Journal
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
Volume 63, Issue -, Pages 103-111Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2018.02.005
Keywords
Cell penetrating peptide; siRNA delivery; Covalent CPP-siRNA chemical conjugate; Cell uptake mechanism; Cytotoxicity
Funding
- National Key Research and Development Plan [2016YFE0119200]
- National Natural Science Foundation of China (NSFC) [81361140344]
- Tianjin Municipal Science and Technology Commission [15JCYBJC28700, 15JCQNJC13600, 17JCYBJC20700]
- China Postdoctoral Science Foundation [2015M581306]
Ask authors/readers for more resources
The covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical translation. Cellular uptake mechanism study indicated that the conjugate could be taken up by cells via multiple pathways, including direct penetration of the plasma membrane and clathrin- and caveolae-independent endocytosis. In vitro cytotoxicity study revealed that the conjugation promoted internalization in a low-toxic fashion. (C) 2018 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available