Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jie Bao, Juan Chen, XiZhao Zhang, Li Xu, YongSheng Fan, XiaoBing Dou
Summary: This study aimed to explore the serum signatures of RAU patients through proteomic and transcriptomic analysis, revealing that complement and coagulation cascade pathway, oxidative stress, and inflammation responses may play vital roles in the pathogenesis of RAU. The study identified key miRNA-protein pairs associated with oxidative stress and inflammation responses, and highlighted the importance of the complement and coagulation cascade pathway in RAU.
Article
Chemistry, Medicinal
Oliver Fiehn, Parker Ladd Bremer, Arpana Vaniya, Tobias Kind, Shunyang Wang
Summary: CFM-ID is a machine learning tool for predicting MS/MS spectra of metabolites. Matching experimental collision energy with CFM-ID's predicted energy produced optimal results, especially for benzenoids on HCD-Orbitrap instruments. CFM-ID 4.0 could be useful as a supplementary tool in the broader context of identification workflows.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Multidisciplinary Sciences
Matthew Antony Manoj, Animesh Jain, Saanchia Andria Madtha, Tina Mary Cherian
Summary: This study investigated the prevalence and risk factors of recurrent aphthous stomatitis (RAS) among college students in Mangalore, Karnataka, India. The results showed significant associations between RAS and family history, diabetes, smoking, oral trauma, wearing braces/dentures, toothpastes with sodium lauryl sulphate, lack of sleep, and stress.
Article
Geriatrics & Gerontology
Fu-Chao Liu, Mei-Ling Cheng, Chi-Jen Lo, Wen-Chuin Hsu, Gigin Lin, Huan-Tang Lin
Summary: In this study, CSF metabolomics using LC-MS was employed to investigate the metabolic changes during aging. Several metabolites were found to be significantly correlated with age, and sex differences were also observed. These findings may provide valuable insights into healthy brain aging and warrant further exploration.
Article
Multidisciplinary Sciences
Gabriela Moraes Oliveira, Thiago Jose Dionisio, Nelson Leonel Del Hierro Polanco, Viviane Silva Siqueira-Sandrin, Flavio Augusto Cardoso Faria, Carlos Ferreira Santos, Adriana Maria Calvo
Summary: After analyzing samples from volunteers who took naproxen alone or in combination with esomeprazole, it was found that the combination took longer to reach the maximum concentration, had a longer elimination time, and lower maximum concentrations compared to naproxen alone. The AUC values and clearance rates also differed between the two situations.
Article
Chemistry, Analytical
Chiara Maria Vitale, Arjen Lommen, Carolin Huber, Kevin Wagner, Borja Garlito Molina, Rosalie Nijssen, Elliott James Price, Marco Blokland, Frederike van Tricht, Hans G. J. Mol, Martin Krauss, Laurent Debrauwer, Olga Pardo, Nuria Leon, Jana Klanova, Jean-Philippe Antignac
Summary: A set of QA/QC criteria for nontargeted measurement of pesticide exposure markers in human urine has been proposed and applied in the HBM4EU project. The criteria include monitoring signal intensities, mass accuracy, and retention times of selected markers. The results show high repeatability and accuracy of the data. This is the first time that harmonized QA/QC criteria have been used to assess the quality of nontargeted analysis of human samples across multiple laboratories.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Qin-Feng Zhang, Hua-Ming Xiao, Jin-Tao Zhan, Bi-Feng Yuan, Yu-Qi Feng
Summary: Intestinal microbes play a crucial role in converting ingested diet into physiologically active small molecules. This study presents a reversed-phase chromatography method that allows simultaneous determination of 20 indole metabolites of tryptophan, providing a useful tool for studying related diseases.
CHINESE CHEMICAL LETTERS
(2022)
Article
Immunology
Xiang Wang, Kexu Xiong, Fan Huang, Jinqun Huang, Qin Liu, Ning Duan, Huanhuan Ruan, Hongliu Jiang, Yanan Zhu, Lin Lin, Yuefeng Song, Maomao Zhao, Lichun Zheng, Pei Ye, Yajie Qian, Qingang Hu, Fuhua Yan, Wenmei Wang
Summary: Through analysis of RAU patients, gut dysbacteriosis, microbial dysfunction, and immune imbalance were found, and the regulatory effects of thalidomide on gut microbiota and immunity were determined. These findings suggest that targeting gut dysbacteriosis and modifying immune imbalance may affect the treatment of RAU.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Teresa S. McMillen, Andrew Leslie, Kenneth Chisholm, Susanne Penny, Jeffrey Gallant, Alejandro Cohen, Arik Drucker, James P. Fawcett, Devanand M. Pinto
Summary: This study presents a comprehensive and quantitatively robust method for metabolomic analysis, using HILIC and RP stationary phases combined with positive and negative electrospray ionization. The method allows for complete analysis of a single sample in as little as 40 minutes and has been validated with clinical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Gwang Bin Lee, Ayse Caner, Myeong Hee Moon
Summary: This study evaluated the methods for extracting and analyzing salivary lipids using nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry (nUHPL-CESI-MS/MS), and determined the minimum volume of saliva sufficient for lipidomic analysis to be 0.5 mL.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Alex Gomez-Gomez, MohammadA Sabbaghi, Noemi Haro, Joan Albanell, Silvia Menendez, Monica Gonzalez, Gabriel Gil-Gomez, Ana Rovira, Oscar J. Pozo
Summary: Formalin-fixed paraffin-embedded (FFPE) tissues are crucial in cancer research, but research on the detection of small molecules in FFPE samples is limited. This study aimed to explore the potential of targeted metabolomics in FFPE specimens and developed a LC-MS/MS method for the quantification of acidic metabolites. The method showed accurate and precise quantification of 40 metabolites in FFPE samples, with implications for cancer research.
Article
Chemistry, Analytical
Kristian Serafimov, Yuesra Aydin, Michael Laemmerhofer
Summary: In this study, a fast-targeted liquid chromatography-tandem mass spectrometry method was proposed for accurate analysis of metabolites from the glutathione pathway. The addition of N-Ethylmaleimide reagent to the extraction solvent quickly stabilized the thiol-redox state. This method demonstrated high throughput analysis, sensitivity, and accuracy.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Chemistry, Analytical
Joao Raul Belinato, Vinicius Ricardo Acquaro, Luciana Teresa Dias Cappelini, Fabio Augusto
Summary: The interest in bioactive compounds from microorganisms has increased since the first discovery, with microorganisms being a key source of biomolecules with diverse applications. While conventional sample preparation techniques are still widely used, microextraction techniques show promise in overcoming limitations and promoting a deeper understanding of complex systems and the discovery of new molecules.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Tahereh Molania, Jafar Akbari, Amirhossein Babaei, Anahita Lotfizadeh, Mahmood Moosazadeh, Abbas Mesgarani, Anahita Baasl, Maede Salehi
Summary: Through a clinical trial, it was found that atorvastatin mucoadhesive tablets can effectively reduce the pain of patients with minor recurrent aphthous stomatitis and decrease the size and healing time of the lesions. Therefore, the use of atorvastatin mucoadhesive tablets should be considered in the treatment of minor recurrent aphthous stomatitis.
Editorial Material
Cell Biology
D. Wang, X. Yang, X. Chai, J. Fang, X. Zhang
Article
Medicine, Research & Experimental
Jian Liu, Xuemei Qiu, Daoming Wang, Yantao Li, Yang Zong, Yichen Liu, Yue Zhang, Ping Yang, Yong Zuo, Huanming Yang, Jian Wang, Yutao Du, Jin Zi
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Daoming Wang, Yan Li, Huanzi Zhong, Qiuxia Ding, Yuxiang Lin, Shanmei Tang, Yang Zong, Qi Wang, Xiuqing Zhang, Huanming Yang, Rong Wang, Xiao Liu
Article
Biochemistry & Molecular Biology
Lianmin Chen, Daoming Wang, Sanzhima Garmaeva, Alexander Kurilshikov, Arnau Vich Vila, Ranko Gacesa, Trishla Sinha, Eran Segal, Rinse K. Weersma, Cisca Wijmenga, Alexandra Zhernakova, Jingyuan Fu
Summary: By tracking the gut microbiome, human phenotypes, and metabolites, researchers have developed a microbial fingerprinting method for classifying samples with high accuracy. The study suggests that the gut microbiome may influence cardiometabolic health through its metabolites, highlighting the importance of microbial stability and variation in relation to host physiology.
Article
Genetics & Heredity
Sergio Andreu-Sanchez, Lianmin Chen, Daoming Wang, Hannah E. Augustijn, Alexandra Zhernakova, Jingyuan Fu
Summary: Metagenomic sequencing is crucial for studying microbial communities, but identifying genetic variation in metagenomes remains challenging due to factors such as low sequencing depth and large genomic variation. While some bioinformatics tools have been developed for this purpose, their performance has not been systematically evaluated. Probabilistic tools like HaplotypeCaller and Mutect2 from the GATK toolset show the best performance in identifying genetic variants in metagenomic samples.
FRONTIERS IN GENETICS
(2021)
Article
Microbiology
Daoming Wang, Marwah Doestzada, Lianmin Chen, Sergio Andreu-Sanchez, Inge C. L. van den Munckhof, Hannah E. Augustijn, Martijn Koehorst, Angel J. Ruiz-Moreno, Vincent W. Bloks, Niels P. Riksen, Joost H. W. Rutten, Leo A. B. Joosten, Mihai G. Netea, Cisca Wijmenga, Alexandra Zhernakova, Folkert Kuipers, Jingyuan Fu
Summary: The study found correlations between genetic variations in gut microbiota and host bile acid metabolism, highlighting the potential of lifestyle interventions in regulating bile acid metabolism through targeting gut microbiota.
CELL HOST & MICROBE
(2021)
Article
Cell Biology
Anastasia Gulyaeva, Sanzhima Garmaeva, Renate A. A. A. Ruigrok, Daoming Wang, Niels P. Riksen, Mihai G. Netea, Cisca Wijmenga, Rinse K. Weersma, Jingyuan Fu, Arnau Vich Vila, Alexander Kurilshikov, Alexandra Zhernakova
Summary: In this study, gut metagenomic data from 1,950 individuals in the Netherlands were analyzed to explore the diversity and functional role of crAss-like phages in human populations. The researchers identified 1,556 crAss-like phage genomes and predicted their hosts to be bacteria of the phylum Bacteroidetes. The study also discovered striking divergent regions in the transcriptional regions of closely related crAss-like phages and explored the temporal stability of these phages over a 4-year period, finding associations between their abundance and several human phenotypes.
Article
Biochemistry & Molecular Biology
Daoming Wang, Tongda Zhang, Yueqi Lu, Changzheng Wang, Yumei Wu, Jiandong Li, Ye Tao, Le Deng, Xiaoyin Zhang, Jinmin Ma
Summary: This study investigated alterations in the gastric microbiome associated with Helicobacter pylori infection in the Chinese population. The results revealed distinct changes in the composition and diversity of the gastric microbiota in H. pylori-infected individuals, as well as the enrichment of specific microbial species and pathways.
Article
Biochemistry & Molecular Biology
Lianmin Chen, Daria Zhernakova, Alexander Kurilshikov, Sergio Andreu-Sanchez, Daoming Wang, Hannah E. Augustijn, Arnau Vich Vila, Rinse K. Weersma, Marnix H. Medema, Mihai G. Netea, Folkert Kuipers, Cisca Wijmenga, Alexandra Zhernakova, Jingyuan Fu
Summary: The study analyzed a large number of individuals to reveal the influence of an individual's genetics, diet, and gut microbiome on their plasma metabolome, and used Mendelian randomization and mediation analyses to unveil causal relationships between them.
Article
Medicine, General & Internal
Marwah Doestzada, Daria V. Zhernakova, Inge C. L. van den Munckhof, Daoming Wang, Alexander Kurilshikov, Lianmin Chen, Vincent W. Bloks, Martijn van Faassen, Joost H. W. Rutten, Leo A. B. Joosten, Mihai G. Netea, Cisca Wijmenga, Niels P. Riksen, Alexandra Zhernakova, Folkert Kuipers, Jingyuan Fu
Summary: This study systematically investigated the associations between branched-chain amino acids (BCAAs) and cardiometabolic diseases (CMD), and found significant correlations but no causal relationships between BCAAs and CMD parameters. Specific associations were identified for isoleucine, and the association of valine was influenced by obesity status. These findings highlight the clinical importance of individual BCAAs in the diagnosis, prevention, and treatment of CMD.
Article
Gastroenterology & Hepatology
Daoming Wang, Van T. Pham, Robert E. Steinert, Alexandra Zhernakova, Jingyuan Fu
Summary: This study systematically investigated the relationship between gut microbial vitamin production and factors related to diabetes and cardiometabolic health. The results showed that blood glucose-related factors, lipids, circulating inflammation, and fecal short-chain fatty acids are associated with gut microbial vitamin production.
Article
Multidisciplinary Sciences
Dingxue Hu, Yueqi Lu, Daoming Wang, Chao Nie, Yan Li
Summary: A study in 480 Chinese individuals investigated the genetic basis of host susceptibility to H. pylori infection, identifying several genes and pathways associated with infection. Analysis of gut microbiota revealed differential abundance of 2 species, 3 genera, and several pathways between H. pylori infected cases and controls. This study is the first GWAS for H. pylori infection in the Chinese population, providing insights into the genetic and microbial factors influencing susceptibility.
SCIENTIFIC REPORTS
(2021)
Article
Clinical Neurology
Yuanyuan Li, Bin Zhang, Ya Zhou, Daoming Wang, Xianchen Liu, Lin Li, Tong Wang, Yuechu Zhang, Min Jiang, Huilan Tang, Lawrence Amsel, Fang Fan, Christina W. Hoven
NATURE AND SCIENCE OF SLEEP
(2020)