Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 453, Issue -, Pages 11-19Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2017.07.014
Keywords
Thrombosis; Sickle cell disease; Mass cytometry; Immunology; Signaling; Phospho-protein
Categories
Funding
- NHLBI NIH HHS [K23 HL119351, R01 HL142671] Funding Source: Medline
- NIH HHS [S10 OD023547] Funding Source: Medline
Ask authors/readers for more resources
Sickle cell disease (SCD) is a genetic disease caused by mutations in the beta globin gene, and inflammation plays a key role in driving many aspects of disease pathology. Early immune activation is believed to be associated with hemodynamic stresses and thrombus formation as cells traffic through blood vessels. We applied an extracorporeal perfusion system to model these effects ex vivo, and combined this with a phospho-CyTOF workflow to comprehensively evaluate single-cell signatures of early activation across all major circulating immune subsets. These approaches showed immune activation following passage through the perfusion chamber, most notably in monocytes, which exhibited platelet aggregation and significantly elevated expression of multiple phospho-proteins. Overall, these studies outline a robust and broadly applicable workflow to leverage phospho-CyTOF to characterize immune activation in response to ex vivo or in vivo perturbations and may facilitate identification of novel therapeutic targets in SCD and other inflammatory diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available