Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 151, Issue -, Pages 69-75Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2018.05.027
Keywords
Phenotypic identification; Label-free Raman spectroscopy; Single-cell analysis; Raman standardization
Categories
Funding
- Qindao Beibao Marine Science & Technology Co. Ltd, Qingdao West-coast economic new area, China.
- Special Research Fund from Ghent University [BOFSTA2015000501]
- Belgian Federal Science Policy (BelSpo) under the inter-university attraction programme mu-manager [IUAP P7/25]
- Agency for Innovation by Science and Technology (IWT Flanders) [SB-131370]
- Bijzonder Onderzoeksfonds (BOF) of UGent [24J201400010]
- BOF UGent
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Raman spectroscopy has gained relevance in single-cell microbiology for its ability to detect bacterial (sub) populations in a non-destructive and label-free way. However, the Raman spectrum of a bacterium can be heavily affected by abiotic factors, which may influence the interpretation of experimental results. Additionally, there is no publicly available standard for the annotation of metadata describing sample preparation and acquisition of Raman spectra. This article explores the importance of sample manipulations when measuring bacterial subpopulations using Raman spectroscopy. Based on the results of this study and previous findings in literature we propose a Raman metadata standard that incorporates the minimum information that is required to be reported in order to correctly interpret data from Raman spectroscopy experiments. Its aim is twofold: 1) mitigate technical noise due to sample preparation and manipulation and 2) improve reproducibility in Raman spectroscopy experiments studying microbial communities.
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