A novel zero-dead-volume sample loading interface for microfluidic devices: flexible hydraulic reservoir (FHR)

Infusing minute amounts of valuable liquids such as samples to microfluidic chips by using common pumping schemes such as syringe pumps often result in an excessive dead-volume. We present a simple yet effective sample loading interface, which helps by pumping the sample to the chip by using the hydraulic pressure generated by the syringe pump. Results show that sample volumes as low as 25 mu l can be delivered at flow rates ranging between 10-30 mu l min(-1). Maximum dead volume ratio was observed to be 3% when infusing 200 mu l of sample at 10 mu l min(-1).