Journal
JOURNAL OF IMMUNOLOGY
Volume 201, Issue 2, Pages 604-614Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1701322
Keywords
-
Categories
Funding
- Francis Crick Institute
- Cancer Research UK [FC001076/FC001999]
- UK Medical Research Council [FC001076/FC001999]
- Wellcome Trust [FC001076/FC001999]
- Wellcome Trust Career Development Fellowship [091664/B/10/Z]
- Foundation for Experimental Biomedicine (Zurich, Switzerland)
- Deutsche Forschungsgemeinschaft [VO 1602/6-1]
- MRC [MC_UP_1202/12] Funding Source: UKRI
Ask authors/readers for more resources
IFN-stimulated gene (ISG) 15 is a ubiquitin-like protein induced after type I IFN stimulation. There is a dearth of in vivo models to study free unconjugated ISG15 function. We found that free ISG15 enhances the production of IFN-gamma and IL-1 beta during murine infection with Toxoplasma gondii. In our model, ISG15 is induced in a type I IFN-dependent fashion and released into the serum. Increased ISG15 levels are dependent on an actively invading and replicating parasite. Two cysteine residues in the hinge domain are necessary determinants for ISG15 to induce increased cytokine levels during infection. Increased ISG15 is concurrent with an influx of IL-1 beta-producing CD8 alpha(+) dendritic cells to the site of infection. In this article, we present Toxoplasma infection as a novel in vivo murine model to study the immunomodulatory properties of free ISG15 and uniquely link it to IL-1 beta production by CD8 alpha(+) dendritic cells driven by two cysteines in the hinge region of the protein.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available