Article
Biochemistry & Molecular Biology
Apparao B. Kummarapurugu, Shuo Zheng, Abigail Pulsipher, Justin R. Savage, Jonathan Ma, Bruce K. Rubin, Thomas P. Kennedy, Judith A. Voynow
Summary: In this study, the synthetic compound GM-1111 showed effective anti-NE activity in CF sputum, facilitating DNA depolymerization and reducing sputum elasticity. These findings suggest that GM-1111 could be a promising therapeutic option for CF patients, with potential advantages over traditional unfractionated heparin.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Infectious Diseases
Margherita Degasperi, Riccardo Sgarra, Mario Mardirossian, Sabrina Pacor, Massimo Maschio, Marco Scocchi
Summary: This study developed a prodrug to extend the peptide chain of an antimicrobial peptide and added a sequence that can activate the peptide at the site of infection/inflammation, enabling a slow and targeted release. The prodrug showed good antimicrobial activity and lower cytotoxicity in the presence of elastase.
Article
Medical Laboratory Technology
Lisa M. Johnson, Michael Spannagl, Nathalie Wojtalewicz, Juergen Durner
Summary: The study found that there is high variability in quantitative results between different manufacturers in fecal calprotectin assays, but qualitative interpretation in fecal pancreatic elastase assays seems to be harmonized across different manufacturers.
CLINICAL BIOCHEMISTRY
(2022)
Article
Immunology
Margalida Mateu-Borras, Alex Gonzalez-Alsina, Antonio Domenech-Sanchez, Javier Querol-Garcia, Francisco J. Fernandez, Ma Cristina Vega, Sebastian Alberti
Summary: This study investigates how isolates from CF patients modulate the levels of C5a and identifies the bacterial factors involved. The results show that most isolates from airway chronic infections induce the production and accumulation of C5a, which is attributed to the loss of C5a cleavage by the exoproteases AprA and LasB. Furthermore, it is found that lack of bacterial protease-dependent C5a degradation is due to mutations in the master regulator LasR. These findings suggest that non-cleaving C5a phenotype acquired by LasR variants may contribute to the strong neutrophilia and neutrophil dysfunction in CF patients.
Article
Immunology
Nina Erdmann, Theresa Schilling, Julia Hentschel, Thomas Lehmann, Philipp von Bismarck, Tobias Ankermann, Franziska Duckstein, Michael Baier, Carlos Zagoya, Jochen G. Mainz
Summary: This study compared upper airway inflammation and pathogen colonization during stable phases and exacerbations in CF patients and healthy controls. The study found that CF patients had weaker inflammatory responses compared to healthy controls during both stable and exacerbation phases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Respiratory System
Camilla Margaroli, Hamed Horati, Luke W. Garratt, Vincent D. Giacalone, Craig Schofield, A. Susanne Dittrich, Tim Rosenow, Brian S. Dobosh, Hong S. Lim, Dario L. Frey, Mieke Veltman, George L. Silva, Milton R. Brown, Carsten Schultz, Harm A. W. M. Tiddens, Sarath Ranganathan, Joshua D. Chandler, Peng Qiu, Limin Peng, Bob J. Scholte, Marcus A. Mall, Anthony Kicic, Lokesh Guglani, Stephen M. Stick, Hettie M. Janssens, Rabindra Tirouvanziam
Summary: This study found that upregulation of PD-1 is associated with airway macrophage exhaustion, neutrophil takeover, infection, and structural damage in children with cystic fibrosis.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Immunology
Marilena Pariano, Claudio Costantini, Ilaria Santarelli, Matteo Puccetti, Stefano Giovagnoli, Vincenzo N. Talesa, Luigina Romani, Cinzia Antognelli
Summary: Cystic fibrosis (CF) is an autosomal recessive disorder characterized by a decline in respiratory function. Research suggests that GLO1 plays a pathological role in CF and its activity can be restored by blocking interleukin-1 receptor signaling.
Article
Multidisciplinary Sciences
Ting Yang, Jinlong Yu, Tasdiq Ahmed, Katherine Nguyen, Fang Nie, Rui Zan, Zhiwei Li, Pei Han, Hao Shen, Xiaonong Zhang, Shuichi Takayama, Yang Song
Summary: Researchers study the antibacterial activity of synthetic NET-mimetic materials and find that the incorporation of NE into DNA-histone complexes reduces antimicrobial activity, but this effect can be reversed by treatment with AAT. These findings contribute to a better understanding of the complex and sometimes contradictory observations of NETs and AAT in antimicrobial effects.
Article
Microbiology
Alexander Eng, Hillary S. Hayden, Christopher E. Pope, Mitchell J. Brittnacher, Anh T. Vo, Eli J. Weiss, Kyle R. Hager, Daniel H. Leung, Sonya L. Heltshe, Daniel Raftery, Samuel Miller, Lucas R. Hoffman, Elhanan Borenstein
Summary: Comparing fecal microbial metagenomic content between healthy infants and those with CF revealed significant differences in functional capacities, intensifying during the first year of life. These differences, characterized by a delay in development of functional capacities in CF, were associated with metabolism, diet, antibiotic exposure, and specific microbial drivers. Furthermore, an integrated metagenomic and metabolomic analysis identified correlations between fecal GI metabolites and specific microbes in infants with CF, potentially impacting nutrition, growth, and GI health.
Article
Gastroenterology & Hepatology
Jessica Permain, Laura Appleton, Shaun S. C. Ho, Michael Coffey, Chee Y. Ooi, Jacqueline Keenan, Andrew S. Day
Summary: Fecal levels of CHI3L1 were higher in children with CF compared to healthy controls, without correlation to growth parameters or pancreatic insufficiency, indicating potential gut inflammation in CF patients that requires further investigation to confirm and determine long-term implications.
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2022)
Review
Pediatrics
Eduarda Sampaio Lazzarotto, Jannaina Ferreira de Melo Vasco, Fabiane Fuhr, Carlos Antonio Riedi, Nelson Augusto Rosario Filho
Summary: This study conducted a systematic review and qualitative synthesis of relevant literature, finding that fecal calprotectin can be used as an inflammatory marker for monitoring intestinal diseases, including cystic fibrosis, and as an aid in diagnosing and monitoring inflammatory bowel conditions in patients with cystic fibrosis.
JORNAL DE PEDIATRIA
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Andrew McCallister, Sang Hun Chung, Michael Antonacci, Margret Z. Powell, Agathe S. Ceppe, Scott H. Donaldson, Yueh Z. Lee, Rosa Tamara Branca, Jennifer L. Goralski
Summary: In CF subjects, ventilation abnormalities can be identified by both (19)F and (HPXe)-Xe-129 imaging, but these abnormalities are not entirely congruent. (19)F and (HPXe)-Xe-129 imaging provide complementary information that allows for differentiation of normally ventilated, slowly ventilated, and non-ventilated regions in the lungs.
MAGNETIC RESONANCE IN MEDICINE
(2021)
Article
Chemistry, Analytical
Zhongyue Guo, Yeran Bai, Meng Zhang, Lu Lan, Ji-Xin Cheng
Summary: Antimicrobial resistance poses a major threat to global health and economics, necessitating rapid AST methods. We introduce a rapid AST method based on wide-field mid-infrared photothermal imaging, capable of metabolic imaging for hundreds of bacteria at single-cell resolution within seconds. This method enables probing of perturbed microbial protein synthesis within 1 hour after antibiotic treatment in E. coli cells, providing insights into antibiotic susceptibility.
ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Shuo Liu, Xiaolong Dong, He Cao, Jiaqi Lv, Linwan Zhao, Yuanqin Xia, Yulei Wang, Zhiwei Lv
Summary: A six-ring node-less anti-resonant hollow-core fiber gas sensor is proposed for measuring the concentration of human exhaled nitric oxide in the mid-infrared band. The sensitivity and minimum detectable absorbance of the sensor are determined through theoretical simulations, and the detection ranges for different types of bronchitis and asthma are obtained by simulating the exhaled nitric oxide concentrations of different individuals.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Farid Imanzadeh, Fatemeh Kerami, Maryam Hassanzad, Amirhossein Hosseini, Mahmoud Hajipour, Ghamartaj Khanbabaee, Naghi Dara, Katayoun Khatami, Nazanin Farahbakhsh, Aliakbar Sayyari
Summary: This study found that changes in fecal calprotectin levels are associated with exacerbations of cystic fibrosis (CF) and antibiotic treatment can reduce calprotectin levels. Therefore, fecal calprotectin levels can be considered as a diagnostic tool and an index to monitor treatment response in CF exacerbations.
BMC GASTROENTEROLOGY
(2022)