Article
Chemistry, Multidisciplinary
Jonathan Cramer, Adem Lakkaichi, Butrint Aliu, Roman P. Jakob, Sebastian Klein, Ivan Cattaneo, Xiaohua Jiang, Said Rabbani, Oliver Schwardt, Gert Zimmer, Matias Ciancaglini, Tiago Abreu Mota, Timm Maier, Beat Ernst
Summary: DC-SIGN is a pattern recognition receptor implicated in virus dissemination and innate immune response stimulation, making it a potential target for host-directed strategies to attenuate the progression of viral infections. The discovery of potent glycomimetic ligands with improved binding affinity provides promising prospects for the development of targeted therapeutics against a broad spectrum of viral infections.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Virology
Federica Zavaglio, Loretta Fiorina, Nicolas M. Suarez, Chiara Fornara, Marica De Cicco, Daniela Cirasola, Andrew J. Davison, Giuseppe Gerna, Daniele Lilleri
Summary: This study investigated genotype-specific IgG antibody responses to HCMV gB and gH, finding that peptide-based ELISA can detect primary genotype-specific IgG responses, but about half of the subjects did not have genotype-specific IgG antibodies to gB.
Article
Biochemistry & Molecular Biology
Chu-Yen Huang, Yu-Chun Cheng, Yih-Shiou Hwang, Eugene Yu-Chuan Kang, Ching-Hsi Hsiao
Summary: This study investigated the distribution of CMV gB genotypes in anterior segment infection in Taiwan and their correlation with clinical manifestations and outcomes. A total of 57 patients with CMV anterior segment infection were studied. Results showed that multiple-genotype infection was correlated with higher CMV loads but not with outcomes, and the gB3 genotype was associated with a higher proportion of patients who had received filtering surgery before antiviral treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
A. C. Gomes, I. A. Baraniak, A. Lankina, Z. Moulder, P. Holenya, C. Atkinson, G. Tang, T. Mahungu, F. Kern, P. D. Griffiths, M. B. Reeves
Summary: Vaccination against CMV infection is highly prioritized, and a recombinant form of gB protein with MF59 adjuvant has shown partial protection in a clinical trial. However, neutralizing responses against known antigenic domains of gB were limited. We discovered that vaccination induces an antibody response against a region of gB called AD-6, which is detected in a majority of vaccine recipients but a minority of naturally infected individuals. The AD-6 antibody binds to gB and infected cells, preventing cell-cell spread of CMV but not directly neutralizing the virus. This finding has the potential to explain part of the protection provided by gB vaccines against CMV following transplantation.
NATURE COMMUNICATIONS
(2023)
Article
Pediatrics
Niuniu Dong, Lingfeng Cao, Danni Zheng, Liyun Su, Lijuan Lu, Zuoquan Dong, Menghua Xu, Jin Xu
Summary: This study revealed the distribution of gB, gH, and gN genotypes in CMV-infected infants in Shanghai for the first time. It showed a significant association between gH1 genotype and symptomatic CMV infection, an increased risk of petechiae with gB3 genotype, and a strong correlation between gN4a genotype and chorioretinitis in cCMV infection. No significant correlation was found between urine viral loads and CMV genotypes or hearing impairment in cCMV infected infants.
FRONTIERS IN PEDIATRICS
(2023)
Article
Biology
Rohan P. Choraghe, Aaron K. Neumann
Summary: Dectin-1 and DC-SIGN collaborate to enhance the capture and retention of Candida albicans, with Dectin-1 playing a crucial role in promoting the recruitment of DC-SIGN to the contact site.
Article
Immunology
Helen Webster, Sarah Valencia, Amit Kumar, Cliburn Chan, Maria Dennis, Hunter Roark, Angela Woods, Shinu John, Andrea Carfi, Sallie R. Permar
Summary: The study shows that species-specific CMV variant-specific antibody responses contribute to the immunogenicity of HCMV vaccines, indicating the necessity of considering pre-existing immunity in non-human primate models and the impact of different species' immune responses post-vaccination.
Article
Microbiology
Jennifer J. Jenks, Sharmi Amin, Madeline M. Sponholtz, Amit Kumar, Daniel J. Wrapp, Sravani Venkatayogi, Joshua Tu, Krithika S. Karthigeyan, Sarah Valencia, Megan R. Connors, Melissa Harnois, Bhavna F. Hora, Eric Rochat, Jason F. McLellan, Kevin Wiehe, Sallie F. Permar
Summary: This study identified a rare heavy chain mutation that confers neutralizing function and plays a key role in the gB AD-2S1 epitope. Lineage-based vaccine design may be used to induce potent neutralizing antibodies against CMV gB AD-2S1.
Article
Biochemistry & Molecular Biology
Tatsuya Hasegawa, Tomonori Oka, Heehwa G. Son, Valeria S. Oliver-Garcia, Marjan Azin, Thomas M. Eisenhaure, David J. Lieb, Nir Hacohen, Shadmehr Demehri
Summary: Senescent fibroblast accumulation in aging human skin is controlled by a virus-immune axis. Senescent fibroblasts increase in old skin, but not in the elderly, which is associated with increased recruitment of CXCL9 and cytotoxic CD4+ T cells. Skin-resident CD4 CTLs eliminate senescent fibroblasts expressing HLA-II and HCMV-gB through an HLA-II-dependent mechanism.
Article
Virology
Nancy Palmerin, Farizeh Aalam, Romina Nabiee, Murali Muniraju, Javier Gordon Ogembo, Jennifer Totonchy
Summary: This study assesses the contributions of KSHV glycoprotein H and the cellular receptor DC-SIGN to KSHV infection in B lymphocytes. Results show that DC-SIGN plays a role in KSHV entry into tonsil-derived B cells, but multiple entry mechanisms exist.
Article
Multidisciplinary Sciences
Yeon-Sook Choi, Myung Ji Kim, Eun A. Choi, Sinae Kim, Eun Ji Lee, Min Ji Park, Mi-Ju Kim, Yeon Wook Kim, Hee-Sung Ahn, Jae Yun Jung, Gayoung Jang, Yongsub Kim, Hyori Kim, Kyunggon Kim, Jin Young Kim, Seung-Mo Hong, Song Cheol Kim, Suhwan Chang
Summary: Gal-3BP is identified as a highly secreted protein in PDAC and its overexpression is closely related to cell proliferation, migration, and invasion. It is found that Gal-3BP enhances galectin-3-mediated signaling, leading to increased PDAC metastasis. Blocking Gal-3BP with antibodies can effectively suppress PDAC metastasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Saima Siddiqui, Sarah Hackl, Hamid Ghoddusi, Megan R. McIntosh, Ariane C. Gomes, Joshua Ho, Matthew B. Reeves, Gary R. McLean
Summary: This study compared IgG and IgA responses to gB and the AD-2 epitope in naturally exposed individuals and those receiving a recombinant gB/MF59 adjuvant vaccine. Vaccination improved pre-existing gB-specific IgA and IgG levels in sero-positive individuals, while inducing de novo gB-specific IgA and IgG responses in sero-negative recipients. Pre-existing AD-2 IgG and IgA responses were boosted with vaccination, but new AD-2 responses were not detected.
Article
Chemistry, Medicinal
Jonathan Cramer, Butrint Aliu, Xiaohua Jiang, Timothy Sharpe, Lijuan Pang, Adrian Hadorn, Said Rabbani, Beat Ernst
Summary: Research has shown that mannose-functionalized poly-L-lysine glycoconjugates efficiently inhibit viral attachment and prevent virus binding to DC-SIGN receptors. After treatment, these polymers can lead to prolonged receptor internalization and inhibition of virus binding for up to 6 hours.
Article
Biochemistry & Molecular Biology
Xinyi Zhang, Peiyu Liang, Yahui Zhang, Yifan Wu, Yinghao Song, Xueyang Wang, Taoxiang Chen, Biwen Peng, Wanhong Liu, Jun Yin, Song Han, Xiaohua He
Summary: This study discovered the upregulation of Kv1.3 in microglia in a mouse model of epilepsy and demonstrated that blocking Kv1.3 can alleviate seizure severity, prolong seizure latency, and reduce neuronal loss. These findings reveal the critical role of microglial Kv1.3 in epilepsy and provide a potential therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yeon-Sook Choi, Myung Ji Kim, Eun A. Choi, Sinae Kima, Eun Ji Lee, Min Ji Park, Mi-Ju Kim, Yeon Wook Kim, Hee-Sung Ahn, Jae Yun Jung, Gayoung Jang, Yongsub Kim, Hyori Kim, Kyunggon Kim, Jin Young Kim, Seung-Mo Hong, Song Cheol Kim, Suhwan Chang
Summary: This study identified Gal-3BP as a highly secreted protein in pancreatic ductal adenocarcinoma (PDAC) and demonstrated its importance in PDAC cell proliferation, migration, and invasion. Mechanistically, Gal-3BP was found to enhance galectin-3-mediated signaling, resulting in increased PDAC metastasis. The development of antibodies targeting Gal-3BP profoundly suppressed PDAC metastasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Samuel Alizon, Christian Selinger, Mircea T. Sofonea, Stephanie Haim-Boukobza, Jean-Marc Giannoli, Laetitia Ninove, Sylvie Pillet, Vincent Thibault, Alexis de Rougemont, Camille Tumiotto, Morgane Solis, Robin Stephan, Celine Bressollette-Bodin, Maud Salmona, Anne-Sophie L'honneur, Sylvie Behillil, Caroline Lefeuvre, Julia Dina, Sebastien Hantz, Cedric Hartard, David Veyer, Heloise M. Delagreverie, Slim Fourati, Benoit Visseaux, Cecile Henquell, Bruno Lina, Vincent Foulongne, Sonia Burrel
Summary: This study explores the use of Cq values from SARS-CoV-2 screening tests to better understand the spread of an epidemic and the biology of the infection. The results show significant associations between Cq values and patient age, number of days after symptom onset, and the state of the epidemic. The study suggests that Cq values can improve short-term predictions for epidemic surveillance.
Article
Virology
Karsten Eichholz, Tuan Hiep Tran, Coraline Cheneau, Thi Thu Phuong Tran, Oceane Paris, Martine Pugniere, Eric J. Kremer
Summary: This study examines the interactions between danger-associated molecular patterns and human adenoviruses and their impact on vaccines. The interaction between human adenoviruses and HNP-1 can modify the response of antigen-presenting cells, influencing vaccine efficacy. HNP-1 binds to the capsids of human adenoviruses and directs them to Toll-like receptor 4 (TLR4), leading to internalization, inflammasome response, and IL-1β release.
JOURNAL OF VIROLOGY
(2022)
Article
Critical Care Medicine
Tanguy Chaumette, Raphael Cinotti, Alice Molle, Pierre Solomon, Louise Castain, Cynthia Fourgeux, Hamish E. G. McWilliam, Barbara Misme-Aucouturier, Alexis Broquet, Cedric Jacqueline, Mickael Vourc'h, Delphine Fradin, Celine Bossard, Laurent David, Emmanuel Montassier, Cecile Braudeau, Regis Josien, Jose A. Villadangos, Karim Asehnoune, Celine Bressollette-Bodin, Jeremie Poschmann, Antoine Roquilly
Summary: Brain injury induces immunosuppression, increasing the risk of viral reactivations and impairing neurological recovery. This study found that lung replication of herpesviridae is associated with poor recovery after brain injury, and a specific monocyte signature can predict the neurological outcome at 6 months.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Public, Environmental & Occupational Health
C. Ferriot, C. Durance, L. Trutt, C. Rozo, C. Louvigne, C. Bressollette-Bodin, G. Birgand
Summary: This study investigated a COVID-19 super-spreading event involving the beta and delta variants of SARS-CoV-2 in a choir in a mental health center. The results showed that eight out of thirteen participants tested positive, with seven cases of delta variant and one case of beta variant. Additionally, 21 subsequent cases with suspected delta variant and two cases with suspected beta variant were identified among the contacts. The study highlights the underestimation of infectious risks and low perceived vulnerability as contributing factors to the super-spreading event.
JOURNAL OF HOSPITAL INFECTION
(2022)
Article
Immunology
Malte Deseke, Francesca Rampoldi, Inga Sandrock, Eva Borst, Heike Boning, George Liam Ssebyatika, Carina Jurgens, Nina Pluckebaum, Maleen Beck, Ahmed Hassan, Likai Tan, Abdi Demera, Anika Janssen, Peter Steinberger, Christian Koenecke, Abel Viejo-Borbolla, Martin Messerle, Thomas Krey, Immo Prinz
Summary: Deseke et al. identified HLA-DR as the cognate TCR ligand of a CMV-induced V delta 1(+) gamma delta T cell clone using soluble gamma delta TCRs and CRISPR/Cas9-mediated screening. This study provides insights into the potential role of HLA-DR in adaptive immune responses of gamma delta T cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Christophe Masset, Paul Le Turnier, Celine Bressollette-Bodin, Karine Renaudin, Francois Raffi, Jacques Dantal
Summary: Viral infections can cause severe kidney injuries, and the diagnosis and treatment should be based on the severity of the infection, drug toxicity, and direct or indirect viral-associated nephropathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Dorian McIlroy, Cecile Peltier, My-Linh Nguyen, Louise Manceau, Lenha Mobuchon, Nicolas Le Baut, Ngoc-Khanh Nguyen, Minh-Chau Tran, The-Cuong Nguyen, Celine Bressollette-Bodin
Summary: Mutations in the BKPyV capsid accumulate in kidney transplant recipients, and are associated with neutralization escape. The mutations are mainly caused by cytosine deamination by APOBEC3A/B enzymes.
Article
Microbiology
Eva Maria Borst, Sarah Harmening, Saskia Sanders, Enrico Caragliano, Karen Wagner, Tihana Lenac Rovis, Stipan Jonjic, Jens Bernhard Bosse, Martin Messerle
Summary: In human cytomegalovirus (HCMV), the small capsid protein (SCP) plays an essential role in ensuring stable levels of major capsid protein (MCP) and their mutual dependence guarantees appropriate levels of the other respective protein. The study provides insights into the mechanism of capsid assembly and identifies SCP as a potential target for antiviral inhibitors.
Article
Immunology
Amelie Rousseliere, Nathalie Gerard, Laurence Delbos, Pierrick Guerif, Magali Giral, Celine Bressollette-Bodin, Beatrice Charreau
Summary: The human cytomegalovirus (HCMV) triggers immune responses, including CD8(+) alpha beta T cells that recognize HCMV UL40 leader peptide restricted by HLA-E molecules. This study investigated the frequency, phenotype, and functions of HLA-E-UL40 CD8T cells in different infection states. The research found that HLA-E-UL40 CD8T cells share similar features with HLA-A2(pp65) CD8T cells but also have some differences.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Eleonora Forte, Ming Li, Fatma Ayaloglu Butun, Qiaolin Hu, Eva Maria Borst, Matthew J. Schipma, Andrea Piunti, Ali Shilatifard, Scott S. Terhune, Michael Abecassis, Jeffery L. Meier, Mary Hummel
Summary: This study investigates the role of immediate early proteins in regulating the viral epigenome, showing that these proteins play a critical role in recruiting Pol II and H3K27Ac to a specific element, leading to increased gene expression.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Ngoc-Khanh Nguyen, Marie -Claire Devilder, Laetitia Gautreau-Rolland, Cynthia Fourgeux, Debajyoti Sinha, Jeremie Poschmann, Maryvonne Hourmant, Celine Bressollette-Bodin, Xavier Saulquin, Dorian McIlroy
Summary: The BK polyomavirus (BKPyV) is a pathogen that causes significant morbidity in immunosuppressed individuals. This study identified broadly neutralizing antibodies against BKPyV and found a highly diverse BKPyV-specific repertoire of B cells with extensive somatic hypermutation. The study also identified three broadly neutralizing clonotypes that predominantly used IgG, indicating preferential targeting of specific BKPyV capsid epitopes by IgG.
LIFE SCIENCE ALLIANCE
(2023)
Article
Chemistry, Multidisciplinary
Vanessa Porkolab, Martin Lepsik, Stefania Ordanini, Alexander St John, Aline Le Roy, Michel Thepaut, Emanuele Paci, Christine Ebel, Anna Bernardi, Franck Fieschi
Summary: DC-SIGN functions as the coreceptor for SARSCoV-2 virus, and a glycomimetic ligand called Polyman26 inhibits DC-SIGN-dependent trans-infection. By designing dendrimer constructs that can engage two binding sites of the tetrameric DC-SIGN simultaneously, the researchers were able to achieve nanomolar affinity. This study opens up possibilities for developing new virus attachment blockers for different C-type lectin receptors.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Irene Herrera-Gonzalez, Manuel Gonzalez-Cuesta, Michel Thepaut, Eugenie Laigre, David Goyard, Javier Rojo, Jose M. Garcia Fernandez, Franck Fieschi, Olivier Renaudet, Pedro M. Nieto, Carmen Ortiz Mellet
Summary: The carbohydrate chemical mimicry of sp2-iminosugars enables the synthesis of high-mannose-type oligosaccharide analogs whose solution and lectin bound conformations match those of the natural partners. The ability to finely modulate the binding affinity and selectivity towards specific lectins by incorporating the OMJ sp2-iminosugar caps offers potential applications in various fields.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Multidisciplinary Sciences
Massilia Abbas, Meriem Maalej, Ferran Nieto-Fabregat, Michel Thepaut, Jean-Philippe Kleman, Isabel Ayala, Antonio Molinaro, Jean-Pierre Simorre, Roberta Marchetti, Franck Fieschi, Cedric Laguri
Summary: Lipopolysaccharides, a characteristic of gram-negative bacteria, play a crucial role in bacterial integrity. The immune system recognizes lipopolysaccharides through C-type lectin receptors. A study on macrophage galactose lectin reveals a unique structure that allows high-affinity binding to lipopolysaccharides on the bacterial surface.
Article
Infectious Diseases
Samuel Alizon, Christian Selinger, Mircea T. Sofonea, Stephanie Haim-Boukobza, Jean-Marc Giannoli, Laetitia Ninove, Sylvie Pillet, Vincent Thibault, Alexis de Rougemont, Camille Tumiotto, Morgane Solis, Robin Stephan, Celine Bressollette-Bodin, Maud Salmona, Anne-Sophie L'honneur, Sylvie Behillil, Caroline Lefeuvre, Julia Dina, Sebastien Hantz, Cedric Hartard, David Veyer, Heloise M. Delagreverie, Slim Fourati, Benoit Visseaux, Cecile Henquell, Bruno Lina, Vincent Foulongne, Sonia Burrel
Summary: This study utilizes a large database of COVID-19 screening test Cq values and identifies significant trends related to patient characteristics, disease progression, and epidemic status. The findings suggest that Cq values can contribute to improved short-term predictions for epidemic surveillance.