmann, Julius Mezger, Nadine Gauchel, Diana Sharipova, Marina Rieder, Ingo Hilgendorf, Thilo Witsch, Christoph Bode, Daniel Duerschmied
Summary: Peripheral serotonin seems to have no effect on endothelial adhesion molecule expression, despite its influence on neutrophil function. The inflammatory release of peripheral serotonin appears to be unnecessary for regulating endothelial adhesion molecules.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Cardiac & Cardiovascular Systems
Nadine Gauchel, Marina Rieder, Krystin Krauel, Isabella Goller, Maren Jeserich, Ulrich Salzer, Ana Cecilia Venhoff, Niklas Baldus, Luisa Pollmeier, Luisa Wirth, Winfried Kern, Siegbert Rieg, Hans-Jorg Busch, Maike Hofmann, Christoph Bode, Daniel Duerschmied, Achim Lother, Lukas A. Heger
Summary: This study compared the levels of complement system components in COVID-19 patients and other groups, revealing that COVID-19 patients had significantly elevated levels of CS components 5a and 4, increased levels of vWF antigen, and a significant correlation with vWF antigen, suggesting a pronounced immune inflammatory response in COVID-19 disease.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2022)
Article
Cardiac & Cardiovascular Systems
Uzair Ansari, Simone Britsch, Sebastian Rogowski, Daniel Duerschmied, Theano Papavassiliu
Summary: We present a case of acute myocarditis reported after COVID-19 vaccination. CMR is useful for diagnosis and follow-up of myocarditis patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Hematology
Nancy Schanze, Muataz Ali Hamad, Thomas Georg Nuehrenberg, Christoph Bode, Daniel Duerschmied
Summary: Coronary artery disease is a leading cause of global mortality, with ischemia/reperfusion injury (IRI) contributing to cardiac damage. Platelets play an important role in IRI through activation and interaction with other cells. Understanding platelet contributions to IRI is crucial for developing effective treatment strategies.
Article
Biochemistry & Molecular Biology
Xuehui Fan, Lukas Cyganek, Katja Nitschke, Stefanie Uhlig, Philipp Nuhn, Karen Bieback, Daniel Duerschmied, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin
Summary: This study compared the electrophysiological and functional properties of endothelial cells derived from human induced pluripotent stem cells with primary human cardiac microvascular endothelial cells. The results demonstrated that the two types of endothelial cells were similar in terms of ion channel function, membrane receptor signaling, and physiological cell functions, although some differences were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Stephanie Luise Rosenkaimer, Laura Winter, Tina Sieburg, Sandra Maier, Athanasios Mavratzas, Wolf-Karsten Hofmann, Ibrahim Akin, Daniel Duerschmied, Ralf-Dieter Hofheinz, Anna Hohneck
Summary: This exploratory study investigated the diagnostic value of inflammatory markers in predicting anti-tumour treatment-related cardiac events in patients with breast cancer. The results showed that markers such as VCAM-1 and sST2 were associated with an increased likelihood of treatment-related events, providing potential for better identification of high-risk patients.
ONCOLOGY RESEARCH AND TREATMENT
(2022)
Article
Hematology
Stefano Barco, Davide Voci, Ulrike Held, Tim Sebastian, Roland Bingisser, Giuseppe Colucci, Daniel Duerschmied, Andre Frenk, Bernhard Gerber, Andrea Goetschi, Stavros Konstantinides, Francois Mach, Helia Robert-Ebadi, Thomas Rosemann, Noemi R. Simon, Herve Spechbach, David Spirk, Stefan Stortecky, Lukas Vaisnora, Marc Righini, Nils Kucher
Summary: This study aimed to investigate whether thromboprophylaxis with enoxaparin would prevent hospitalization and death in symptomatic COVID-19 outpatients. The results showed that enoxaparin did not reduce the risk of hospitalization and death among these patients.
LANCET HAEMATOLOGY
(2022)
Article
Medicine, General & Internal
Diona Gjermeni, Hannah Vetter, Sofia Szabo, Viktoria Anfang, Stefan Leggewie, David Hesselbarth, Daniel Duerschmied, Dietmar Trenk, Christoph B. Olivier
Summary: This study compared the performance of multiple platelet aggregometry (MEA) and thrombelastography (TEG) in detecting high on-clopidogrel platelet reactivity (HPR) in patients with atrial fibrillation (AF) and indication for oral anticoagulation (OAC) after percutaneous coronary intervention (PCI). The results showed that TEG detected higher rates of HPR compared to MEA. Conventional cut-off values for HPR may need to be re-evaluated for this high-risk population. Further studies are needed to assess the association with outcomes.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Anna C. Mavromanoli, Stefano Barco, Walter Ageno, Helene Bouvaist, Marianne Brodmann, Claudio Cuccia, Francis Couturaud, Claudia Dellas, Konstantinos Dimopoulos, Daniel Duerschmied, Klaus Empen, Pompilio Faggiano, Emile Ferrari, Nazzareno Galie, Marcello Galvani, Alexandre Ghuysen, George Giannakoulas, Menno Huisman, David Jimenez, Matija Kozak, Irene M. Lang, Nicolas Meneveau, Thomas Muenzel, Massimiliano Palazzini, Antoniu Octavian Petris, Giancarlo Piovaccari, Aldo Salvi, Sebastian Schellong, Kai-Helge Schmidt, Franck Verschuren, Irene Schmidtmann, Gerrit Toenges, Frederikus A. Klok, Stavros Konstantinides
Summary: This study analyzed the changes in RV function in patients with intermediate-risk PE who were switched early to oral anticoagulation. The results showed that the majority of patients had normalized RV function within 6 days and maintained normal RV function throughout the first 6 months. However, almost one in four patients continued to have evidence of RV dysfunction over the long term.
CLINICAL RESEARCH IN CARDIOLOGY
(2023)
Article
Hematology
Davide Voci, Andrea Gotschi, Ulrike Held, Roland Bingisser, Giuseppe Colucci, Daniel Duerschmied, Riccardo M. Fumagalli, Bernhard Gerber, Barbara Hasse, Dagmar I. Keller, Stavros V. Konstantinides, Francois Mach, Silvana K. Rampini, Marc Righini, Helia Robert-Ebadi, Thomas Rosemann, Stephanie Roth-Zetzsche, Tim Sebastian, Noemi R. Simon, David Spirk, Stefan Stortecky, Lukas Vaisnora, Nils Kucher, Stefano Barco
Summary: The benefits of early thromboprophylaxis in symptomatic COVID-19 outpatients remain unclear. This study presents the 90-day results from the OVID phase III trial, which showed that early thromboprophylaxis with enoxaparin did not improve the course of COVID-19 in terms of hospitalization, death, or resolution of symptoms.
THROMBOSIS RESEARCH
(2023)
Review
Virology
Aydin Huseynov, Ibrahim Akin, Daniel Duerschmied, Ruediger E. Scharf
Summary: An increasing number of post-COVID patients are experiencing long-term complications from the acute COVID-19 infection, posing ongoing challenges for healthcare professionals. It has become clear that COVID-19 not only affects the respiratory system but also other organs, including the cardiovascular system. Cardiopulmonary symptoms such as shortness of breath, palpitations, limited physical capacity, and cardiac arrhythmias can persist in a significant proportion of recovered patients. This article reviews the prevalence and underlying mechanisms of COVID-19-associated arrhythmias and discusses the clinical management of various rhythm disorders in post-COVID patients.
Article
Hematology
Marina Rieder, Niklas Baldus, Daniela Stallmann, Maren Jeserich, Isabella Goller, Luisa Wirth, Luisa Pollmeier, Maike Hofmann, Christoph Bode, Hans-Joerg Busch, Bonaventura Schmid, Nadine Gauchel, Rudiger E. Scharf, Daniel Duerschmied, Achim Lother, Krystin Krauel
Summary: This study aimed to assess platelet reactivity in patients with moderate COVID-19 at early stages. Platelet reactivity was evaluated using whole-blood impedance aggregometry and flow cytometry in 20 COVID-19-positive patients, 55 COVID-19-negative patients (as patient controls), and 15 healthy individuals.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
