Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume 9, Issue 11, Pages 2967-2971Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.8b00953
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Funding
- IISER Bhopal
- Wellcome Trust/DBT India Alliance award [IA/I/14/1/501305]
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Membrane protein aggregation is associated with neurodegenerative diseases. Despite remarkable advances to map protein aggregation, molecular elements that drive the structural transition from functional to amyloidogenic beta-sheet polymers remain elusive. Here, we report a simple and reliable reverse-mapping method to identify the molecular elements. We validate our approach by obtaining molecular details of aggregation loci of human beta-barrel nanopore ion channels that are vital for cell survival. By coupling bottom-up synthesis with time-resolved aggregation kinetics and high-resolution imaging, we identify molecular elements that switch folded channels to polymeric beta-rich aggregates. We prove that intrinsic protein aggregation and amyloidogenicity does not depend on total hydrophobicity but on single residue differences in the primary sequence. Our method offers effective strategies for sequence based design of aggregation inhibitors in biomedicine for neurodegenerative diseases.
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