Article
Medicine, General & Internal
Tonci Sustic, Julie Van Coillie, Mads Delbo Larsen, Ninotska I. L. Derksen, Zoltan Szittner, Jan Nouta, Wenjun Wang, Timon Damelang, Ianthe Rebergen, Federica Linty, Remco Visser, Juk Yee Mok, Dionne M. Geerdes, Wim J. E. van Esch, Steven W. de Taeye, Marit J. van Gils, Leo van de Watering, C. Ellen van der Schoot, Manfred Wuhrer, Theo Rispens, Gestur Vidarsson
Summary: The study introduces a new method, FEASI, which can be widely applied in COVID-19 vaccine administration and antibody testing. FEASI can simultaneously measure IgG levels and fucosylation levels, and this method is accessible to all diagnostic and research laboratories.
Review
Oncology
Chengcheng Liao, Qian Wang, Jiaxing An, Jie Chen, Xiaolan Li, Qian Long, Linlin Xiao, Xiaoyan Guan, Jianguo Liu
Summary: The interaction of CD44, a non-kinase transmembrane glycoprotein, with ligands like hyaluronic acid plays a crucial role in tumor occurrence and development. Changes in CD44 glycosylation can regulate multiple signaling pathways, impacting the tumor microenvironment and cell fate. However, our current understanding of CD44 glycosylation and its effects on tumors is limited.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Xinqing Zhu, Abdullah Al-Danakh, Lin Zhang, Xiaoxin Sun, Yuli Jian, Haotian Wu, Dan Feng, Shujing Wang, Deyong Yang
Summary: Renal cell carcinoma is a common malignant tumor of the urinary system. Understanding the role of glycosylation in RCC may lead to the identification of novel targets and molecular markers for diagnosis and therapy.
Review
Cell Biology
Chiara De Vellis, Silvia Pietrobono, Barbara Stecca
Summary: Malignant melanoma, originating from the malignant transformation of melanocytes, is the most aggressive form of skin cancer. Aberrant glycosylation, including sialylation, fucosylation, and N- and I-glycan branching, is considered a hallmark of cancer and plays a role in promoting melanoma progression through activation of invasion and metastasis, immune evasion, and cell proliferation.
Article
Immunology
Marie Morel, Pierre Pochard, Wiam Echchih, Maryvonne Dueymes, Cristina Bagacean, Sandrine Jousse-Joulin, Valerie Devauchelle-Pensec, Divi Cornec, Christophe Jamin, Jacques-Olivier Pers, Anne Bordron
Summary: This study investigated the glycosylation of B cell subsets in systemic lupus erythematosus (SLE), primary Sjogren's syndrome (pSS), and healthy donors. The findings showed specific alterations in glycosylation profiles of B cells in both diseases, indicating that defective glycosylation may contribute to the development of pathogenic autoantibodies. The study also extended the glycan profile to serum proteins and immunoglobulins, further supporting the importance of glycosylation in autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Tomabu Adjobimey, Achim Hoerauf
Summary: This study investigates the link between total IgG-N-glycosylation patterns and the physiopathology of human lymphatic filariasis. The results reveal distinct N-linked IgG glycan profiles in different clinical groups, suggesting a correlation between IgG galactosylation and sialylation and chronic pathology, and agalactosylation and putative immunity. These findings emphasize the importance of N-glycosylation in lymphatic filariasis and its potential for developing new therapeutic formulations.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Mads Delbo Larsen, Erik L. de Graaf, Myrthe E. Sonneveld, H. Rosina Plomp, Jan Nouta, Willianne Hoepel, Hung-Jen Chen, Federica Linty, Remco Visser, Maximilian Brinkhaus, Tonci Sustic, Steven W. de Taeye, Arthur E. H. Bentlage, Suvi Toivonen, Carolien A. M. Koeleman, Susanna Sainio, Neeltje A. Kootstra, Philip J. M. Brouwer, Chiara Elisabeth Geyer, Ninotska I. L. Derksen, Gertjan Wolbink, Menno de Winther, Rogier W. Sanders, Marit J. van Gils, Sanne de Bruin, Alexander P. J. Vlaar, Theo Rispens, Jeroen den Dunnen, Hans L. Zaaijer, Manfred Wuhrer, C. Ellen van dDer Schoot, Gestur Vidarsson
Summary: This study reveals that afucosylated IgG antibodies are specifically formed against enveloped viruses, but not generally against other antigens. These antibodies enhance Fc gamma RIIIa responses, but also exacerbate cytokine storms and immune-mediated pathologies.
Article
Endocrinology & Metabolism
Priti Gupta, Tibor Saghy, Jauquline Nordqvist, Jonas Nilsson, Hans Carlsten, Karin Horkeby, Petra Henning, Cecilia Engdahl
Summary: This study aims to determine if estrogen levels can regulate IgG glycosylation in postmenopausal healthy situations.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Iwona Wojcik, David E. Schmidt, Lisa A. de Neef, Minke A. E. Rab, Bob Meek, Okke de Weerdt, Manfred Wuhrer, C. Ellen van der Schoot, Jaap J. Zwaginga, Masja de Haas, David Falck, Gestur Vidarsson
Summary: The spleen plays a crucial role in adaptive immune responses to viral infections and alloantigens by hosting a variety of immune cell populations. Splenectomy leads to an increase in IgG-Fc fucosylation, suggesting that the spleen may play a role in generating afucosylated IgG responses. Unexpectedly, ITP patients show a decrease in total IgG-Fc fucosylation compared to healthy controls, pointing to a potential role of the spleen in regulating afucosylated IgG responses.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Tyler Kim, Yixuan Xie, Qiongyu Li, Virginia M. Artegoitia, Carlito B. Lebrilla, Nancy L. Keim, Sean H. Adams, Sridevi Krishnan
Summary: The study reveals that glycosylation of proteins may be influenced by dietary patterns, with a healthier diet pattern associated with higher sialylation levels.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Review
Biochemistry & Molecular Biology
Kayla Bastian, Emma Scott, David J. Elliott, Jennifer Munkley
Summary: Aberrant glycosylation, particularly altered core fucosylation driven by FUT8, is a universal feature of cancer cells impacting tumor progression. Upregulated FUT8 plays critical roles in immune evasion and cancer development, outlining potential opportunities for future therapeutics targeting FUT8.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Zejian Zhang, Karli R. Reiding, Jianqiang Wu, Zepeng Li, Xiequn Xu
Summary: This study comprehensively evaluated the plasma N-glycomic changes in patients with TC or BTN for the first time, determining several N-glycan biomarkers with potential for differential diagnosis and risk assessment of lymph node metastasis. These findings enhance the understanding of TC.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Immunology
Iwona Wojcik, Manfred Wuhrer, Peter Heeringa, Coen A. Stegeman, Abraham Rutgers, David Falck
Summary: This study found that the glycosylation changes of IgG are associated with disease flares in patients with ANCA-associated vasculitis. In addition, among the glycosylation changes, alterations in Fc bisection and fucosylation levels of IgG are correlated with long-term treatment outcome and impending relapse.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pirjo Nummela, Annamari Heiskanen, Soili Kytola, Caj Haglund, Anna Lepisto, Tero Satomaa, Ari Ristimaki
Summary: Pseudomyxoma peritonei (PMP) is a highly mucinous adenocarcinoma originating most commonly from the appendix, and alterations in the sialic acid linkage pattern in PMP tumors may play a role in the dissemination and progression of the disease.
Article
Biotechnology & Applied Microbiology
Simon Joubert, Julie Guimond, Sylvie Perret, Felix Malenfant, S. Mehdy Elahi, Anne Marcil, Marie Parat, Michel Gilbert, Anne E. G. Lenferink, Jason Baardsnes, Yves Durocher
Summary: A method to enhance the effector function of antibodies and increase their antitumor activity by inhibiting core fucosylation is discussed. The expression of a membrane-associated anti-FUT8 intrabody in the endoplasmic reticulum and Golgi apparatus reduces FUT8 enzyme activity and core fucosylation of antibody N-glycans, resulting in antibodies with enhanced ADCC effector function.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Jelena Simunovic, Jernej Gaspersic, Urh Cernigoj, Jana Vidic, Ales Strancar, Mislav Novokmet, Genadij Razdorov, Marija Pezer, Gordan Lauc, Irena Trbojevic-Akmacic
Summary: This study developed a high-throughput method for the enrichment of haptoglobin from human plasma, using monolithic chromatographic support and fluorescent detection technology. The method allows for fast, efficient, and robust analysis of haptoglobin glycosylation.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Engineering, Multidisciplinary
Marina Kljakovic-Gaspic Batinjan, Tea Petrovic, Frano Vuckovic, Irzal Hadzibegovic, Barbara Radovani, Ivana Jurin, Lovorka Derek, Eva Huljev, Alemka Markotic, Ivica Luksic, Irena Trbojevic-Akmacic, Gordan Lauc, Ivan Gudelj, Rok Civljak
Summary: This study analyzed and compared the IgG glycosylation characteristics of patients with influenza, COVID-19, and healthy controls. The results showed significant decreases in IgG galactosylation, sialylation, and bisecting GlcNAc in deceased COVID-19 patients, while IgG fucosylation increased. Influenza patients and COVID-19 survivors had stable IgG glycosylation. The similarities in IgG glycosylation changes between COVID-19 survivors and influenza patients may indicate different immune responses to enveloped viruses.
Article
Biochemical Research Methods
Barbara Radovani, Gordan Lauc, Ivan Gudelj
Summary: Immunoglobulin G (IgG) is an important antibody in the blood that plays a critical role in the immune defense against infectious agents. The glycosylation of IgG has been found to affect its functions and is associated with disease development and progression. Saliva is an easily collectible sample and could be a useful tool for studying the glycosylation of salivary IgG and its potential as a diagnostic biomarker. This study describes a method for analyzing the glycosylation of IgG from saliva samples using ultra-high-performance liquid chromatography, and compares it with the glycosylation profiles of IgG from plasma. The stability of salivary IgG glycosylation under different storage conditions and the effects of using a saliva preservation medium were also assessed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Correction
Biochemical Research Methods
Moumen M. Alhasan, Sinisa Habazin, Fariz G. Kahhaleh, Gordan Lauc, Melanie L. Conrad, Mislav Novokmet
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Moumen M. M. Alhasan, Sinisa Habazin, Fariz G. G. Kahhaleh, Gordan Lauc, Melanie L. L. Conrad, Mislav Novokmet
Summary: Researchers developed a method to analyze IgG-Fc glycosylation in lactating mice and found that antibiotic use during pregnancy affects the glycosylation patterns of IgG in offspring serum. This work contributes to a better understanding of mother-infant immunological interactions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Alessia Visconti, Niccolo Rossi, Helena Deris, Karla A. Lee, Maja Hanic, Irena Trbojevic-Akmacic, Andrew M. Thomas, Laura A. Bolte, Johannes R. Bjork, Jahlisa S. Hooiveld-Noeken, Ruth Board, Mark Harland, Julia Newton-Bishop, Mark Harries, Joseph J. Sacco, Paul Lorigan, Heather M. Shaw, Elisabeth G. E. de Vries, Rudolf S. N. Fehrmann, Rinse K. Weersma, Tim D. Spector, Paul Nathan, Geke A. P. Hospers, Peter Sasieni, Veronique Bataille, Gordan Lauc, Mario Falchi
Summary: This study identifies novel glyco-markers of response and survival in melanoma patients receiving ICIs, demonstrating the potential for stratification of patients and the design of adjunct therapies.
Article
Engineering, Multidisciplinary
Julija Juric, Hongli Peng, Manshu Song, Frano Vuckovic, Jelena Simunovic, Irena Trbojevi-Akmacic, Youxin Wang, Jiaonan Liu, Qing Gao, Hao Wang, Qiaoyun Chu, Marija Pezer, Wei Wang, Gordan Lauc
Summary: The N-glycosylation of IgG exhibits cyclic periodic changes during the menstrual cycle, which are associated with the menstrual cycle phase and sex hormone concentration. While the average effect of the menstrual cycle on IgG N-glycosylation traits is low, there are significant intrapersonal variations. These findings are important for large sample-size studies on IgG N-glycosylation in women.
Article
Biochemistry & Molecular Biology
Maja Hanic, Frano Vuckovic, Helena Deris, Claire Bewshea, Simeng R. Lin, James Goodhand, Tariq Ahmad, Irena A. Trbojevic-Akmacic, Nicholas Kennedy, Gordan Lauc
Summary: Crohn's disease (CD) is a chronic inflammation of the digestive tract. Anti-TNF treatment can improve the condition, but its effectiveness varies among patients. This study used N-glycan analysis to investigate the effect of anti-TNF drugs on IgG composition and its potential in predicting therapy response. The results showed changes in IgG glycosylation patterns during treatment, suggesting reduced inflammation in CD patients. However, IgG glycome composition at baseline cannot solely predict future non-responders to anti-TNF therapy.
Article
Biochemistry & Molecular Biology
Nina Simunic-Briski, Robert Zekic, Vedran Dukaric, Mateja Ocic, Azra Frkatovic-Hodzic, Helena Deris, Gordan Lauc, Damir Knjaz
Summary: Regular exercise has a positive impact on the immune system and inflammatory status, as demonstrated by changes in IgG N-glycosylation. This study found that exercise intervention led to alterations in the composition of IgG N-glycome, including increases in certain glycan structures associated with inflammation and a protective cardiovascular role. These findings emphasize the importance of regular exercise for overall health and highlight its potential to modulate inflammatory responses.
Article
Biology
Irena Trbojevic-Akmacic, Frano Vuckovic, Tea Pribic, Marija Vilaj, Urh Cernigoj, Jana Vidic, Jelena Simunovic, Agnieszka Kepka, Ivana Kolcic, Lucija Klaric, Mislav Novokmet, Maja Pucic-Bakovic, Erdmann Rapp, Ales Strancar, Ozren Polasek, James F. F. Wilson, Gordan Lauc
Summary: A highly specific high-throughput approach for transferrin purification and characterization of N-glycosylation was developed. The N-glycosylation of transferrin is associated with age, sex, and various biochemical and physiological traits. The N-glycosylation patterns of transferrin differ from that of immunoglobulin G (IgG), indicating tissue-specific N-glycosylation and the specific role of N-glycans in distinct physiological functions.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sofia Shkunnikova, Anika Mijakovac, Lucija Sironic, Maja Hanic, Gordan Lauc, Marina Martinic Kavur
Summary: Immunoglobulin (IgG) glycosylation is a crucial process for the structure and function of IgG. Changes in IgG glycome have been associated with aging, pollution, diseases, and inflammation. IgG N-glycosylation fine-tunes the immune response and can be used as a biomarker and evaluation tool in various health interventions.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Medicine, General & Internal
Panayiotis Louca, Tamara Stambuk, Azra Frkatovic-Hodzic, Ana Nogal, Massimo Mangino, Sarah E. Berry, Helena Deris, George Hadjigeorgiou, Jonathan Wolf, Martina Vinicki, Paul W. Franks, Ana M. Valdes, Tim D. Spector, Gordan Lauc, Cristina Menni
Summary: This study explores the relationship between plasma protein N-glycosylation and postprandial metabolism, and finds that certain glycans are significantly associated with postprandial triglycerides, glucose, and insulin levels. Furthermore, some glycans mediate the relationship between prediabetes and postprandial triglycerides.
Article
Endocrinology & Metabolism
Elham Memarian, Ralph Heijmans, Roderick C. Slieker, Adriana Sierra, Olga Gornik, Joline W. J. Beulens, Maja Hanic, Petra Elders, Julio Pascual, Eric Sijbrands, Gordan Lauc, Viktoria Dotz, Clara Barrios, Leen M. 't Hart, Manfred Wuhrer, Mandy van Hoek
Summary: Inflammation plays an important role in the development of type 2 diabetes complications, and the N-glycosylation of IgG affects its role in inflammation. This study found that IgG N-glycosylation, particularly galactosylation and to a lesser extent sialylation, is associated with a higher prevalence and future development of macro- and microvascular complications of diabetes.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Fran Luksic, Anika Mijakovac, Goran Josipovic, Vedrana Vicic Bockor, Jasminka Kristic, Ana Cindric, Martina Vinicki, Filip Rokic, Oliver Vugrek, Gordan Lauc, Vlatka Zoldos
Summary: The glycosylation of IgG plays a regulatory role in its effector function in the immune response, making it a potent therapeutic option for research and clinical purposes. However, there is a lack of data regarding the stability of IgG glycome during long-term cell passaging. Our study using FreeStyle 293-F cells, which secrete IgG with a human-like glycosylation pattern, revealed that while most components of the IgG glycome remained stable during long-term cell culturing, galactosylation showed high variability.